Tag: pubmed

Zhonghua Zhong Liu Za Zhi. 2022 Feb 23;44(2):130-138. doi: 10.3760/cma.j.cn112152-20210208-00121.

ABSTRACT

Objective: To explore the expression of miR1290 in endometrial cancer tissues and its relationship with the pathological grade, and to find out the effect of miR1290 on biological characteristics of endometrial cancer cells and its mechanism. Methods: A total of 38 cases of endometrioid adenocarcinoma tissues, 10 cases of adjacent tissues and 23 cases of normal endometrial tissues were collected in Provincial Hospital Affiliated to Shandong University from May 2020 to October 2020. The expression of miR1290 was detected by reverse transcription polymerase chain reaction (RT-PCR). The expressions of miR1290 in endometrial cancer cells including KLE and Ishikawa were knocked down by lentiviral transfection. Cell counting kit 8 (CCK-8) test and colony formation test were used to detect cell proliferation ability, wound healing and Transwell test were used to detect cell invasion and migration ability, western blot was used to detect the expressions of epithelial-mesenchymal transition (EMT), phospholipids acylinositide 3-kinase (PI3K)/Akt and Wnt/β-catenin pathway related proteins. Results: The relative expressions of miR1290 in endometrial cancer tissues were 5.40±3.20, which was 1.55 times of normal endometrial tissues (P<0.01) and 1.75 times of adjacent tissues (P<0.01). The relative expressions of miR1290 in 17 cases of endometrial tissues at proliferative stage and 6 cases of endometrial tissues at secretory stage were 3.00±1.08 and 4.97±0.58, respectively, and the difference was statistically significant (P<0.01). In KLE cells and Ishikawa cells, the expression of miR1290 in miR1290 knockdown (Sh-miR1290) group was decreased when compared with the negative control (Sh-NC) group. The absorbance value of Sh-miR1290 group detected by the CCK-8 method and the colony formation rate detected by the colony formation experiment were both increased, the number of cells penetrating the basement membrane in the Transwell experiment and the wound healing rate in the scratch experiment were decreased (P<0.05). In KLE cells, knockdown of miR1290 reduced the expressions of EMT-related proteins including N-cadherin, Vimentin, Snail and Slug(P<0.05), and the expressions of PI3K and P-Akt/Akt (P<0.05), while there was no significant change in the expressions of Wnt and β-catenin (P>0.05). In Ishikawa cells, knockdown of miR1290 decreased the expressions of EMT-related proteins including N-cadherin, Snail and Slug, and the expressions of Wnt and β-catenin, increased the expression of E-cadherin (P<0.05), while there was no significant change in the expressions of PI3K and P-Akt/Akt (P>0.05). Conclusions: The expressions of miR1290 in endometrial cancer tissues are higher than that in the adjacent tissues and normal endometrial tissues. Knockdown of miR1290 expression can promote the proliferation of endometrial cancer cells, but inhibit cell invasion, migration and EMT ability through the PI3K/Akt and Wnt/β-catenin pathways.

PMID:35184456 | DOI:10.3760/cma.j.cn112152-20210208-00121

Zhonghua Zhong Liu Za Zhi. 2022 Feb 23;44(2):139-146. doi: 10.3760/cma.j.cn112152-20200612-00555.

ABSTRACT

Objective: To explore the effect of down-regulation of retinol binding protein 2 (RBP2) expression on the biological characteristics of ovarian cancer cells and its mechanism. Methods: Knockdown of RBP2 and cisplatin (DDP)-resistant ovarian cancer cell line SKOV3/DDP-RBP2i was established, the negative control group and blank control group were also set. Cell counting kit 8 (CCK-8) was used to detect the cell proliferation ability, flow cytometry was used to detect cell apoptosis, scratch test and Transwell invasion test were used to detect cell migration and invasion ability, real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) and western blot were used to detect the expressions of molecular markers related to epithelial-mesenchymal transition (EMT). The effect of RBP2 on the growth of ovarian cancer was verified through experiment of transplanted tumors in nude mice, and the relationships between RBP2 expression and tumor metastasis and patient prognosis were analyzed using the clinical data of ovarian cancer in TCGA database. Results: After down-regulating the expression of RBP2, the proliferation ability of SKOV3/DDP cell was significantly reduced. On the fifth day, the proliferation activities of SKOV3/DDP-RBP2i group, negative control group and blank control group were (56.67±4.16)%, (84.67±3.51) and (87.00±4.00)% respectively, with statistically significant difference (P<0.001). The apoptosis rate of SKOV3/DDP-RBP2i group was (14.19±1.50)%, higher than (8.77±0.75)% of the negative control group and (7.48±0.52)% of the blank control group (P<0.001). The number of invasive cells of SKOV3/DDP-RBP2i group was (55.20±2.39), lower than (82.60±5.18) and (80.80±7.26) of the negative control group and the blank control group, respectively (P<0.001). The scratch healing rate of SKOV3/DDP-RBP2i group was (28.47±2.72)%, lower than (50.58±4.06)% and (48.92±4.63)% of the negative control group and the blank control group, respectively (P<0.001). The mRNA and protein expressions of E-cadherin in the SKOV3/DDP-RBP2i group were higher than those in the negative control group (P=0.015, P<0.001) and the blank control group (P=0.006, P<0.001). The mRNA and protein expression of N-cadherin in SKOV3/DDP-RBP2i group were lower than those in the negative control group (P=0.012, P<0.001) and the blank control group (P=0.005, P<0.001). The mRNA and protein expressions of vimentin in SKOV3/DDP-RBP2i group were also lower than those in the negative control group (P=0.016, P=0.001) and the blank control group (P=0.011, P=0.001). Five weeks after the cells inoculated into the nude mice, the tumor volume of SKOV3/DDP-RBP2i group, negative control group and blank control group were statistically significant different. The tumor volume of SKOV3/DDP-RBP2i group was smaller than those of negative control group and blank control group (P=0.001). Bioinformatics analysis showed that the expression of RBP2 in patients with metastatic ovarian cancer was higher than that without metastasis (P=0.043), and the median overall survival of ovarian cancer patients with high RBP2 expression was 41 months, shorter than 69 months of low RBP2 expression patients (P<0.001). Conclusion: Downregulation of the expression of RBP2 in SKOV3/DDP cells can inhibit cell migration and invasion, and the mechanism may be related to the inhibition of EMT.

PMID:35184457 | DOI:10.3760/cma.j.cn112152-20200612-00555

Zhonghua Yu Fang Yi Xue Za Zhi. 2022 Feb 6;56(2):197-202. doi: 10.3760/cma.j.cn112150-20210611-00570.

ABSTRACT

Objective: To systematically evaluate the acceptance of pre-exposure prophylaxis (PrEP)among men who have sex with men (MSM) in China, so as to provide reference for the promotion of preventive drug use before human immunodeficiency virus exposure in China. Methods: By searching the databases of China national knowledge infrastructure, VIP database, Wanfan knowledge service platform, PubMed, Web of Science, Embase and The Cochrane Library with key words of “men who have sex with men” “pre-exposure prophylaxis” “PrEP” and “MSM”. The literature on the willingness of Chinese MSM population to accept PrEP was systematically collected, and the data of the literature meeting the inclusion criteria were extracted for Meta analysis. Results: A total of 12 articles were selected in this study, including 6 articles in English and 6 in Chinese. The score of bias risk assessment of eligible articles was 14-18, which was more than 70% of the total score. The total number of samples was 11 269. The overall acceptance rate of PrEP was 0.77(95%CI:0.71-0.82). In subgroup analysis, the acceptance rates of different nationalities, marriage, household registration, age, education background, income, sexual orientation, sexual behavior and awareness of PrEP were statistically significant. Conclusion: In general, the acceptance rate of PrEP in MSM population is higher, but the awareness rate is low. There are differences in the acceptance rate among different groups.

PMID:35184450 | DOI:10.3760/cma.j.cn112150-20210611-00570

Brief Bioinform. 2022 Feb 20:bbac040. doi: 10.1093/bib/bbac040. Online ahead of print.

ABSTRACT

Mass spectrometry-based proteomic technique has become indispensable in current exploration of complex and dynamic biological processes. Instrument development has largely ensured the effective production of proteomic data, which necessitates commensurate advances in statistical framework to discover the optimal proteomic signature. Current framework mainly emphasizes the generalizability of the identified signature in predicting the independent data but neglects the reproducibility among signatures identified from independently repeated trials on different sub-dataset. These problems seriously restricted the wide application of the proteomic technique in molecular biology and other related directions. Thus, it is crucial to enable the generalizable and reproducible discovery of the proteomic signature with the subsequent indication of phenotype association. However, no such tool has been developed and available yet. Herein, an online tool, POSREG, was therefore constructed to identify the optimal signature for a set of proteomic data. It works by (i) identifying the proteomic signature of good reproducibility and aggregating them to ensemble feature ranking by ensemble learning, (ii) assessing the generalizability of ensemble feature ranking to acquire the optimal signature and (iii) indicating the phenotype association of discovered signature. POSREG is unique in its capacity of discovering the proteomic signature by simultaneously optimizing its reproducibility and generalizability. It is now accessible free of charge without any registration or login requirement at https://idrblab.org/posreg/.

PMID:35183059 | DOI:10.1093/bib/bbac040

Phys Ther Sport. 2022 Feb 11;55:37-45. doi: 10.1016/j.ptsp.2022.02.003. Online ahead of print.

ABSTRACT

OBJECTIVES: To prospectively monitor biomechanics, session-rating of perceived exertion (sRPE), and wellness in a cohort of collegiate Division-1 cross-country athletes over the course of a single competitive season.

DESIGN: Prospective cohort study.

METHODS: Healthy Division-1 cross-country athletes (9 males, 13 females) were prospectively followed over a single competitive cross-country season. Wearable sensors were used to collect biomechanics twice per week, along with surveys to assess sRPE and wellness. Mixed model linear regressions were used to assess the relationship among biomechanical measures to sRPE, and to wellness z-scores.

RESULTS: Stride length, contact time, impact g, pace, weekly mileage, and running a meet in the day prior to the recorded run explained 25.4% of the variance in sRPE scores across the season (R² = 0.254, p < 0.001). Contact time and braking g helped explain 3.7% of the variance in wellness (R² = 0.037, F = 5.70, p = 0.01).

CONCLUSIONS: There were several identified associations between gait biomechanics and sRPE, yet minimal associations with wellness measures. These findings suggest there are movement adaptations associated with perceived running intensity, however biomechanical measures alone do not lend additional insight into wellness measures.

PMID:35183044 | DOI:10.1016/j.ptsp.2022.02.003

Complement Ther Clin Pract. 2022 Feb 11;47:101548. doi: 10.1016/j.ctcp.2022.101548. Online ahead of print.

ABSTRACT

BACKGROUND: Disordered eating is prevalent among US Military Veterans who have a high incidence of obesity, diabetes, and mental illness. Mindfulness is an evidenced-based intervention for some mental health disorders, is well received by Veterans, and may be useful in treating disordered eating behavior in this population. The aim of this study was to assess and describe Veterans’ experience with MB-SAVOR, a novel mindfulness-based eating program, and determine if it improved their relationship with food and the body.

METHODS: In-depth qualitative interviews were conducted among 16 Veterans completing the program. Interviews were audio recorded, transcribed, and analyzed using constant comparative method, an iterative and inductive process. Rapid assessment process was used to understand their views on program structure. Inferential statistics were conducted to assess outcomes of pre-topost-intervention weight, BMI, and HbA1C, and influences of demographics.

RESULTS: Five themes were identified related to experience: Awareness of Eating Cues, Noticing Eating Behaviors and Patterns, Greater Enjoyment of Food, Dietary Improvements, and Mind Body Connection. Four themes were identified related to program structure: Reasons for Enrollment, Prior Experiences and Comparison with MB-SAVOR, Program Information, Impression, and Barriers, and Improvement Suggestions. Clinical outcomes were decreased weight (p = 0.007, d = 0.82), BMI (p = 0.004, d = 0.9), and HbA1C (p = 0.3) post-intervention.

CONCLUSIONS: These findings contribute to our understanding of the feasibility, safety, and efficacy of MB-SAVOR on improving Veterans’ relationship with food and the body. These data help us understand Veterans’ perspectives and motivations regarding treatment engagement for several diet related problems contributing to obesity and diabetes.

PMID:35183037 | DOI:10.1016/j.ctcp.2022.101548

J Surg Res. 2022 Feb 16;274:196-206. doi: 10.1016/j.jss.2021.12.051. Online ahead of print.

ABSTRACT

BACKGROUND: The relationship between sarcopenia and postoperative outcomes in patients with gastric cancer remains controversial. This study aimed to investigate the impact of sarcopenia on short-term outcomes after surgery for gastric cancer.

METHODS: Patients who underwent surgical treatment for gastric cancer were evaluated in this prospective observational study. Muscle strength, muscle mass, and physical performance were measured before surgery. Diagnosis of sarcopenia was based on the revised European Working Group on Sarcopenia criteria. Postoperative 30-day outcomes, including complications, reoperation, readmission, and operative mortality, were recorded.

RESULTS: Sarcopenia was observed in 31 out of 146 patients (21.2%). The overall complication incidence was 31.5%. The postoperative complication rate was higher in the sarcopenic patients compared to the nonsarcopenic patients (54.8% versus 25.2%, P = 0.003). There was no statistically significant difference in terms of surgical complication rates (25.8% versus 14.8%, P = 0.239), although the sarcopenic group had a significantly higher systemic complication rate (38.7% versus 13%, P = 0.003). No statistically significant difference was observed in terms of major complications (3.2% versus 5.2%, P = 1.000). Muscle strength, muscle mass, and physical performance were not identified as independent factors when tested alone at adjusted multivariable analysis. Sarcopenia (Odds ratio: 2.73, 95% CI 1.02-7.52, P = 0.047) and severe sarcopenia (Odds ratio: 4.44, 95% CI 1.57-13.34, P = 0.006) were identified as independent prognostic factors for postoperative complications.

CONCLUSIONS: Sarcopenia was associated with postoperative complications after gastrectomy for gastric cancer. Severe sarcopenia may serve as a more robust prognostic indicator. The variation in the complication rates between sarcopenic and nonsarcopenic patients was mainly due to difference in systemic complications rather than surgical complications.

PMID:35183030 | DOI:10.1016/j.jss.2021.12.051

Mar Pollut Bull. 2022 Feb 16;176:113434. doi: 10.1016/j.marpolbul.2022.113434. Online ahead of print.

ABSTRACT

Exposure risk is assessed based on modeling suitable habitat of large pelagic fish and oil spill scenarios originating at three wells located in the western GM’s deep waters. Since the fate of the oil depends on the oceanographic conditions present during the accident, as well as the magnitude and duration of the spill, which are not known a priori, the scenarios used are a statistical representation of the area in which oil spilled from the well could be found, given all possible outcomes. The ecological vulnerability assessment identified a subset of bony fish with low-medium vulnerability and elasmobranchs with medium-high vulnerability. The oiling probability and exposure risk of both bony fish and elasmobranchs hotspots vary by well analyzed. Thus, these results provide essential information for a risk management plan for the assessed species and others with economic or conservation importance distributed in the GM and worldwide.

PMID:35183025 | DOI:10.1016/j.marpolbul.2022.113434

EBioMedicine. 2022 Feb 16;77:103883. doi: 10.1016/j.ebiom.2022.103883. Online ahead of print.

ABSTRACT

BACKGROUND: Systemic Lupus Erythematosus (SLE) is a complex and heterogeneous autoimmune disease mediated by quantities of autoantibodies in which anti-double-stranded DNA (anti-dsDNA) antibodies are important. Besides, glycosylation is one of the most commonly post-translational modifications of antibodies. The association of anti-dsDNA antibodies glycosylation and SLE disease activity is still unknown.

METHODS: We enrolled 101 consecutive treatment-naïve SLE patients with positive anti-dsDNA antibodies from the Department of Rheumatology and Immunology at Ruijin Hospital, Shanghai, between 2017 and 2019. Serum samples were used in this study. We analysed the glycosylation of anti-dsDNA IgG and total IgG subclasses according to systemic lupus erythematosus disease activity index (SLEDAI) scores. Statistical analysis and machine learning were performed to assess the correlation between glycosylation of anti-dsDNA IgG and total IgG with disease activity.

FINDINGS: Serum samples from 86 patients could be detected with anti-dsDNA IgG glycopeptide and subclass of IgG glycoform. Cluster analysis showed that glycosylation of anti-dsDNA IgG and total IgG subclasses were different in SLE patients. Fucosylation, galactosylation, and sialylation levels of anti-dsDNA IgG1 were increased with SLEDAI scores (all p<0.05). The results of machine learning showed that all the glycoforms of anti-dsDNA IgG1 had better performance with lower standardised square error (SSE) than that of total IgG1, with anti-dsDNA IgG1 fucosylation level having the lowest SSE (0.009).

INTERPRETATION: Our study indicated that glycosylation of anti-dsDNA IgG was different from that of total IgG and fucosylation of anti-dsDNA IgG1 correlated best with SLE disease activity.

FUNDING: This work is supported by the National Key Research and Development Program of China (2018YFC0910303), National Natural Science Foundation of China (81801592, 82101876), Clinical Research Plan of SHDC (SHDC2020CR4011), Ruijin Hospital Youth Incubation Project (KY2021607) and Shanghai Pujiang Young Rheumatologists Training Program (SPROG202006).

PMID:35182998 | DOI:10.1016/j.ebiom.2022.103883

Leg Med (Tokyo). 2022 Feb 12;56:102044. doi: 10.1016/j.legalmed.2022.102044. Online ahead of print.

ABSTRACT

Allele frequency distributions and statistical forensic parameters of 19 autosomal STR loci in a sample of 535 unrelated healthy Hui individuals from Yunnan province were estimated. A total of 236 alleles at these loci were identified and their corresponding allele frequencies ranged from 0.000935 to 0.527103. Penta E is the most informative in Hui population, whereas TPOX showed the lowest. All of the STR loci reached the Hardy-Weinberg equilibrium after Bonferroni correction. The combined discrimination power and probability of excluding paternity of the 19 STR loci were 0.999 999 999 999 999 999 999 984 47 16 and 0.999 999 988, respectively. Furthermore, the genetic relationship between the Yunnan Hui population and other 8 different Hui groups or 25 previously investigated groups residing in other areas of China were also estimated based on pairwise genetic distance. These results suggest that the 19 STR loci are highly polymorphic, which is suitable for forensic personal identification and paternity testing.

PMID:35182995 | DOI:10.1016/j.legalmed.2022.102044