Tag: nevin manimala

Environ Toxicol Chem. 2021 Dec 16. doi: 10.1002/etc.5273. Online ahead of print.

ABSTRACT

The post-ecdysial mineralization in crustaceans involves the deposition of carbonate salts, such as calcium carbonate, to the organic matrix. Due to the resemblance between Pb²⁺ and Ca²⁺ , this study was carried out to investigate whether Pb is incorporated into the new shell during post-ecdysial mineralization using the blue crab, Callinectes sapidus, as the model crustacean. It was hypothesized that injected Pb would be deposited in the shell via calcium transporters in the epidermis during the mineralization process. Post-ecdysial blue crabs were injected with two doses of 5 µg Pb/g wet weight each in lead acetate and then Pb, Ca and Mg content were analyzed in the exoskeleton, while only Pb bioaccumulation was quantified for the hepatopancreas, gills, muscles, and hemolymph. Results showed a statistically non-significant increase in exoskeletal Pb content in Pb-treated crabs compared to control, suggesting that exoskeletal Pb may not be a sensitive proxy for aquatic Pb pollution. There was a significant decrease in Ca content in Pb-treated crabs, suggesting that Pb hindered the deposition of Ca to crab exoskeleton, thereby obstructing calcification. A trend of decrease in exoskeletal Mg was also observed in Pb-treated crabs. There was a significant increase in Pb content found in the gills, hepatopancreas, muscle, and hemolymph in Pb-treated crabs. The rank of the Pb level amongst three soft tissues in a decreasing order is: hepatopancreas > gill > muscle. This study is the first to present evidence that Pb disrupts post-ecdysial exoskeletal calcification in a crustacean. This article is protected by copyright. All rights reserved.

PMID:34913519 | DOI:10.1002/etc.5273

Gerodontology. 2021 Dec 16. doi: 10.1111/ger.12612. Online ahead of print.

ABSTRACT

INTRODUCTION: Malnutrition and risk of malnutrition continues to be a common finding in elders, yet its association with oral function in hospitalised patients remains unclear.

MATERIAL AND METHODS: Patients aged 70 years or over who had been hospitalised for non-acute rehabilitation were recruited. Nutritional risk was screened using the Mini-Nutritional Assessment Short Form (MNA-SF) and Nutritional Risk Screening (NRS) scores. Malnutrition was assessed according to the Global Leadership Initiative on Malnutrition (GLIM) criteria. All participants underwent the oral hypofunction test battery, evaluating oral hygiene, oral dryness, occlusal force, tongue-lip motor function, tongue pressure, masticatory and swallowing function. Statistical analyses comprised Mann-Whitney or Kruskal-Wallis tests. Bivariate associations between categorical variables were tested using the Pearson chi-square test; for continuous variables, the Spearman correlation was calculated. A P-value < .05 was considered statistically significant.

RESULTS: Sixty patients aged a mean 82.5 ± 7.0 years participated. Some 88.3% were diagnosed with oral hypofunction, and this was more common in older patients (P = .020). Analysing the 7 oral hypofunction tests as an interval variable (NiOF) revealed additional correlations with number of teeth (ρ = 0.477) as well as the nutritional risk, evaluated by the MNA-SF (ρ = -0.284) and NRS (ρ = 0.317) scores. NiOF scores were higher among denture wearers (P = .003). GLIM did not confirm the correlation with NiOF. Biomarkers such as serum albumin and CRP were not associated with the NiOF score.

CONCLUSION: In this sample, the association between oral function and nutritional state is more obvious in nutritional risk scores than in the malnutrition diagnosis by GLIM.

PMID:34913521 | DOI:10.1111/ger.12612

Artif Organs. 2021 Dec 16. doi: 10.1111/aor.14136. Online ahead of print.

ABSTRACT

Around 5% of coronavirus disease 2019 (COVID-19) patients develop critical disease, with severe pneumonia and acute respiratory distress syndrome (ARDS). In these cases, extracorporeal membrane oxygenation (ECMO) may be considered when conventional therapy fails. This study aimed to describe the clinical characteristics and in-hospital outcomes of COVID-19 patients with ARDS refractory to lung-protective ventilation and prone positioning on ECMO support, as well as to review the available literature on ECMO use and COVID-19 patients’ outcome. Patients from this case series were selected from the Brazilian COVID-19 Registry. From the 7646 patients included in the registry, only eight received ECMO support (0.1%), in four hospitals. The median age of the entire sample was 59 (interquartile range 54.2-64.4) years old and 87.5% were male. Hypertension (50.0%), diabetes mellitus (50.0%) and obesity (37.5%) were the most frequent comorbidities. The indications for ECMO were PaO₂ /FiO₂ ratio <80 mm Hg for more than 6 h or PaO₂ /FiO₂ ratio <60 mm Hg for more than 3 h. The mortality rate was 87.5%. In conclusion, in this case series of COVID-19 patients with ARDS refractory to conventional therapy who received ECMO support, a very high mortality was observed. Our findings are not different from previous studies including a small number of patients; however, there is a huge difference from Extracorporeal Life Support Organization results, which encourages us to keep looking for improvement.

PMID:34913492 | DOI:10.1111/aor.14136

J Ultrasound Med. 2021 Dec 16. doi: 10.1002/jum.15917. Online ahead of print.

ABSTRACT

OBJECTIVES: Increased ovarian stromal area (SA), stromal-to-ovarian area ratio (S/A), and echogenicity (SEcho) on ultrasonography have been proposed as diagnostic markers for polycystic ovary syndrome. Although several methods to evaluate the stroma exist, their reproducibility has not been defined which limits clinical utility. This study aimed to determine the interrater reliability and agreement of methods to evaluate SA, S/A, and SEcho.

METHODS: Five raters tested 3 methods to obtain SA and S/A, and one to obtain SEcho on 30 ovarian cineloops under two imaging conditions, simulating real-time (free-choice) or offline (fixed-frame) imaging. For SA, Method 1 subtracted follicular area from the ovarian area, Method 2 involved outlining the periphery of the stroma, and Method 3 represented a hybrid approach in which central follicles were subtracted from the outlined stroma. SEcho was scored on a subjective 3-tiered scale. Intraclass correlation coefficients (ICCs) and the coefficient of variation were determined for SA and S/A, and Fleiss’ kappa agreement statistics (κ) were determined for SEcho.

RESULTS: Interrater reliability of SA was superior using Method 1 (ICC = 0.558 and ICC = 0.705) versus Method 2 (ICC = 0.522 and ICC = 0.230) or Method 3 (ICC = 0.429 and ICC = 0.305) under free-choice and fixed-frame imaging conditions, respectively. Interrater reliability of S/A was also moderate to poor across methods. SEcho was also not reliably assessed across raters (κ = <0.500).

CONCLUSIONS: Ultrasonographic assessments of the ovarian stroma were associated with moderate to poor reproducibility. Indirect estimates of the ovarian stroma (Method 1) could be optimized to yield a reproducible approach, clarifying the clinical relevance of the stroma.

PMID:34913507 | DOI:10.1002/jum.15917

Fam Process. 2021 Dec 16. doi: 10.1111/famp.12736. Online ahead of print.

ABSTRACT

This article reports results of a study that assessed the efficacy of multiple family therapy (MFT) for helping children of depressed parent(s), using a quasi-randomized controlled trial design. In total, 76 children participated in the study, with 51 children were assigned to the experimental group (EG) and 25 to the comparison group (CG). The EG children and their parents completed the three-month MFT program, while the CG children and their parents attended two psychoeducational talks scheduled at the same time as the MFT. A group × Time repeated measure ANCOVA did not discern the intervention types having any effect on children’s lives in the post-treatment phase or at the three-month follow-up. However, the MFT brought some promising positive changes in the EG children’s perceived social support, both overall and that from the father and other family members at three-month follow-up; compared to the CG children, the EG children also attached more importance to the support from their mothers in the post-treatment phase and that from other family members at the three-month follow-up. The results implied the potential efficacy of the MFT in facilitating an increase in the overall social support of children of depressed parents and their positive interactions with both the healthy and the depressed parent and other family members. Owing to multiple statistical limitations, caution is required while interpreting the results. A larger sample and a more sophisticated research design were suggested for future studies examining the efficacy and therapeutic mechanism of the MFT.

PMID:34913487 | DOI:10.1111/famp.12736

Dan Med J. 2022 Dec 15;69(1):A05210397.

ABSTRACT

INTRODUCTION: In penile cancer, both disease and treatment may be mutilating. We examined life satisfaction in three separate groups; at penile cancer diagnosis and one and two years later.

METHODS: From 1 January 2013 to 31 December2015, Danish penile cancer patients completed the Life-Satisfaction Questionnaire-11 (LISAT-11) at diagnosis and after one year and after two years. Responders scored 11 domains of life on a six-point scale. We analysed the scores and compared scores with those of a Swedish control cohort from the literature with patients in the same age group.

RESULTS: A total of 157 individual penile cancer patients completed the LISAT-11 checklist at one point in the trajectory and were considered for this study. We observed trends towards less life satisfaction in the years after diagnosis and with increasingly mutilating surgical treatment. The differences were not statistically significant. A difference was observed between the proportion of responders scoring “satisfied” on activities of daily living between penile cancer patients and a healthy control cohort, with a higher score being recorded for the healthy controls. No other LISAT-11 domains were scored significantly different between penile cancer patients and controls.

CONCLUSIONS: Danish responders with penile cancer are less satisfied than a Swedish control cohort within the self-care domain.

FUNDING: none.

TRIAL REGISTRATION: not relevant.

PMID:34913430

Dis Aquat Organ. 2021 Dec 16;147:111-126. doi: 10.3354/dao03639.

ABSTRACT

High maternal investment and extended inter-calving intervals in Florida manatees Trichechus manatus latirostris make calf survivorship critical to overall population growth. However, detailed patterns of causes of mortality in calves have not been reported and state agency statistics report portions of perinatal mortality based on body length rather than actual cause of death (COD). The objectives of this study were to categorize COD based on necropsy data and geographical location in Florida for 1209 manatee calf carcasses (<236 cm total length) examined between January 2009 and December 2017 and to describe factors contributing to calf mortality. Results indicated COD was attributed to natural causes (47%, n = 573), cold stress syndrome (38%, n = 457), watercraft injury (13%, n = 155), or other human-related causes (2%, n = 24). Natural causes were the leading COD for small calves <151 cm, with death due to stillbirth or dystocia most frequent (48%, n = 273/573). Enteric trematodiasis contributed to a large proportion of deaths from natural causes in large calves within the southwest region of Florida, with an increasing annual trend. Brevetoxicosis contributed substantially to natural causes within the southwest region exclusively and was commonly comorbid with enteric trematodiasis. Cold stress syndrome was the leading cause of death for large calves (151-235 cm), with the Atlantic region having the highest proportion of cases. Watercraft injury was a sustained threat to large calves, especially within the southwest region. This report provides details on specific health threats and patterns of mortality among manatee calves.

PMID:34913440 | DOI:10.3354/dao03639

Infect Control Hosp Epidemiol. 2021 Dec 16:1-4. doi: 10.1017/ice.2021.482. Online ahead of print.

ABSTRACT

The CLEAR Trial recently found that decolonization reduced infections and hospitalizations in MRSA carriers in the year following hospital discharge. In this secondary analysis, we explored whether decolonization had a similar benefit in the subgroup of trial participants who harbored USA300, using two different definitions for the USA300 strain-type.

PMID:34913422 | DOI:10.1017/ice.2021.482

Dan Med J. 2022 Dec 7;69(1):A10210765.

ABSTRACT

INTRODUCTION: As the protection from the COVID-19 vaccines diminishes over time, health authorities are currently considering how to maintain immunity by means of vaccine booster doses. In a recent survey, we investigated COVID-19 booster vaccine willingness among Danes, a population with a high acceptance of the initial round of COVID-19 vaccination.

METHODS: The data were derived from the sixth wave of the longitudinal COVID-19 Consequences Denmark Panel Survey 2020, which included questions on booster vaccine willingness. The data from the respondents were primarily analysed using descriptive statistics. The association between age, gender, level of education, region, type of received vaccine and booster vaccine willingness was analysed using multivariate logistic regression.

RESULTS: Among those reporting to be vaccinated against COVID-19 or that their vaccination was scheduled, a weighted total of 90% indicated that they were willing to receive the booster vaccine, if/once offered. The only characteristic associated with booster vaccine willingness at the set level of statistical significance (0.05) was age (increased willingness with age; adjusted odds ratio per year: 1.04, 95% confidence interval: 1.02-1.06).

CONCLUSIONS: The willingness to receive a booster dose of a COVID-19 vaccine is high among Danes. If health authorities decide to offer COVID-19 booster vaccines to the young, they may consider providing specific information targeted at this population to increase uptake.

FUNDING: The Novo Nordisk Foundation.

TRIAL REGISTRATION: not relevant.

PMID:34913428

Scand J Rheumatol. 2021 Dec 16:1-9. doi: 10.1080/03009742.2021.1957557. Online ahead of print.

ABSTRACT

Objective: To investigate the incidence and prevalence of rheumatoid arthritis (RA) in the adult Danish population.Method: In this nationwide register-based cohort study, patients with incident RA between 1998 and the end of 2018 were identified using Danish administrative registries. The age- and sex-standardized incidence rate (IR), incidence proportion (IP), lifetime risk (LR), and point prevalence (PP) of RA were calculated. RA was defined as a first-time RA diagnosis registered in the Danish National Patient Registry combined with a redeemed prescription of a conventional synthetic disease-modifying anti-rheumatic drug in the following year. In addition, three different case definitions of RA were explored.Results: The overall age- and sex-standardized IR of RA from 1998 to 2018 was 35.5 [95% confidence interval (CI) 35.1-35.9] per 100 000 person-years while the IP was 35.2 (95% CI 34.8-35.5) per 100 000 individuals. The IR was two-fold higher for women than for men. The LR of RA ranged from 2.3% to 3.4% for women and from 1.1% to 1.5% for men, depending on the RA case definition used. The overall PP of RA was 0.6% (95% CI 0.5-0.6%) in 2018: 0.8% (95% CI 0.7-0.8%) for women and 0.3% (95% CI 0.3-0.4%) for men. The prevalence increased about 1.5-fold from 2000 to 2018.Conclusion: The IR and PP were approximately two-fold higher for women than for men. The prevalence of RA in Denmark increased significantly from 2000 to 2018. The RA case definition had more impact on the results than the choice of denominator.

PMID:34913402 | DOI:10.1080/03009742.2021.1957557