Tag: pubmed

Wei Sheng Yan Jiu. 2021 Sep;50(5):814-820. doi: 10.19813/j.cnki.weishengyanjiu.2021.05.018.

ABSTRACT

OBJECTIVE: To investigate the effects of interleukin-17(IL-17) antibody on polarization of adipose tissue macrophages(ATM) in mice fed with high-fat diet(HFD) exposed to bisphenol A(BPA).

METHODS: Four week-old male C57 BL/6 mice were divided into control group, IgG group, IL-17 antibody group, 1000 nmol/L BPA group, 1000 nmol/L BPA+IgG group, and 1000 nmol/L BPA+IL-17 antibody group according to random number table method. Eight mice per group were fed with HFD and BPA was exposed by drinking water. The IgG group and the 1000 nmol/L BPA+IgG group were intraperitoneally injected with 100 μg IgG antibody once a week, and the IL-17 group and the 1000 nmol/L BPA+IL-17 antibody group were intraperitoneally injected with 100 μg IL-17 antibody once a week. After 16 weeks, the mice were sacrificed, and serum samples were collected for serum separation. Leptin level was measured by enzyme-linked immunosorbent assay(ELISA). The expression of IL-1β, IL-6 and monocyte chemoattractant protein-1(MCP-1)inflammatory cytokines were observed by immunohistochemistry(IHC) of adipose tissue of epididymis. The activity of inducible nitric oxide synthase(iNOS)and arginase-1(Arg-1)was measured by ELISA, and the proportion of M1 and M2 ATMs was measured by flow cytometry(FCM). The expression of CD11 c and CD206 mRNA was measured by qRT-PCR.

RESULTS: Compared with the control group, serum leptin, the expressions of inflammatory cytokines IL-6, IL-1β and MCP-1 in adipose tissue were increased in 1000 nmol/L BPA group, the proportion of M1 type ATM was increased(22.000%±0.500% vs. 31.467%±0.379%), iNOS activity was increased, CD11 c mRNA expression was increased, Arg-1 activity was decreased, CD206 mRNA expression was decreased, the differences were statistically significant(P<0.05), but the proportion of M2 type ATM was decreased insignificantly(P>0.05). There was no significant change in IgG group. Compared with 1000 nmol/L BPA group, IgG+1000 nmol/L BPA group had no significant change. In 1000 nmol/L BPA+IL-17 antibody group, serum leptin was decreased, the expressions of inflammatory factors IL-6, IL-1β and MCP-1 in adipose tissue were down-regulated, and the proportion of M1 type ATM was decreased(31.467%±0.379% vs. 22.933%±0.153%), iNOS activity was decreased, CD11 c mRNA expression was decreased, and the proportion of M2 type ATM was increased(4.847%±0.655% vs. 7.840%±0.555%), Arg-1 activity was enhanced, and CD206 mRNA expression was up-regulated, the differences were statistically significant(P<0.05).

CONCLUSION: IL-17 antibody may reduce the secretion of ATM inflammatory factors by inhibiting the polarization of ATM to M1 type, thus improving the inflammation of adipose tissue in BPA-infected HFD-fed mice.

PMID:34749877 | DOI:10.19813/j.cnki.weishengyanjiu.2021.05.018

Wei Sheng Yan Jiu. 2021 Sep;50(5):769-774. doi: 10.19813/j.cnki.weishengyanjiu.2021.05.011.

ABSTRACT

OBJECTIVE: To study the distribution characteristics of atmospheric pollutants and their correlation with meteorological factors.

METHODS: The PM_(2.5), PM_(10), NO_2, SO_2, O_(3-1 h), O_(3-8 h) and CO daily average concentration data from 2014 to 2020 were obtained from Lanzhou Environmental Protection Bureau. The interannual changes of pollutants, monthly changes, seasonal changes and trend of spatial distribution were analysed. Spearman correlation analysis was performed on the relationship among pollutants.

RESULTS: The main pollutants exceeding the standard in Lanzhou from 2014 to 2020 were PM_(10), PM_(2.5) and NO_2, average annual concentration of PM_(10), PM_(2.5), NO_2 and CO were decreasing year by year, and O_3 was increasing year by year. The monthly average concentration of PM_(10) was the highest in December, January, March and November were the second highest, and it was higher in February, April and May. The monthly change trends of PM_(2.5), NO_2, SO_2 and CO concentrations were the same, and the monthly change trends of the 1 hour average and daily maximum 8-hour average concentrations of O_3 were the same. The seasonal variation of atmospheric pollutant concentration was obvious, the concentrations of PM_(10), PM_(2.5), SO_2, NO_(2 )and CO were the highest in winter and the lowest in summer. O_3 concentration was the highest in summer and lowest in winter. Average annual concentration of 6 pollutants in different regions had statistically significant differences(H=750.40, 1112.99, 1410.05, 352.04, 360.17, 619.20, 729.52; P<0.001). Among them, the average annual concentration of PM_(10), PM_(2.5), SO_(2 )and O_(3-1 h) in Xigu District were the highest. PM_(10), PM_(2.5), NO_2, SO_2, CO average annual concentration were negatively correlated with temperature and wind speed(r_s=-0.423, -0.561, -0.395, -0.660, -0.569, -0.043, -0.094, -0.130, -0.172, -0.135), the concentration of PM_(10), PM_(2.5), O_3, SO_2 concentration were negatively correlated with humidity(r_s=-0.238, -0.121, -0.110, -0.094), only O_3 was positively correlated with temperature(r_s=0.486).

CONCLUSION: The primary pollutants in Lanzhou from 2014 to 2020 were PM_(10), PM_(2.5) and NO_2.O_3 had an obvious upward trend year by year. The 6 pollutants had obvious seasonal changes and regional distribution characteristics. Some pollutants had the same homology, and meteorological factors affected each pollution. The concentration of the substance had an important influence. Relevant air pollution control measures should be formulated based on the main excessive pollutants, the monthly change trend of air pollution and the seasonal pollution characteristics, the same emission sources and geographical distribution characteristics should be considered, and the meteorological factors should be combined.

PMID:34749870 | DOI:10.19813/j.cnki.weishengyanjiu.2021.05.011

Wei Sheng Yan Jiu. 2021 Sep;50(5):781-787. doi: 10.19813/j.cnki.weishengyanjiu.2021.05.013.

ABSTRACT

OBJECTIVE: To investigate the protective effect of oleanolic acid(OA) on HgCl_2 induced liver injury.

METHODS: L02 cells were divided into four groups according to different treatment, control group(Con), oleanolic acid group(OA, 10 μmol/L), HgCl_2 group(HgCl_2, 40 μmol/L) and oleanolic acid + HgCl_2 group(OA + HgCl_2). Cells of control group were given serum-free medium, cells of OA group were pretreated with OA solution for 8 hours, cells of HgCl_2 group were exposed to HgCl_2 solution for 6 hours, cells of OA + HgCl_2 group were pretreated with OA solution for 8 hours, and then exposed to HgCl_2 solution for 6 hours. MTT assay was used to detect cell viability. Laser confocal scanning was used to detect JC-1 probe fluorescence intensity to determine mitochondrial membrane potential. DCFH-DA fluorescence probe combined with flow cytometry was used to detect reactive oxygen species(ROS) level. Annexin V/PI double staining method combined with flow cytometry was used to determine cell apoptosis rate. Catalase(CAT), total superoxide dismutase(T-SOD), glutathione(GSH), malondialdehyde(MDA), Caspase 3 and Caspase 9 kits combined with enzyme labeled instrument were used to determine their activity or content respectively.

RESULTS: Compared with the control group, 40 μmol/L HgCl_2 could significantly reduce cell viability, the level was 0.52±0.03(P<0.05), OA pretreatment could significantly inhibit the decrease of cell viability induced by HgCl_2, the level was 0.86±0.05(P<0.05). The result of mitochondrial membrane potential detection showed that cell exposed to 40 μmol/L HgCl_2 significantly reduced the intensity of red fluorescence, and the ratio of red to green fluorescence was 0.23±0.02(P<0.05). OA pretreatment significantly increased red fluorescence, and the ratio of red fluorescence to green fluorescence was 1.32±0.08, which was significantly higher than that of HgCl_2(P<0.05). After exposure to 40 μmol/L HgCl_2, the relative fluorescence intensity of ROS was 1.21±0.07, the apoptosis rate was about 8%, the activity levels of Casepase 3 and Casepase 9 were 3.11±0.20 and 2.94±0.17, respectively, which were all significantly higher than those in the control group(P<0.05). OA pretreatment could significantly alleviate the changes of the above indexes, and the difference was statistically significant compared with HgCl_2 group(P<0.05). The level of T-SOD in HgCl_2 group was(7.68±0.39)U/mL, which was significantly lower than that in control group(P<0.05). Compared to the control group, the level of MDA was significantly increased to(4.99±0.26)nmol/mg(P<0.05). OA pretreatment significantly increased level of T-SOD and decreased the level of MDA, the levels were(13.97±0.71)U/mL and(3.01±0.17)nmol/mg, respectively(P<0.05).

CONCLUSION: A certain concentration of HgCl_2 can induce hepatocyte damage. OA pretreatment may reduce cell damage by improving oxidative stress.

PMID:34749872 | DOI:10.19813/j.cnki.weishengyanjiu.2021.05.013

Wei Sheng Yan Jiu. 2021 Sep;50(5):763-774. doi: 10.19813/j.cnki.weishengyanjiu.2021.05.010.

ABSTRACT

OBJECTIVE: The study aimed at association of family life, resilience and bullying on the use of tobacco in preadolescents.

METHODS: A total of 4792 students from 5 junior schools in Baise City were recruited with cluster-sampling method, filled with questionnaire of family life, resilience, parents' Control, bullying, initiation of tobacco and smoking from Feb. to Nov. 2018.The sample comprised of 52.63% male students and 46.66% female students. The average age was(11.8±0.5). There were 56.78% of students lived in city and 43.22% of students lived in county town; The nationality of the sample was as follows: Zhuang nationality 90.00%, Han nationality 7.62%, other minorities(Yao nationality, Miao nationality, Yi nationality, et al)2.05%. The Logistic regression was used to explore the effect.

RESULTS: There were 9.75% and 6.97% of the sample reported initiation of tobacco and smoking respectively. The initiation of tobacco and smoking of boys were higher than that of girls(initiation of tobacco: χ~2=57.230, P<0.001; smoking: χ~2=56.013, P<0.001). The multivariate Logistic analysis showed gender was statistically significant factor of initiation of tobacco(OR=0.468, 95%CI 0.377-0.582) and smoking(OR=0.422, 95% CI 0.324-0.551), and age was statistically significant factor of initiation of tobacco(OR=1.609, 95% CI 1.446-1.791) and smoking(OR=2.026, 95%CI 1.776-2.310). Bullying was statistically significant factors of smoking(OR=1.106, 95% CI 1.073-1.140). Three protective factors were associated with a lower likelihood of initiation of tobacco(individual power: OR=0.964, 95% CI 0.951-0.976; family cohesion, OR=0.946, 95% CI 0.892-0.984; family rules, OR=0.949, 95%CI 0.930-0.965) and smoking(individual power: OR=0.962, 95% CI 0.947-0.977; family cohesion, OR=0.937, 95%CI 0.885-0.992; family rules, OR=0.952, 95%CI 0.932-0.973)in the final subscale model.

CONCLUSION: Bullying increased the risk of smoking, while Individual power, family cohesion and family rules were associated with a lower likelihood of initiation of tobacco and smoking in preadolescents.

PMID:34749869 | DOI:10.19813/j.cnki.weishengyanjiu.2021.05.010

Wei Sheng Yan Jiu. 2021 Sep;50(5):735-755. doi: 10.19813/j.cnki.weishengyanjiu.2021.05.006.

ABSTRACT

OBJECTIVE: To investigate the level of yogurt intake in the Chinese population and its relationship between the level of yogurt intake and metabolic syndrome.

METHODS: Samples were taken from populations in 8 cities in China. Dietary surveys, physical examinations, and blood sample were collected. The level of yogurt intake of the population were calculated and evaluated. The relationship between yogurt intake and metabolic syndrome and its components was analyzed by multiple logistic regression. The yogurt intake was investigated using a diet frequency questionnaire to record the frequency and intake of yogurt in the past month.

RESULTS: A total of 1508 respondents were included in this study, including 538 males and 970 females; the average age was 51.74 years; the distribution ratio in the North and South regions was 5∶4.The rate of Chinese population in 8 cities which eat yogurt was 50.1%. The intake of yogurt was 3.7 g/d. Yogurt accounts for 27.22% of dairy products. There were differences in the distribution of different yogurt intake groups in different genders, age groups, Body Mass Index(BMI)groups, regions, education levels, monthly income, and smoking. The differences in calcium, fruit, and total dairy product intake among different yogurt intake groups were statistically significant difference. Sample analysis found that yogurt intake was negatively correlated with metabolic syndrome and its components. We adjusted gender, age group, body mass index group, region, education grade, monthly income, smoking, total energy, protein, fat, Ca, fruit, total dairy products for multi-factor analysis and found that this negative correlation was weakened. But this negative correlation remained on abnormal blood glucose[OR=0.61(95%CI 0.42-0.89)].

CONCLUSION: The yoghurt intake of Chinese residents is low. The intake of yogurt has a negative correlation with abnormal blood glucose.

PMID:34749865 | DOI:10.19813/j.cnki.weishengyanjiu.2021.05.006

Chirurgia (Bucur). 2021 Oct;116(5):620-626. doi: 10.21614/chirurgia.116.5.620.

ABSTRACT

Background: The increased incidence of both colorectal cancer and diabetes mellitus, as well as the fact that they are important causes of high morbidity and especially mortality, place the two pathologies on the list of priorities of the health system. There are studies which have observed that diabetes mellitus is directly involved in carcinogenesis and is an independent risk factor for colorectal cancer. The diabetic patient requires a complex medical-surgical approach, so setting the risk criteria for them can be the starting point for improving the survival rate. Materials and methods: The association between colorectal cancer and diabetes mellitus as well as screening criteria were analyzed in a descriptive prospective study (442 patients) conducted in the Dr. I. Cantacuzino Clinical Hospital during 2017-2018. In the study, patients were distributed in two clusters, one with diabetes mellitus (N = 194) and one without diabetes mellitus (N = 248) in which basic clinical and laboratory evaluations were performed followed by screening colonoscopies. Results: Statistically significant (p 0.005) correlations were highlighted between 7 of the variables tested and the positive results on colonoscopy, which were subsequently combined to achieve a risk score. Conclusions: Diabetes mellitus is an independent risk factor and a negative prognostic factor for colorectal cancer. The risk score described as a result of this study is a feasible, simple solution for early detection of precursor lesions or neoplasms with the ultimate goal of improving the prognosis and survival of these patient.

PMID:34749858 | DOI:10.21614/chirurgia.116.5.620

Wei Sheng Yan Jiu. 2021 Sep;50(5):728-734. doi: 10.19813/j.cnki.weishengyanjiu.2021.05.005.

ABSTRACT

OBJECTIVE: To explore whether the association of community urbanization levels with overweight incidence changes over time and varies by region.

METHODS: The data is from the China Health and Nutrition Survey(1991-2015). The survey applied a multistage, random cluster design to draw samples. A total of 8684 adults aged 20-64 years were confined in nine provinces(autonomous regions). Based on an urbanicity score, communities were divided into two groups: low-and high-urbanization-level groups. The nine provinces(autonomous regions) were divided into four regions, including the Coast, Central, Northeast and West. Multilevel Logistic regression models was applied to examine whether the association between urbanization levels and overweight incidence changed over time and varied by region.

RESULTS: 38.08% of respondents became overweight during the follow-up surveys. The interactions between the high urbanization level with the Coast(OR=0.45, 95%CI 0.34-0.60), the Central(OR=0.67, 95%CI 0.52-0.86), and the Northeast(OR=0.70, 95%CI 0.52-0.94) were statistically significant. The interaction between the high urbanization level and period was also statistically significant(OR=0.97, 95%CI 0.96-0.99). In 1991, the Coast(OR=0.99, 95%CI 0.77-1.26), the high urbanization level was positively associated with overweight incidence in the Central(OR=1.45, 95%CI 1.15-1.83), Northeast(OR=1.53, 95%CI 1.16-2.02) and West(OR=2.18, 95%CI 1.68-2.83), with statistical significance. In 2015, the high urbanization level was significantly negatively associated with overweight incidence in the Coast(OR=0.53, 95%CI 0.41-0.68) and Central(OR=0.77, 95%CI 0.62-0.97), while the association in the Northeast(OR=0.82, 95%CI 0.63-1.05) and West(OR=1.17, 95%CI 0.91-1.49) was statistically insignificant.

CONCLUSION: There were spatiotemporal variations in the association between urbanization levels and overweight incidence among adults in the nine provinces(autonomous regions).

PMID:34749864 | DOI:10.19813/j.cnki.weishengyanjiu.2021.05.005

Chirurgia (Bucur). 2021 Oct;116(5):599-608. doi: 10.21614/chirurgia.116.5.599.

ABSTRACT

Introduction: Abdominal wall surgery for parietal defects is done by implanting a type of mesh in the surrounding tissue above or beneath the fascia layer of the abdominal wall. The most common type of mesh used is polypropylene which sometimes takes a lot of time to be covered by the fibrous tissue. In an attempt to accelerate the cellular binding on the mesh and so to increase the recovery rate, we developed a protocol with plasma derived products to accelerate the mesh integration. Platelet rich fibrin (PRF) and platelet rich plasma (PRP) were evaluated in promoting the collagen synthesis and cell proliferation on the mesh surface. Material and Methods: We evaluated 32 patients with different types of abdominal wall defects which required polypropylene mesh implants in open surgery with the mesh implanted above the aponeurosis layer. We divided the patients into 3 groups: standard procedure, mesh augmented with PRF only, mesh augmented with PRP only. Results: Even though the number of patients involved in the study has a very small impact for a statistical analysis, the pattern observed in our prospective study reveals from the beginning that augmenting the standard procedure with plasma derived products improve the outcome (mesh integration) up to 65% faster integration. Conclusion: The technique that we used to augment the standard implant is cost-effective and simple to use in the surgical theatre.

PMID:34749856 | DOI:10.21614/chirurgia.116.5.599

Chirurgia (Bucur). 2021 Oct;116(5):554-567. doi: 10.21614/chirurgia.116.5.554.

ABSTRACT

Background: We present a comparative analysis of survival, complications and major risk factors in patients who underwent surgery for pancreatic head carcinoma. Methods: This is a single-centre retrospective study aimed to evaluate clinical, surgical and pathoanatomical features of 467 patients who underwent radical surgery for pancreatic head carcinoma between September 2004 and October 2019. The series includes 88 patients (18.8%) with venous resections for borderline resectable pancreatic adenocarcinoma. Results: The estimated median survival rates were statistically significant with 19.3 months in pancreatoduodenal venous resections (VR) and 26.9 months in pancreatoduodenal resections (PDR), respectively (p=0.047). On the other hand, one, three, and five-year survival rates of 46.6%, 17.6% and 8.3% in VR, and 53.6%, 20.8%, 14.9% in PDR were not statistically significant (p=0.13, 0.5 and 0.11 respectively). Survival rates comparison in PDR, VR, and palliative procedures (PP) between the three groups showed statistical significance (p 0.05). The clinically relevant postoperative complications in venous resections (13.6%) vs. 14.8% in PDR were not statistically significant (p=0.77). Postoperative bleeding and reoperation (p 0.05) are independent prognostic factors for worse outcomes. There was no statistically significant relationship between survival and presence of vascular invasion (p=0.581). Conclusions: When performed by experienced surgeons at specialized high-volume centres, pancreatoduodenal resections combined with venous resection and reconstruction are reliable and safe surgical procedures.

PMID:34749851 | DOI:10.21614/chirurgia.116.5.554

Chirurgia (Bucur). 2021 Oct;116(5):591-598. doi: 10.21614/chirurgia.116.5.591.

ABSTRACT

Introduction: A patent digestive anastomosis is not only the result of the surgery team experience, but also dependent on the patient̢??s factors. Accepting the possible dramatic effects of an anastomotic leak, identification of risk factors remains a priority in case management. Material and methods: Multifactorial assessment scores permit risk quantification, increase grade of suspicion and early management implementation. The correlation between diverse potential risk factors and anastomotic leak (AL) was studied. The identified risk factors were included in a predictive score system. FISTULA SCORE represents a feasible instrument based on 12 clinical, paraclinical and therapeutic variables, with good statistical significance (Se = 79.5%, Sp = 90.2%). Results: Anastomotic leaks (AL) were observed in 39 cases (7.68%) out of 508 patients analysed, appearing in days 2 Р10 after surgery, with a mean value of 6 days. FISTULA SCORE was based on attributed risks found in our study group for each factor and has the purpose to identify patients at risk for AL and, in some cases, to change the therapeutic or surgical strategy. In AL patients group, the mean score was 5.06 1.95 points, and in AL-free patients group Р1.57 1.61 points. Conclusions: The risk for AL must be appreciated and quantified with a multivariable scoring system. FISTULA SCORE can identify, with a good statistical significance, patient at risk for AL, changing the management of case, reducing length of stay, costs, morbidities, mortality and psychological effects on patient and medical stuff.

PMID:34749855 | DOI:10.21614/chirurgia.116.5.591