Tag: pubmed

J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):424. doi: 10.4103/jomfp.jomfp_146_22. Epub 2023 Jul 13.

ABSTRACT

BACKGROUND: Hematoxylin and eosin staining is the globally accepted staining technique for histology and histopathology sections. Hematoxylin is a natural dye as compared to eosin which is a synthetic dye manufactured from chemicals. Eosin acts as a counterstain to hematoxylin giving a sharp contrast to its blue color. Eosin being a chemical, can cause health hazards and environmental pollution. Using eco-friendly materials and going organic is the demand of this era. Curcuma longa rhizomes are proven to contain curcuminoids that exhibit various medicinal properties and curcumin the bright yellow component of the plant, that is, being used as a colorant. This natural dye from C. longa provides a significant alternative to eosin as they are safer to use without health hazards and are biodegradable and have easy disposability.

AIM: To evaluate and compare the staining qualities of C. longa (turmeric) with hematoxylin and eosin (H&E).

MATERIALS AND METHODS: A total of 100 paraffin-embedded tissue block specimens of various pathological tissues were stained with H and E and hematoxylin and curcumin (H&C). Evaluation and comparison of the staining efficacy were done for all the pathological tissues. The obtained data were subjected to statistical analysis.

RESULTS: H&C staining gave comparable results with H and E staining with a statistically insignificant P value (p > 0.05).

CONCLUSION: Curcumin has shown equivalent staining to that of eosin. Thus, it can be used as a better alternative.

PMID:37854923 | PMC:PMC10581282 | DOI:10.4103/jomfp.jomfp_146_22

J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):402-405. doi: 10.4103/jomfp.jomfp_205_22. Epub 2023 Jul 13.

ABSTRACT

INTRODUCTION: Age estimation using regressive alterations such as root dentin translucency, PDL attachment, and attrition are easy and reliable way of predicting the age. However, extensive and population-specific formula has not been generated. This attempt was to assess the correlation of these alterations with age and to generate a Linear regressive formula, specific to this population.

METHODS AND MATERIAL: Three alterations were assessed such as dental attrition, root dentin translucency, and periodontal attachment level from the extracted teeth. Dental attrition was measured using Johanson’s and Li and Ji criteria. PDL attachment level and root dentin translucency was measured using the Johanson method and the Lamendin method.

STATISTICAL ANALYSIS: SPSS software (Version 27), Pearson correlation test, and Linear regressive analysis were used.

RESULTS: Our results showed all three factors/parameters such as attrition, periodontal ligament, and translucency having a very good correlation with age and correlation coefficient r value ranging from 0.6 to 0.8. All the parameters were having statistically significant correlation with P value <0.005. Among them, root dentin translucency with Johanson G method showed excellent correlation with r = 0.83 followed by PDL attachment by Johanson G method with r = 0.702.

CONCLUSIONS: Regressive changes such as Dentin translucency, PDL attachment and attrition on Coastal Karnataka showed a very good correlation with age. Among them, Dentin translucency by Johanson G method had the best correlation with the a standard error of estimate (SEE). Results of our study indicates that all these parameters [Translucency, PDL attachment, and attrition] can be utilized in age estimation.

PMID:37854921 | PMC:PMC10581294 | DOI:10.4103/jomfp.jomfp_205_22

J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):426. doi: 10.4103/jomfp.jomfp_136_22. Epub 2023 Jul 13.

ABSTRACT

BACKGROUND: Pain and anxiety are the most reported negative effect of orthodontic treatment which can influence the quality of life and are responsible for the discontinuation of treatment. These unpleasant experiences raise the stress levels reflected by the increase in the concentration of salivary alpha-amylase and nitric oxide. These can be assessed through various subjective methods which have limited value as it depends on the patient’s honesty, assessing whether using objective methods are reliable.

OBJECTIVES: To assess pain and anxiety-induced stress levels using both physiological (Salivary alpha-amylase and Salivary nitric oxide) and psychological testing instruments (Visual analog scale and State Trait Anxiety Inventory Y6) during the initial phases of fixed orthodontic treatment.

MATERIALS AND METHODS: A total of 30 patients were included. Pain and anxiety instruments were applied and saliva samples were collected from patients before and after separator placement, before and 48 h after archwire insertion and 1 month after archwire insertion.

RESULTS: The mean values of alpha-amylase (235.3600), nitric oxide levels (2.060) and pain scores (7.8667) were higher 48 h after archwire insertion (T3) which are statistically significant, P = 0.00. Anxiety scores (57.7000) were high before separator placement (T0). No statistically significant correlation was found among pain and alpha-amylase, anxiety and nitric oxide levels.

CONCLUSION: Initial alignment phases of fixed orthodontic treatment affect patients’ anxiety and stress levels. The findings could be a result of the psychological stress due to procedures in the initial phases of the treatment.

PMID:37854920 | PMC:PMC10581320 | DOI:10.4103/jomfp.jomfp_136_22

J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):333-339. doi: 10.4103/jomfp.jomfp_157_23. Epub 2023 Jul 13.

ABSTRACT

AIM: The current study evaluates and compares the percentage distribution of different microorganisms according to their strains and occurrence among the three studied groups.

METHOD AND METHODOLOGY: Sample of 30 removable dental prosthesis wearing patients was selected: wearing either complete dentures or partial dentures and without any significant medical history or on prescription medication for the past 3-6 months. Samples were obtained in three subcategories based on the duration of prostheses worn by the patient. A sterile swab made up of cotton moistened with phosphate buffer saline (PBS) was scrubbed on the dental prosthesis at the fitting surfaces and the denture-bearing area of the oral cavity. Within two hours, the collected swab sample was infused in the sterile tube containing 1 ml of 0.84% PBS solution maintained at pH 7-7.2 and sent for microbiological analysis. The samples were then inoculated into different medias. Microbial growth was checked after incubating the culture plates for 48 h at 37°C. Microorganisms were recognized and counted by calibrated colony counter. Gram’s stain was used to stain the colony smear and biochemical tests such as coagulase, catalase, oxidase, sugar fermentation with acid and gas production (triple sugar iron), methyl red test, test for indole production, hydrogen sulphide (H₂S) production, citrate utilization, urease test, germ tube tests were performed.

STATISTICAL ANALYSIS: Descriptive statistics included calculation of means and standard deviation using multivariate analysis. All values were considered statistically significant for a value of P < 0.05.

RESULTS: Streptococcus species, Coagulase-negative staphylococcus, Staphylococcus aureus, Candida albicans and Klebsiella pneumoniae showed the maximum positive culture among the secluded microorganisms in all three groups.

CONCLUSION: A progressive increase in the microbial contamination was directly proportional to the duration of removable prosthesis usage.

PMID:37854919 | PMC:PMC10581316 | DOI:10.4103/jomfp.jomfp_157_23

Integr Zool. 2023 Oct 18. doi: 10.1111/1749-4877.12770. Online ahead of print.

ABSTRACT

The dynamics of populations of small mammals of Central Siberia was analyzed. The studies were carried out at the Yenisei ecological station “Mirnoye” of the A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences. The time series analysis was performed by the wavelet transform using the statistical data processing language R. In the 20th century, the dynamics of the population of the community and some of its constituent species (Sorex araneus; S. caecutiens; S. isodon; S. tundrensis; S. minutus; Craseomys rufocanus; Clethrionomys rutilus; Microtus oeconomus; M. agrestis) were characterized by a 4-year periodicity. The type of dynamics changed to noncyclic by the nineties, but by 2022, four species (S. araneus, S. isodon, C. rutilus, and M. oeconomus) and the community as a whole showed a tendency toward recovery of population cycles. The remaining species were characterized by consistently low numbers with irregular low amplitude fluctuations.

PMID:37853517 | DOI:10.1111/1749-4877.12770

BMJ Open. 2023 Oct 18;13(10):e074700. doi: 10.1136/bmjopen-2023-074700.

ABSTRACT

INTRODUCTION: Despite technological advancements in recent years, glenoid component loosening remains a common complication after anatomical total shoulder arthroplasty (ATSA) and is one of the main causes of revision surgery. Increasing emphasis is placed on the prevention of glenoid component failure. Previous studies have successfully predicted range of motion, patient-reported outcomes and short-term complications after ATSA using machine learning methods, but an accurate predictive model for (glenoid component) revision is currently lacking. This study aims to use a large international database to accurately predict aseptic loosening of the glenoid component after ATSA using machine learning algorithms.

METHODS AND ANALYSIS: For this multicentre, retrospective study, individual patient data will be compiled from previously published studies reporting revision of ATSA. A systematic literature search will be performed in Medline (PubMed) identifying all studies reporting outcomes of ATSA. Authors will be contacted and invited to participate in the Machine Learning Consortium by sharing their anonymised databases. All databases reporting revisions after ATSA will be included, and individual patients with a follow-up less than 2 years or a fracture as the indication for ATSA will be excluded. First, features (predictive variables) will be identified using a random forest feature selection. The resulting features from the compiled database will be used to train various machine learning algorithms (stochastic gradient boosting, random forest, support vector machine, neural network and elastic-net penalised logistic regression). The developed and validated algorithms will be evaluated across discrimination (c-statistic), calibration, the Brier score and the decision curve analysis. The best-performing algorithm will be used to create an open-access online prediction tool.

ETHICS AND DISSEMINATION: Data will be collected adhering to the WHO regulation on data sharing. An Institutional Review Board review is not applicable. The study results will be published in a peer-reviewed journal.

PMID:37852772 | DOI:10.1136/bmjopen-2023-074700

BMJ Open. 2023 Oct 18;13(10):e073419. doi: 10.1136/bmjopen-2023-073419.

ABSTRACT

OBJECTIVE: This study examines trends, spatial distribution and determinants of age at death of under-5 children in Ethiopia.

DESIGN: This study used secondary data from the 2000, 2005, 2011 and 2016 Ethiopian Demographic and Health Surveys. A multilevel partial ordinal logistic regression model was used to assess the effects of variables on the age at death of children under 5 years.

SETTING: Ethiopia.

PARTICIPANTS: The final analysis included a sample of 3997 deaths of newborns, infants and toddlers.

RESULTS: A total of 1508, 1054, 830 and 605 deaths of under-5 children were recorded in the 2000, 2005, 2011 and 2016 survey years, respectively. The death of newborns, infants and toddlers showed a significant decrease from 2000 to 2016, with reductions of 33.3% to 17.4%, 42.4% to 12.6% and 45.2% to 11.6%, respectively. The analysis using Global Moran’s Index revealed significant spatial autocorrelation in mortality for each survey year (p<0.05). The intraclass correlation of age at death of under-5 children within regions was substantial. Furthermore, the odds of newborn deaths among under-5 children (OR: 0.638, 95% CI: 0.535, 0.759) were lower for those delivered in health institutions compared with those delivered at home.

CONCLUSIONS: Throughout the survey years from 2000 to 2016, newborn children had higher mortality rates compared with infants and toddlers, and significant spatial variations were observed across different zones in Ethiopia. Factors such as child’s sex, age of mother, religion, birth size, sex of household head, place of delivery, birth type, antenatal care, wealth index, spatial autocovariate, Demographic and Health Survey year, place of residence and region were found to be significant in influencing the death of under-5 children in Ethiopia. Overall, there has been a decreasing trend in the proportion of under-5 child mortality over the four survey years in Ethiopia.

PMID:37852770 | DOI:10.1136/bmjopen-2023-073419

BMJ Open. 2023 Oct 18;13(10):e076154. doi: 10.1136/bmjopen-2023-076154.

ABSTRACT

BACKGROUND: Based on 2021 data, Nigeria had the second largest number of zero-dose children globally estimated at over 2.25 million, concentrated in the northern part of the country due to factors some of which are sociocultural. This study analysed the impact of the COVID-19 pandemic and response on childhood vaccination in Northern Nigeria.

METHODS: Using a mixed methods sequential study design in the most populous northern states of Kaduna and Kano, quantitative routine immunisation data for the period 2018-2021 and qualitative data collected through 16 focus group discussions and 40 key informant interviews were used. An adaptation of the socioecological model was used as a conceptual framework. Mean vaccination coverages and test of statistical difference in childhood vaccination data were computed. Qualitative data were coded and analysed thematically.

RESULTS: Mean Penta 1 coverage declined in Kaduna from 69.88% (SD=21.02) in 2018 to 59.54% (SD=19.14%) by 2021, contrasting with Kano where mean Penta 1 coverage increased from 51.87% (SD=12.61) to 56.32% (SD=17.62%) over the same period. Outreaches and vaccination in urban areas declined for Kaduna state by 10% over the pandemic period in contrast to Kano state where it showed a marginal increase. The two states combined had an estimated 25% of the country’s zero-dose burden in 2021. Lockdowns, lack of transport and no outreaches which varied across the states were some of the factors mentioned by participants to have negatively impacted childhood vaccination. Special vaccination outreaches were among the recommendations for ensuring continued vaccination through a future pandemic.

CONCLUSION: While further interrogating the accuracy of denominator estimates for the urban population, incorporating findings into pandemic preparedness and response will ensure uninterrupted childhood vaccination during emergencies. Addressing the identified issues will be critical to achieving and sustaining universal childhood vaccination in Nigeria.

PMID:37852768 | DOI:10.1136/bmjopen-2023-076154

BMJ Open. 2023 Oct 18;13(10):e067684. doi: 10.1136/bmjopen-2022-067684.

ABSTRACT

OBJECTIVES: To estimate the economic costs, health-related quality-of-life outcomes and cost-effectiveness of subthreshold micropulse laser (SML) versus standard laser (SL) for the treatment of diabetic macular oedema (DMO) with central retinal thickness (CRT) of <400µ.

DESIGN: An economic evaluation was conducted within a pragmatic, multicentre, randomised clinical trial, DIAbetic Macular Oedema aNd Diode Subthreshold.

SETTING: 18 UK Hospital Eye Services.

PARTICIPANTS: Adults with diabetes and centre involving DMO with CRT<400µ.

INTERVENTIONS: Participants (n=266) were randomised 1:1 to receive SML or SL.

METHODS: The base-case used an intention-to-treat approach conducted from a UK National Health Service (NHS) and personal social services (PSS) perspective. Costs (2019-2020 prices) were collected prospectively over the 2-year follow-up period. A bivariate regression of costs and quality-adjusted life-years (QALYs), with multiple imputation of missing data, was conducted to estimate the incremental cost per QALY gained and the incremental net monetary benefit of SML in comparison to SL. Sensitivity analyses explored uncertainty and heterogeneity in cost-effectiveness estimates.

RESULTS: One participant in the SL arm withdrew consent for data to be used; data from the remaining 265 participants were included in analyses. Mean (SE) NHS and PSS costs over 24 months were £735.09 (£111.85) in the SML arm vs £1099.70 (£195.40) in the SL arm (p=0.107). Mean (SE) QALY estimates were 1.493 (0.024) vs 1.485 (0.020), respectively (p=0.780), giving an insignificant difference of 0.008 QALYs. The probability SML is cost-effective at a threshold of £20 000 per QALY was 76%.

CONCLUSIONS: There were no statistically significant differences in EQ-5D-5L scores or costs between SML and SL. Given these findings and the fact that SML does not burn the retina, unlike SL and has equivalent efficacy to SL, it may be preferred for the treatment of people with DMO with CRT<400µ.

TRIAL REGISTRATION NUMBERS: ISRCTN17742985; NCT03690050.

PMID:37852765 | DOI:10.1136/bmjopen-2022-067684

BMJ Open. 2023 Oct 18;13(10):e071104. doi: 10.1136/bmjopen-2022-071104.

ABSTRACT

OBJECTIVE: The study aimed to investigate the effects of education and age on the experience of youth violence in low-income and middle-income country settings.

DESIGN: Using a standardised questionnaire, our study collected two waves of longitudinal data on sociodemographics, health practices, health outcomes and risk factors. The panel fixed-effects ordinary least squares regression models were used for the analysis.

SETTINGS: The study was conducted in 59 villages and the town of Nouna with a population of about 100 000 individuals, 1 hospital and 13 primary health centres in Burkina Faso.

PARTICIPANTS: We interviewed 1644 adolescents in 2017 and 1291 respondents in 2018 who participated in both rounds.

OUTCOME AND EXPOSURE MEASURES: We examined the experience of physical attacks in the past 12 months and bullying in the past 30 days. Our exposures were completed years of age and educational attainment.

RESULTS: A substantial minority of respondents experienced violence in both waves (24.1% bullying and 12.2% physical attack), with males experiencing more violence. Bullying was positively associated with more education (β=0.12; 95% CI 0.02 to 0.22) and non-significantly with older age. Both effects were stronger in males than females, although the gender differences were not significant. Physical attacks fell with increasing age (β=-0.18; 95% CI -0.31 to -0.05) and this association was again stronger in males than females; education and physical attacks were not substantively associated.

CONCLUSIONS: Bullying and physical attacks are common for rural adolescent Burkinabe. The age patterns found suggest that, particularly for males, there is a need to target violence prevention at younger ages and bullying prevention at slightly older ones, particularly for those remaining in school. Nevertheless, a fuller understanding of the mechanisms behind our findings is needed to design effective interventions to protect youth in low-income settings from violence.

PMID:37852761 | DOI:10.1136/bmjopen-2022-071104