Tag: nevin manimala

Hua Xi Kou Qiang Yi Xue Za Zhi. 2024 Apr 1;42(2):207-213. doi: 10.7518/hxkq.2024.2023282.

ABSTRACT

OBJECTIVES: To determine the optimal placement of miniscrews, this study compared adult male and female patients in terms of cortical bone density, cortical bone thickness, and available bone width in the infrazygomatic crest region.

METHODS: The cone beam computed tomography imaging data of 200 patients (20-30 years old; 100 males and 100 females) were collected. The right maxillary posterior teeth in the sagittal plane were divided into six levels from proximal to distal, and three measurement sites were positioned at vertical distances of 8, 10, and 12 mm from the cementum. Cortical bone density, cortical bone thickness, and available bone width were measured in 18 measurement sites in the infrazygomatic crest and analyzed statistically.

RESULTS: The highest cortical bone density, cortical bone thickness, and available bone width in the infrazygomatic crest in adult male and female patients were at the level of the interradicular space between the maxillary second premolar and maxillary first molar. The bone cortical density and thickness increased with vertical height, whereas the available bone width decreased with increasing vertical height. Differences were observed in cortical bone density, cortical bone thickness, and available bone width between adult male and female patients.

CONCLUSIONS: The optimal implantation sites of the micro-implant anchorages in the infrazygomatic crest were at the level of the interradicular space between the maxillary second premolar and the maxillary first molar, and the vertical height of the optimal implantation site in males was appropriately higher than that in females.

PMID:38597080 | DOI:10.7518/hxkq.2024.2023282

Hua Xi Kou Qiang Yi Xue Za Zhi. 2024 Apr 1;42(2):192-206. doi: 10.7518/hxkq.2024.2023280.

ABSTRACT

OBJECTIVES: This study aimed to explore the expression trends of innate immune cells and immune-checkpoint molecules validated by data calculation in the process of oral mucosal carcinogenesis, as well as to explore methods of suppressing oral mucosal carcinogenesis based on immunotherapy by predicting their interactions. Me-thods 1) The cancer genome atlas (TCGA) database comprehensively scores immune cells and immune-checkpoint molecules in the process of oral mucosal carcinogenesis and screens out intrinsic immune cells and immune-checkpoint molecules that interfere with tumor immune escape. 2) Clinical patient blood routine data were collected for the statistical analysis of peripheral blood immune cells during the progression of oral mucosal carcinogenesis. Immune cells in peripheral blood that may affect the progression of oral mucosal carcinogenesis were screened. 3) Immunohistochemical staining was performed on intrinsic immune cells and immune-checkpoint molecules validated based on data calculation in various stages of oral mucosal carcinogenesis. 4) Special staining was used to identify innate immune cells in various stages of oral mucosal carcinogenesis based on data-calculation verification. 5) Survival analysis was conducted on intrinsic immune cells and immune-checkpoint molecules validated based on data calculation during the process of oral mucosal carcinogenesis. The association of intrinsic immune cells and immune-checkpoint molecules with the prognosis of oral squamous cell carcinoma was verified.

RESULTS: The expression of monocytes and neutrophils increased during the process of oral mucosal carcinogenesis. The expression of eosinophils showed a single peak trend of up and down. The expression of mast cells decreased. In the process of oral mucosal carcinogenesis, the expression of the immune-checkpoint molecules cytotoxic T-lymphocyte-associated protein 4 (CTLA4) and programmed cell death-ligand (PD-L1) increased. The expression trends of monocytes, neutrophils, and eosinophils were positively correlated with those of CTLA4 and PD-L1 immune-checkpoint molecules. The expression trend of mast cells was negatively correlated with the expression of CTLA4 and PD-L1. Monocytes, neutrophils, and eosinophils may promote tumor immune escape mediated by CTLA4 and/or PD-L1, thereby accelerating the progression of oral mucosal carcinogenesis. Mast cells may inhibit tumor immune escape mediated by CTLA4 and/or PD-L1, delaying the progression of oral mucosal carcinogenesis.

CONCLUSIONS: Therefore, interference with specific immune cells in innate immunity can regulate the expression of CTLA4 and/or PD-L1 to a certain extent, inhibit tumor immune escape, and delay the progression of oral mucosal carcinogenesis.

PMID:38597079 | DOI:10.7518/hxkq.2024.2023280

Hua Xi Kou Qiang Yi Xue Za Zhi. 2024 Apr 1;42(2):163-171. doi: 10.7518/hxkq.2024.2023245.

ABSTRACT

OBJECTIVES: To investigate the mechanism of circadian clock protein Bmal1 (Bmal1) on renal injury with chronic periodontitis, we established an experimental rat periodontitis model.

METHODS: Twelve male Wistar rats were randomly divided into control and periodontitis groups (n=6, each group). The first maxillary molars on both sides of the upper jaw of rats with periodontitis were ligated by using orthodontic ligature wires, whereas the control group received no intervention measures. After 8 weeks, clinical periodontal parameters, including probing depth, bleeding index, and tooth mobility, were evaluated in both groups. Micro-CT scanning and three-dimensional image reconstruction were performed on the maxillary bones of the rats for the assessment of alveolar bone resorption. Histopatholo-gical observations of periodontal and renal tissues were conducted using hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining. Renal function indicators, such as creatinine, albumin, and blood urea nitrogen levels, and oxidative stress markers, including superoxide dismutase, glutathione, and malondialdehyde levels, were measured using biochemical assay kits. MitoSOX red staining was used to detect reactive oxygen species (ROS) content in the kidneys. The gene and protein expression levels of Bmal1, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in rat renal tissues were assessed using real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical staining.

RESULTS: Micro-CT and HE staining results showed significant bone resorption and attachment loss in the maxillary first molar region of the periodontitis group. Histological examination through HE and PAS staining revealed substantial histopathological damage to the renal tissues of the rats in the periodontitis group. The findings of the assessment of renal function and oxidative stress markers indicated that the periodontitis group exhibited abnormal levels of oxidative stress, whereas the renal function levels showed abnormalities without statistical significance. MitoSOX Red staining results showed that the content of ROS in the renal tissue of the periodontitis group was significantly higher than that of the control group, and RT-qPCR and immunohistochemistry results showed that the expression levels of Bmal1, Nrf2, and HO-1 in the renal tissues of the rats in the periodontitis group showed a decreasing trend.

CONCLUSIONS: Circadian clock protein Bmal1 plays an important role in the oxidative damage process involved in the renal of rats with periodontitis.

PMID:38597076 | DOI:10.7518/hxkq.2024.2023245

Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Mar 25;40(2):225-231. doi: 10.7518/hxkq.2022.02.015.

ABSTRACT

OBJECTIVES: To evaluate the susceptibility of Streptococcus mutans (S. mutans) to NaF and the effects of Candida albicans (C. albicans) metabolites on the metabolic activity and drug resistance of S. mutans.

METHODS: The minimum inhibitory concentration (MIC) and MIC based on metabolic activity (MIC-MA) of NaF against S. mutans were measured through the broth dilution test and D₂O-labeled single-cell Raman microspectroscopy, respectively, to explore the inhibitory effect of NaF on the growth and metabolism of S. mutans. The effects of different concentrations of C. albicans supernatant on the metabolic activity of S. mutans were evaluated by using D₂O-labeled single-cell Raman microspectroscopy. The correlation between C. albicans and S. mutans was explored throughassessing the drug resistance of S. mutans against NaF in a mixed system containing C. albicans supernatant by using D₂O-labeled single-cell Raman microspectroscopy.

RESULTS: The MIC and MIC-MA of NaF against S. mutans were 0.4 g·L^-1 and 1.2 g·L^-1, respectively. At the MIC level, S. mutans showed completely inhibited growth but retained high metabolic activity. Therefore, S. mutans may regain its virulence. At the MIC-MA level, the metabolic activity of S. mutans was inhibited only when the NaF concentration reached 3×MIC. At the concentration of OD₆₀₀≥0.5, the C. albicans supernatant significantly promoted the metabolic activity of S. mutans at different time points (P<0.05). In the mixed system, the susceptibility of S. mutans to NaF decreased, and statistical differences at different time points were observed (P<0.05). The metabolic activity of S. mutans was inhibited completely only at the concentration of 4×MIC (1.6 g·L^-1).

CONCLUSIONS: D₂O-labeled single-cell Raman microspectroscopy is suitable for evaluating the effects of drugs on bacterial metabolic activity. In the mixed system, the metabolites in the C. albicans supernatant at the concentration of OD₆₀₀≥0.5 significantly promoted the metabolic activity of S. mutans and reducedthe susceptibility of S. mutans to NaF.

PMID:38597057 | DOI:10.7518/hxkq.2022.02.015

Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Mar 25;40(2):204-209. doi: 10.7518/hxkq.2022.02.012.

ABSTRACT

OBJECTIVES: This study aimed to assess the efficacy and safety of facial artery musculomucosal (FAMM) flap for small-medium tongue or floor of mouth defects caused by surgical resection of early-medium stage tongue or floor of mouth cancer.

METHODS: A retrospective cohort study was conducted and included patients with early-medium stage tongue or floor of mouth cancer and reconstructed by FAMM flap or traditional free or axial flaps. Demographic data and surgery-related data were collected. Patients were followed up for 6 months and evaluated with satisfaction, maximal mouth opening, satisfactory contour and speech, and oral intake function at months 3 and 6.

RESULTS: Forty-five patients were included, with 15 in the FAMM group and 30 in the flap group. All patients finished 3 months follow-up, and 1 in each group was lost to follow-up at month 6. All followed-up patients had no recurrence or metastasis. The FAMM group had a significantly shorter surgical time than the flap group (P<0.05). The flap group had significantly more donor sites that were uncomfortable compared with the FAMM group (P<0.05). There was no statistical significance on satisfaction, but the FAMM group had better outcomes on contour, speech, and oral intake function at month 6 than the flap group (P<0.05). The FAMM group had smaller maximal mouth opening than the flap group (P<0.05) at month 3 but equivalent maximal mouth opening at month 6 (P>0.05).

CONCLUSIONS: FAMM flap has some advantages for small-medium tongue or floor of mouth defects caused by surgical resection of early-medium stage tongue or floor of mouth cancer, and it could be an ideal choice for clinical application.

PMID:38597054 | DOI:10.7518/hxkq.2022.02.012

Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Mar 25;40(2):183-188. doi: 10.7518/hxkq.2022.02.009.

ABSTRACT

OBJECTIVES: Andrews six elements was used to explore the possible influences of gender, extraction/non-extraction, and different bracket types on the evaluation of orthodontic treatment outcome.

METHODS: Digital data from 320 selected orthodontic cases in Kunming Medical University Affiliated Stomatological Hospital during the period of 2016-2020 were divided into groups in accordance with gender, tooth extraction, and type of orthodontic brackets (DamonQ self-ligating brackets and MBT brackets). Measurement was performed using Andrews six elements as standard. The mean and standard deviation of each individual score and total score for different groups were calculated and statistically tested.

RESULTS: The differences in total scores between female and male cases exhibited no statistical significance (P>0.05). By contrast, the scores in skeletal width change of the maxilla between extraction cases and non-extraction cases presented statistical significance (P<0.05). Moreover, the scores in the anteroposterior change in the position of the maxillary incisors, the bucco-lingual border change of the mandibular arch, and the skeletal width change of the maxilla between the DamonQ self-ligating bracket and MBT bracket cases presented statistical significance (P<0.05). The cases with DamonQ self-ligating brackets had significantly lower scores than the cases with MBT brackets in the bucco-lingual border change of the mandibular arch, the skeletal width change of the maxilla, and the anteroposterior change in position of the maxillary incisors.

CONCLUSIONS: Digital 3D models can be used in the clinical retrospective studies of patients after orthodontic treatment. These models can be measured repeatedly and have clinical application value. For cases with incomplete orthodontic treatment, the control of the anteroposterior position of the incisor and the width of the arch of the upper tooth is crucial. Total treatment efficiency between female and male cases exhibited no difference, but the skeletal width change of the maxilla between extraction and non-extraction cases may exert an effect on cases with complete orthodontic treatment. The group that used DamonQ self-ligating brackets may achieve good adjustment of the bucco-lingual border change of the arch.

PMID:38597051 | DOI:10.7518/hxkq.2022.02.009

Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Mar 25;40(2):162-168. doi: 10.7518/hxkq.2022.02.006.

ABSTRACT

OBJECTIVES: To construct the three-dimensional structure of the isolated teeth of patients with dentinogenesis imperfecta type Ⅱ (DGI-Ⅱ) and dentin dysplasia type Ⅰ (DD-Ⅰ) by using Micro-CT and explore internal structure and hard tissue mineralization density.

METHODS: The three-dimensional structures of the third molars collected from patients with DGI-Ⅱ and DD-Ⅰ and healthy individuals of the same age were reconstructed by using Micro-CT (Mimics 17.0). The internal structures of the affected teeth along the sagittal and transverse planes were observed. The grayscale values of the enamel, crown dentin, and root dentin were calculated. Then, the mineralization densities of the different parts of the teeth of the three groups were analyzed.

RESULTS: The detailed three-dimensional models of the mandibular third molars with hereditary dentin defects were successfully constructed. The models contained the models of the enamel cap, dentin core, and pulp cavity. Sagittal and transverse section scans revealed that in patients with DGI-Ⅱ, the pulp cavity was incompletely calcified and the root canal was narrow, whereas in those with DD-Ⅰ, the pulp cavity and root canal were obliterated and the root of the tooth was absent. The analysis of the grayscale values showed that compared with those in the healthy group, the grayscale values of the enamel, crown dentin, and root dentin were lower in the DGI-Ⅱ and DD-Ⅰ groups (P<0.01). No significant differences in the grayscale values of the enamel and crown dentin were found between the DGI-Ⅱ and DD-Ⅰ groups (P>0.05), whereas the grayscale value of the root dentin showed statistically significant differences between the two groups (P<0.01).

CONCLUSIONS: The application of Micro-CT provided a simple and accurate method for the three-dimensional structure reconstruction and quantitative analysis of the mineralization density of isolated teeth with hereditary dentin defects. Although the dentin mineralization density of DGI-Ⅱ and DD-Ⅰ teeth decreased, the decrement shown by DD-Ⅰ teeth was more significant than that shown by DGI-Ⅱ teeth. The pulp cavity had abnormal calcifications, and the root canal was narrow or even occluded.

PMID:38597048 | DOI:10.7518/hxkq.2022.02.006

Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Mar 25;40(2):148-154. doi: 10.7518/hxkq.2022.02.004.

ABSTRACT

OBJECTIVES: This study aims to investigate the effect of X-box binding protein 1 (XBP1), a key signal molecule of ERS, on the insulin signaling pathway in adipocytes stimulated by Porphyromonas gingivalis (P. gingivalis)-lipopolysaccharide (LPS), a pathogenic bacterium of periodontitis.

METHODS: Primary cultured rat adipocytes were stimulated by P. gingivalis-LPS (100 ng·mL^-1) for 4, 8, 12, and 24 h. The protein expression levels of insulin receptor substrate-1 (IRS-1), phosphoinositide dependent protein kinase (p-PDK-1), and protein kinase B (p-AKT-1) in the insulin signaling pathway were detected by Western blot analysis. pLVX-NC1, pLVX-XBP1, pLVX-NC2, and pLVX-XBP1-RNAi were transfected into adipocytes, respectively. The transfected rat adipocytes were stimulated by P. gingivalis-LPS, and the protein expression of the insulin signaling pathway was detected by Western blot.

RESULTS: The Western Blot showed decreased protein expression of the insulin signaling pathway in rat adipocytes stimulated with P. gingivalis-LPS compared with the control, and the difference was statistically significant (P<0.05). The protein expression levels of IRS-1, p-PDK-1, and p-AKT in the rat adipocytes of pLVX-XBP1 were significantly higher than those in pLVX-NC1 at 8 and 12 h after P. gingivalis-LPS stimulation (P<0.05). The protein expression levels of IRS-1, p-PDK-1, and p-AKT in the rat adipocytes of pLVX-XBP1-RNAi were significantly lower than those in pLVX-NC2 at 4, 8, 12, and 24 h after P. gingivalis-LPS stimulation (P<0.05).

CONCLUSIONS: P. gingivalis-LPS regulates the insulin signaling pathway in adipocytes th-rough XBP1.

PMID:38597046 | DOI:10.7518/hxkq.2022.02.004

Hua Xi Kou Qiang Yi Xue Za Zhi. 2023 Dec 1;41(6):708-712. doi: 10.7518/hxkq.2023.2023204.

ABSTRACT

OBJECTIVES: A retrospective study was conducted on the effect of primary rhinoplasty on infants with unilateral complete cleft lip nasal deformity.

METHODS: Infants with unilateral complete cleft lip in the Department of Cleft Lip and Palate Surgery, College of Stomatology, Xi’an Jiaotong University were selected. All infants underwent cheiloplasty and primary rhinoplasty. We reconstructed the nasal base and corrected the nasal septum and alar deformity at the same time. The nasal splint was worn 1 week after the surgery. The nasal morphology before surgery as well as 1 week and 1 year after surgery were analyzed.

RESULTS: Significant differences were found on symmetry ratios including nasal base width, nostril height, alar angle and columella deviation angle between before and after operation (P<0.05). There were statistically significant differences in the symmetry ratio of nostril height and columella deviation angle between 1 year after surgery and 1 week after surgery (P<0.05).

CONCLUSIONS: Infants with unilateral complete cleft lip nasal deformity can achieve satisfactory nasal morphology by primary rhinoplasty. Despite few cases of recurrence of nasal deformity, the nasal morphology can be well improved and maintained.

PMID:38597037 | DOI:10.7518/hxkq.2023.2023204

Hua Xi Kou Qiang Yi Xue Za Zhi. 2023 Dec 1;41(6):671-677. doi: 10.7518/hxkq.2023.2023110.

ABSTRACT

OBJECTIVES: This study aims to analyze and summarize the characteristics of supernumerary teeth by using cone-beam computed tomography (CBCT).

METHODS: A total of 718 patients with 1 138 supernumerary teeth were retrospectively collected. Age, gender, number, location, morphology, eruption status, and accompanying symptoms of the supernumerary teeth were statistically analyzed. The relationship relative to jaws, gender, and eruption status were analyzed and discussed.

RESULTS: The average age of the patients was 9.54±5.32 years, and the male to female ratio was 2.88∶1. About 77.02% of the patients sought medical advice during the mixed dentition period, and 50.70% had one supernumerary tooth. These supernumeraries were most commonly conical in shape, and 85.76% of them were in the incisor region, 92.09% in the upper jaw, 46.75% in inverted position, and 86.20% unerupted. Overall, 65.29% of them had fully developed roots, and 60.63% had an impact on adjacent structures. Significant differences were found in eruption status, morphology, zoning, direction, root development, and impact on adjacent structures between the supernumerary teeth located in the upper and lower jaws (P<0.05). Significant differences were also detected in gender, morphology, zoning, orientation, root development, and impact on adjacent structures between erupted and unerupted teeth (P<0.05). The incidence of supernumerary teeth in the incisor region was higher in males than that in females. Moreover, the root of supernumeraries was more completely developed in males than in females (P<0.05).

CONCLUSIONS: For supernumerary teeth, CBCT images can provide accurate three-dimensional radiographic data and are valuable for clinical diagnosis and treatment planning.

PMID:38597032 | DOI:10.7518/hxkq.2023.2023110