Tag: pubmed

CNS Neurosci Ther. 2025 Aug;31(8):e70529. doi: 10.1111/cns.70529.

ABSTRACT

AIMS: Postoperative sleep disturbance (PSD) is a common complication following surgical procedures. We aimed to evaluate the effect of prefrontal transcranial alternating current stimulation (tACS) in preventing PSD among patients undergoing gynecological laparoscopic surgery.

METHODS: A total of 176 eligible patients, aged 18-65 years, with ASA Class I to III, and scheduled for gynecological laparoscopic surgery, were randomly allocated to receive either a single 20-min session of prefrontal tACS (2 mA, 7 Hz) or sham stimulation immediately after extubation. The primary outcome was the occurrence of PSD on postoperative day (POD) 1.

RESULTS: The intention-to-treat analysis showed a statistically significant reduction in PSD incidence on POD 1 in the active tACS group (23.9%) compared to the sham group (43.2%), with an odds ratio of 0.41 (95% CI, 0.22-0.79; p = 0.007). Additionally, patients in the active tACS group reported significantly lower anxiety scores on POD 1 (p < 0.001), while depression scores were comparable between the groups. The active tACS group also reported significantly lower pain scores, both on PODs 1 (movement: p = 0.002; rest: p < 0.001) and 3 (movement: p = 0.028; rest: p < 0.001).

CONCLUSIONS: A single session of prefrontal tACS significantly reduces the incidence of PSD on POD 1 and may offer additional benefits in reducing early postoperative anxiety and pain, with a favorable safety and tolerability profile.

TRIAL REGISTRATION: China Clinical Trial Registration Center: ChiCTR2300078658.

PMID:40755300 | DOI:10.1111/cns.70529

Brief Bioinform. 2025 Jul 2;26(4):bbaf384. doi: 10.1093/bib/bbaf384.

ABSTRACT

BACKGROUND: The COVID-19 pandemic has caused over 776 million infections and 7 million deaths globally between December 2019 and November 2024. Since the emergence of the original Wuhan strain, SARS-CoV-2 has evolved into multiple variants-including Alpha, Delta, and Omicron-primarily through mutations in the Spike glycoprotein. The S1 subunit, which binds the human angiotensin-converting enzyme 2 (ACE2) receptor, mutates frequently and plays a key role in infectivity and immune escape, while the more conserved S2 subunit mediates membrane fusion. Anticipating future mutations is essential for guiding vaccine design and therapeutic strategies. Generative Large Language Models (LLMs) have shown promise in protein sequence modeling due to their capacity to produce realistic and functional synthetic sequences. Here, we introduce SARITA, a GPT-3-based LLM with up to 1.2 billion parameters, fine-tuned via continual learning on the protein model RITA trained on 107 017 high-quality SARS-CoV-2 Spike sequences (up to March 1st 2021) to generate high-quality synthetic SARS-CoV-2 Spike S1 subunits.

RESULTS: SARITA is able to generate realistic, full-length synthetic S1 subunits starting from a 14-amino-acid prompt. When evaluated on unseen sequences collected between March 2021 and November 2023-including major Variants of Concern (VOCs) such as Delta and Omicron, and Variants of Interest such as Iota-SARITA outperforms baseline and state-of-the-art LLMs in terms of sequence quality, biological plausibility, and similarity to real-world viral evolution. SARITA generates high-quality sequences in over 97% of cases, with markedly lower False Mutation Rate and higher similarity scores (PAM30, Levenshtein distance) compared to alternative approaches. It also accurately reproduces key mutations characteristic of future variants-such as L212I, R158L, T95P, and E406K-which were not present in the training data but emerged later in VOCs like Omicron and Delta. Structure-based analysis confirms the functional plausibility of these substitutions, with ΔΔG values within experimentally supported thresholds for ACE2 and antibody binding. Furthermore, SARITA anticipates immune-evasive mutations and accurately captures the positional and statistical distribution of mutations found in post- March 1st 2021 variants, highlighting its potential as a predictive tool for viral evolution.

CONCLUSION: These results indicate the potential of SARITA to predict future SARS-CoV-2 S1 evolution, potentially aiding in the development of adaptable vaccines and treatments.

PMID:40755284 | DOI:10.1093/bib/bbaf384

Nurs Open. 2025 Aug;12(8):e70288. doi: 10.1002/nop2.70288.

ABSTRACT

AIMS: The aim of this study is to determine the psychological resilience and self-efficacy levels of caregivers of individuals with Alzheimer’s disease and to examine the effects of these variables on the caregiver burden.

DESIGN: A cross-sectional, quantitative design was used.

METHODS: The study was conducted in the neurology outpatient clinic of a university hospital between March and July 2022. The sample of the study consisted of caregivers of 105 individuals diagnosed with Alzheimer’s disease. Data were collected using the “Zarit Burden Interview,” the “Revised Scale for Caregiving Self-Efficacy,” and the “Resilience Scale Short Form.” In the study, descriptive statistics and Pearson correlation analysis were employed, and the stepwise multiple regression model was applied.

RESULTS: It was determined that caregivers had a moderate to severe caregiving burden (43.57 ± 16.58) and a moderate level of psychological resilience (20.24 ± 5.96) and self-efficacy (62.84 ± 15.96). The stepwise regression analysis results showed that a lower mini mental state score (β = -0.821; p < 0.001), low caregiver income status (β = -0.201; p < 0.008), inadequate social support (β = -0.155; p < 0.030), shorter duration of Alzheimer’s disease diagnosis (β = -0.323; p < 0.001), advanced age (β = -0.299; p < 0.001), and prolonged caregiving (β = -0.216; p < 0.005) were the main predictors of caregiver burden. In addition, the levels of caregiver’s self-efficacy in obtaining respite (β = -0.340; p < 0.001), psychological resilience (β = -0.293; p < 0.001), and self-efficacy in controlling upsetting thoughts (β = -0.240; p < 0.001) about caregiving predicted caregiver burden significantly.

CONCLUSION: The resilience and self-efficacy levels of caregivers predicted caregiver burden significantly. As the psychological resilience and self-efficacy levels of caregivers increase, caregiver burden may decrease significantly.

PATIENT OR PUBLIC CONTRIBUTION: PATIENT OR PUBLIC CONTRIBUTION: Interviews were conducted with the research participants to collect data. Our findings can be used to develop interventions and coping strategies to reduce the care burden of caregivers of individuals with Alzheimer’s disease and to improve their resilience and self-efficacy.

REPORTING METHOD: The results were reported in accordance with STROBE guidelines.

PMID:40755264 | DOI:10.1002/nop2.70288

Disaster Med Public Health Prep. 2025 Aug 4;19:e215. doi: 10.1017/dmp.2025.10084.

ABSTRACT

OBJECTIVES: The aim was to characterize reported food- and waterborne outbreaks in Finland, 2010-2020, and to test local investigation teams’ preparedness to investigate outbreaks.

METHODS: The outbreaks reported to the Finnish registry for food and waterborne outbreaks were characterized by the number of outbreaks and people fallen ill, and the causative agent. Local investigation teams’ measures and their timeliness in a simulated time-constrained case study were scored and analyzed descriptively.

RESULTS: In 537 outbreaks, 12 399 fell ill and 19 (0.15%) died. The causative agent remained unknown in 218 outbreaks. The local investigation teams’ median preparedness score was 15/29 (range 9-23) and the score differed markedly within regions. Differences in the speed of communication and the number of channels used were observed between the teams.

CONCLUSIONS: Differences between environmental health units’ scores indicated inconsistency in outbreak investigations between areas in Finland. The variability in preparedness scores was high in both the highest and lowest outbreak incidence regions. Because outbreaks occur rarely in most EHU areas, preparedness exercises are necessary to maintain investigation skills. Measures to enhance sampling would be needed because the causative agent was unknown in over 1/3 of the outbreaks. Many local investigation teams lack experience in public communication and training on communicating about outbreaks is needed.

PMID:40755261 | DOI:10.1017/dmp.2025.10084

Thorac Cancer. 2025 Aug;16(15):e70144. doi: 10.1111/1759-7714.70144.

ABSTRACT

BACKGROUND: While established biomarkers predict immunotherapy response in advanced nonsmall cell lung cancer (NSCLC), additional noninvasive imaging biomarkers may enhance treatment selection. Pretreatment computed tomography (CT) texture analysis may provide tumor characterization to predict survival outcomes.

METHODS: We conducted a systematic review and meta-analysis following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. PubMed and Cochrane Library databases were searched. Study quality was assessed using the quality in prognosis studies (QUIPS) tool. Hazard ratios (HRs) with 95% confidence intervals (CIs) were pooled using random-effects models.

RESULTS: Ten retrospective studies involving 2400 patients were included. Patients stratified as low-risk based on CT texture features demonstrated significantly improved survival outcomes compared to high-risk patients. The included studies used diverse radiomic features for risk stratification, including texture features from gray-level co-occurrence matrix (GLCM) such as entropy and dissimilarity, first-order statistical parameters including skewness and kurtosis, gray-level run-length matrix (GLRLM) features, and deep learning-derived features. Meta-analysis of five studies (n = 1102) revealed that patients stratified as low-risk based on these quantitative CT texture signatures had substantially better overall survival (OS) (p < 0.0001) with minimal heterogeneity (I² = 0.0%). Similarly, progression-free survival (PFS) analysis of five studies (n = 1799) showed significant benefit for low-risk patients (p < 0.0001), though with moderate heterogeneity (I² = 71.7%).

CONCLUSIONS: Pretreatment quantitative CT texture analysis effectively predicts survival outcomes in advanced NSCLC patients receiving immunotherapy, providing clinically meaningful risk stratification. This noninvasive imaging approach may serve as an additional tool to complement established pathological and molecular biomarkers, including liquid biopsy, for enhanced personalized treatment selection.

PMID:40755255 | DOI:10.1111/1759-7714.70144

Ren Fail. 2025 Dec;47(1):2536194. doi: 10.1080/0886022X.2025.2536194. Epub 2025 Aug 4.

ABSTRACT

BACKGROUND: The appropriate concentration of dialysate calcium (DCa) for maintenance hemodialysis (MHD) patients remains a subject of ongoing debate. The relationship between DCa concentration and patient outcomes is not yet well established. This study aimed to evaluate the impact of DCa concentration on mortality and major adverse cardiovascular and cerebrovascular events (MACCEs) in Chinese patients undergoing MHD.

METHODS: A retrospective matched cohort study was conducted, analyzing data from hemodialysis (HD) patients at our center over the past five years. Each patient in the low DCa group was matched with a counterpart from the mid DCa group. Outcomes, including mortality, MACCE rates, fracture rates, and hospitalization rates, were compared between the two groups.

RESULTS: The study included 924 MHD patients, of whom 101 low DCa patients were matched with 101 mid DCa patients. In the matched-pair analysis, no significant difference was observed in all-cause mortality between the low DCa and mid DCa groups (3.5/100 vs. 4.7/100 patient-years). However, the low DCa group exhibited lower cumulative rates of MACCEs, hospitalization, and fracture incidence compared with the mid DCa group (7.3/100 vs. 20.7/100 patient-years, 21.5/100 vs. 34/100 patient-years, and 1.6/100 vs. 3.9/100 patient-years, respectively). In addition, the adjusted hazard ratio (HR) for the occurrence of first MACCE in the low DCa group compared with the mid DCa group was 0.47 (95% CI: 0.24-0.91).

CONCLUSIONS: These findings suggest that low DCa concentrations are associated with reduced rates of MACCEs, hospitalization, and cumulative fracture incidence in MHD patients relative to mid DCa concentrations.

PMID:40755245 | DOI:10.1080/0886022X.2025.2536194

Appl Neuropsychol Adult. 2025 Aug 4:1-7. doi: 10.1080/23279095.2025.2540586. Online ahead of print.

ABSTRACT

Gamma transcranial alternating current stimulation (Gamma-tACS) is used for treating Alzheimer’s disease (AD). The changes in the Gamma band power (GBP) of Electroencephalogram (EEG) manifested by AD can justify the reliability of enhancing the GBP via the Gamma-tACS. The EEG is the electric potential originating from the neural activity of the human brain. Our objective is to statistically investigate how far the AD patients differ from the healthy subjects in terms of GBP of resting-state EEG at the frontal electrodes. The dataset contains EEG recordings from 65 participants comprising 36 AD patients with an age of 66.39 ± 7.89 and 29 healthy subjects with an age of 67.90 ± 5.40. As a major finding, the Wilcoxon rank-sum test reveals that the difference in GBP of AD patients and healthy subjects is not significant at frontal polar 1 (FP1) (p = 0.81), FP2 (p = 0.48), frontal 3 (F3) (p = 0.77), F7 (p = 0.76), frontal zone (Fz) (p = 0.65), F4 (p = 0.91), and F8 (p = 0.42).

PMID:40755242 | DOI:10.1080/23279095.2025.2540586

Ann Med. 2025 Dec;57(1):2542439. doi: 10.1080/07853890.2025.2542439. Epub 2025 Aug 4.

ABSTRACT

OBJECTIVE: To systematically evaluate the impact of antithrombin III (AT III) supplementation on the prognosis of patients undergoing extracorporeal membrane oxygenation (ECMO).

MATERIALS AND METHODS: A comprehensive literature search was conducted in PubMed, Web of Science, Embase, and the Cochrane Library for studies assessing the effects of AT III supplementation on ECMO patient outcomes. The risk of bias was assessed using the Cochrane Risk of Bias and The Newcastle-Ottawa Scale.

RESULTS: A total of six studies involving 18,641 ECMO-treated patients were included. The meta-analysis showed that AT III supplementation did not reduce mortality in ECMO patients (RR = 1.17, 95% CI: 0.85-1.60, p = 0.34) and had no significant benefit in reducing bleeding events (RR = 1.04, 95% CI: 0.90-1.21, p = 0.56) or thrombosis (RR = 1.29, 95% CI: 0.81-2.05, p = 0.29). Subgroup analysis revealed that in cardiac ECMO patients, AT III supplementation was associated with an increased mortality but a reduced risk of thromboembolism. Conversely, in other ECMO support types, AT III supplementation was linked to a higher incidence of thromboembolism, with adult patients also showing an increased thromboembolism rate. No statistically significant differences were observed in other subgroup analyses.

CONCLUSION: Overall, AT III supplementation does not reduce in-hospital mortality, bleeding, or thrombotic complications in ECMO patients and may even pose risks in certain populations. Therefore, routine AT III supplementation in ECMO patients may be not currently recommended.

PMID:40755240 | DOI:10.1080/07853890.2025.2542439

J Food Sci. 2025 Aug;90(8):e70450. doi: 10.1111/1750-3841.70450.

ABSTRACT

Long-term glucocorticoid therapy is known to induce osteoporosis by disrupting bone metabolism and altering gut microbiota. In this study, C57BL/6J mice were used to establish a glucocorticoid-induced osteoporosis (GIOP) model by intramuscular injection of dexamethasone. The mice were then divided into control, GIOP, and experimental groups; the experimental group was orally administered selenium-enriched egg white protein (Se-EWP) in order to evaluate its bone-protective effects and potential mechanisms. The results showed that Se-EWP significantly improved bone strength, trabecular microstructure, and bone mineral density in GIOP mice; enhanced the expression of bone synthesis-related proteins, such as osterix and osteocalcin; and decreased the expression of bone catabolism-related proteins, such as tartrate-resistant acid phosphatase, receptor activator of nuclear factor-κB ligand, and cathepsin K. In addition, Se-EWP was found to alleviate glucocorticoid-induced gut microflora dysbiosis by increasing beneficial bacteria and microbial diversity. Se-EWP significantly modulated the 5-hydroxytryptamine receptor 1B (Htr1b) pathway, which is crucial for bone homeostasis and gut-bone communication. The modulation of the Htr1b pathway suggests that Se-EWP may exert its anti-osteoporotic effects through this mechanism. These effects were statistically significant (p < 0.05). Correlation analysis revealed significant associations between changes in the intestinal microbiota and bone metabolism parameters, further supporting the hypothesis that Se-EWP exerts its effects through the gut-bone axis. This suggests a novel therapeutic mechanism of action for Se-EWP through the gut-bone axis. In conclusion, this study provides strong evidence supporting the application of Se-EWP in the prevention and treatment of GIOP.

PMID:40754656 | DOI:10.1111/1750-3841.70450

Phytother Res. 2025 Aug 3. doi: 10.1002/ptr.70058. Online ahead of print.

ABSTRACT

Flavonoids remain controversial regarding their role in cancer-related mortality and all-cause mortality in cancer patients. A comprehensive search was conducted on Web of Science, PubMed, and CINAHL up to February 2024. Using adjusted hazard ratios (HRs), we compared flavonoid intake in the highest with lowest categories. Random-effects models were employed when the I² statistic surpassed 50%, while fixed-effects models were utilized otherwise. Fifteen eligible articles, comprising 19 cohorts, met the inclusion criteria. A significant inverse association was observed between total flavonoid intake and all-cause mortality (HR: 0.95, 95% CI: 0.91-0.99). However, the study found no significant association between total flavonoid intake and reduced cancer-related mortality risk (HR: 0.93, 95% CI: 0.83-1.04). A meta-regression analysis showed that the hazard ratio significantly decreased with the duration of follow-up (p = 0.005). Among flavonoid subclasses, flavan-3-ols intake was linked to a reduced risk of cancer-related mortality (HR: 0.74, 95% CI: 0.59-0.94). The consumption of flavanones, flavones, and isoflavones was correlated with a reduced likelihood of mortality from any cause, with summary HRs of 0.97 (95% CI: 0.95-0.99), 0.95 (95% CI: 0.92-0.98), and 0.88 (95% CI: 0.80-0.97), respectively. This study indicated that dietary intake of total flavonoids, flavanones, flavones, and isoflavones is inversely correlated with the risk of all-cause mortality in cancer patients.

PMID:40754648 | DOI:10.1002/ptr.70058