Tag: pubmed

J Clin Pediatr Dent. 2021 Nov 1;45(5):298-305. doi: 10.17796/1053-4625-45.5.2.

ABSTRACT

OBJECTIVE: The aim of this study is to evaluate the relationship between packaged product label and medication package insert reading habits of parents and their children’s oral/dental health.

STUDY DESIGN: A questionnaire including demographic characteristics and label/insert reading habits was filled by parents of 301 children who referred to the Pediatric Dentistry Department. The children were examined intraorally and dmft/DMFT and ICDAS II scores were recorded. The data were evaluated statistically.

RESULTS: Label and medication package insert reading were found in 71.4% and 88.4% of the parents, respectively. Label reading increased as the age of the child and the number of children in the family increased. Medical package insert reading increased as the mother’s education and SLS/paraben knowledge increased. Decrease of 1 point in ICDAS II resulted in the 1.410 times increase in the rate of medical package insert reading of the parents.

CONCLUSION: It is concluded that improving the label and medical insert reading rate of the parents would be effective for providing better oral and dental health for their children.

PMID:34740268 | DOI:10.17796/1053-4625-45.5.2

J Clin Pediatr Dent. 2021 Nov 1;45(5):306-311. doi: 10.17796/1053-4625-45.5.3.

ABSTRACT

INTRODUCTION: Bioceramic materials, gray and white mineral trioxide aggregate (GMTA, WMTA), have been shown to have high rates of success in various endodontic applications. A major drawback is their tendency to discolor teeth compared to Biodentine (BD), that has been claimed not to discolor teeth. The aim of this study was to compare tooth discoloration after applying different pulpotomy base materials (BD, GMTA and WMTA).

STUDY DESIGN: Forty human incisors teeth were used in this study. Coronal access was achieved by a Tungsten Carbide drill, and the pulp chambers were accessed and chemo-mechanically debrided. Each material was placed in the pulp chamber, up to the cervical sectioning level. All specimens were incubated at 37°C and 100% humidity for three months and have been evaluated before the study and weekly. Color was assessed according to the CIE L*a*b* color space system.

RESULTS: ΔE of all experimental groups (GMTA, WMTA and BD) were significantly different from the control group at all time points (P<0.05). Color changes in the GMTA and WMTA groups, had no statistically significant differences, but showed higher discoloration compared to BD group in the cervical part of the crown, since week 1 (P<0.05). WMTA group showed significant discoloration in the cervical part as of week 1 (P<0.05), and gradually increased over time (Figure 2). BD group showed no significantly discoloration over time. GMTA group showed the significant discoloration at week 1 and week 14 (P<0.05).

CONCLUSIONS: both GMTA and WMTA pulpotomy materials may discolor tooth structure over time in an extracted permanent anterior tooth model. When choosing bioceramic pulpotomy material, BD may be preferable in esthetic area.

PMID:34740264 | DOI:10.17796/1053-4625-45.5.3

J Clin Pediatr Dent. 2021 Nov 1;45(5):317-322. doi: 10.17796/1053-4625-45.5.5.

ABSTRACT

PURPOSE: To evaluate the effects of different treatment time of 5.25% Sodium hypochlorite (NaOCl) on the microshear bond strength (μSBS), attenuated total reflection Fourier transform infrared (ATR-FTIR) and etching pattern in mild and moderate fluorotic enamel.

STUDY DESIGN: Forty-eight fluorotic molars were divided into two groups: mild and moderate fluorotic enamel which were classified by a Thylstrup and Fejerskov index (TFI). Based on the application time (0s, 60s, 120s, 180s) of 5.25% NaOCl, each group was sectioned into four parts. Then the etched enamel was bonded with resin and tested to acquire μSBS. The statistical method was two-way ANOVA and Least Significant Difference (LSD) test at α = 0.05. Besides, fracture modes were observed under a stereo microscope. SEM was used to evaluated the enamel-etching pattern and organic content on the fluorotic enamel surface were investigated by ATR-FTIR.

RESULTS: Duration of 5.25% NaOCl at 60s or 120s significantly increased the μSBS of fluorotic enamel compared to 0s (p<0.05). Fracture modes indicated that dominating failures were set in the bonding interface but whose proportion decreased when 5.25% NaOCl was applied. The enamel-etching pattern in 180s was deepest under SEM. Spectra of enamel samples manifested an obvious and gradual removal of its organic phase after duration of NaOCl increased.

CONCLUSION: The maximal μSBS is acquired by using 5.25% NaOCl at 60s for mild fluorotic enamel but 120s for the moderate. The prolonged application time of 5.25% NaOCl prior to phosphoric acid etching improves enamel-etching pattern. Treatment of 5.25% NaOCl decreases proteins on the fluorotic enamel surface.

PMID:34740266 | DOI:10.17796/1053-4625-45.5.5

J Clin Pediatr Dent. 2021 Nov 1;45(5):359-370. doi: 10.17796/1053-4625-45.5.12.

ABSTRACT

BACKGROUND: The aim of this study is to investigate the relative contributions of genetic and environmental factors to variations in dental dimensions in a sample of Turkish twins, and to estimate heritability using dental casts.

STUDY DESIGN: The study samples were selected from the twin children between 3-15 years old who referred for their first dental examination. Fifty nine monozygotic and one hundred and forty three dizygotic twin pairs were examined in the study. The alginate impression material used to create the plaster model of maxilla and mandible. Anterior arch width, posterior arch width, arch length and arch circumference were measured on models prepared from measurements taken for both maxilla and mandible with digital caliper. The similarities and differences of the measurements were compared between pairs of twins and zygocytes. Morever, the effects of bad oral habits, bruxism, a result of psychosocial factors on measurements were examined. Statistical analysis was performed using Paired T Test, Wilcoxon Test and Mann Whitney U test.

RESULTS: A total of 404 dental models of 118 (29.2%) monozygotic and 286 (70.8%) dizygotic twins were evaluated. There was no statistical difference between sibling pairs in both monozygotic and dizygotic twins. The measurement similarity between twin siblings differed according to zygosity in all measurements (p<0.05). It has been observed that the finger sucking and mouth breathing affect the dental arch measurements (p<0.05).

CONCLUSION: These results indicate that the differences in dental arch dimensions between monozygotic twin pairs are less than the difference between dizygotic twin pairs.

PMID:34740261 | DOI:10.17796/1053-4625-45.5.12

J Clin Pediatr Dent. 2021 Nov 1;45(5):330-336. doi: 10.17796/1053-4625-45.5.7.

ABSTRACT

PURPOSE: This study aims to determine the relation of salivary LL37 level and mutans streptococci levels in early childhood caries (ECC).

STUDY DESIGN: A case-control study was performed in children ≤71 months old. Unstimulated whole saliva was collected and the level of salivary LL37 was measured using an enzyme-linked immunosorbent assay kit. The mutans streptococci oral bacteria were isolated from saliva and identified using a modified SB-20 culture medium (SB-20M). Data were analyzed using descriptive statistics, bivariate, and Spearman’s rank correlation analysis.

RESULTS: The was a variability of salivary LL37 level among the children and the level was significantly associated with age and races. The median (IQR) value of salivary LL37 in caries-free (CF) children was significantly higher 393.50 (580.55) ng/mL compared to 172.50 (234.65) ng/mL in the ECC group. The ECC children exhibited a significantly higher count of S. mutans and S. sobrinus compared to the CF children. An inverse weak correlation between salivary LL37 and dmft was also observed.

CONCLUSIONS: The low salivary LL37 level and higher S. mutans and S. sobrinus count in ECC supported the protective role of salivary LL37 against dental caries. Further studies are required to explore the definite relation between salivary LL37 levels and dental caries.

PMID:34740262 | DOI:10.17796/1053-4625-45.5.7

Genetics. 2021 Nov 5;219(3):iyab115. doi: 10.1093/genetics/iyab115.

ABSTRACT

The Beavis effect in quantitative trait locus (QTL) mapping describes a phenomenon that the estimated effect size of a statistically significant QTL (measured by the QTL variance) is greater than the true effect size of the QTL if the sample size is not sufficiently large. This is a typical example of the Winners’ curse applied to molecular quantitative genetics. Theoretical evaluation and correction for the Winners’ curse have been studied for interval mapping. However, similar technologies have not been available for current models of QTL mapping and genome-wide association studies where a polygene is often included in the linear mixed models to control the genetic background effect. In this study, we developed the theory of the Beavis effect in a linear mixed model using a truncated noncentral Chi-square distribution. We equated the observed Wald test statistic of a significant QTL to the expectation of a truncated noncentral Chi-square distribution to obtain a bias-corrected estimate of the QTL variance. The results are validated from replicated Monte Carlo simulation experiments. We applied the new method to the grain width (GW) trait of a rice population consisting of 524 homozygous varieties with over 300 k single nucleotide polymorphism markers. Two loci were identified and the estimated QTL heritability were corrected for the Beavis effect. Bias correction for the larger QTL on chromosome 5 (GW5) with an estimated heritability of 12% did not change the QTL heritability due to the extremely large test score and estimated QTL effect. The smaller QTL on chromosome 9 (GW9) had an estimated QTL heritability of 9% reduced to 6% after the bias-correction.

PMID:34740243 | DOI:10.1093/genetics/iyab115

J Clin Pediatr Dent. 2021 Nov 1;45(5):352-358. doi: 10.17796/1053-4625-45.5.11.

ABSTRACT

AIM: To evaluate the effects produced by functional orthodontic appliances at dental and skeletal level in relation to the level of skeletal maturation in class II patients.

STUDY DESIGN: Longitudinal and observational study. Patients selected for the study had been wearing Sander Bite Jumping Appliance (SBJA) for at least 12 months; they were first diagnosed (T1) with skeletal class II according to Ricketts’ cephalometric analysis, and had had lateral cephalograms taken before and after orthopaedic treatment (T2). Variables studied at T1 and T2 were: facial convexity, inclination of the upper and lower incisors, and facial depth. Results were compared between T1 and T2 for each variable and in relation to cervical maturation stage (CVS) according to the Lamparski analysis. Statistical analysis was performed using Shapiro-Wilk, t-student, Analysis of Variance (ANOVA) and multiple comparison tests, taking as statistically significant a p-value <0.05.

RESULTS: A final sample of 235 patients was obtained. Statistically significant differences were found in the inclination of the mandibular incisors between T1 and T2 and among the different cervical stages when the functional appliances were placed in CVS1 (p = 0.000), CVS2 (p = 0.04) or CVS5 (p = 0.048). For the remaining variables, significant differences were also found between T1 and T2, but these differences were similar in all cervical stages.

CONCLUSIONS: A significant proclination of the mandibular incisors was found when the functional appliance was placed during CVS1, CVS2, or CVS5. Time of placement of the functional appliances was not statistically significant for the remaining variables studied.

PMID:34740259 | DOI:10.17796/1053-4625-45.5.11

Genetics. 2021 Nov 5;219(3):iyab140. doi: 10.1093/genetics/iyab140.

ABSTRACT

Transcriptional regulatory networks (TRNs) are enriched for certain “motifs.” Motif usage is commonly interpreted in adaptationist terms, i.e., that the optimal motif evolves. But certain motifs can also evolve more easily than others. Here, we computationally evolved TRNs to produce a pulse of an effector protein. Two well-known motifs, type 1 incoherent feed-forward loops (I1FFLs) and negative feedback loops (NFBLs), evolved as the primary solutions. The relative rates at which these two motifs evolve depend on selection conditions, but under all conditions, either motif achieves similar performance. I1FFLs generally evolve more often than NFBLs. Selection for a tall pulse favors NFBLs, while selection for a fast response favors I1FFLs. I1FFLs are more evolutionarily accessible early on, before the effector protein evolves high expression; when NFBLs subsequently evolve, they tend to do so from a conjugated I1FFL-NFBL genotype. In the empirical S. cerevisiae TRN, output genes of NFBLs had higher expression levels than those of I1FFLs. These results suggest that evolutionary accessibility, and not relative functionality, shapes which motifs evolve in TRNs, and does so as a function of the expression levels of particular genes.

PMID:34740240 | DOI:10.1093/genetics/iyab140

Clin Spine Surg. 2021 Nov 4. doi: 10.1097/BSD.0000000000001271. Online ahead of print.

ABSTRACT

STUDY DESIGN: Retrospective cohort study.

OBJECTIVE: The aim were to (1) evaluate differences in postoperative outcomes and cost associated with outpatient anterior lumbar interbody fusion (ALIF) compared with inpatient ALIF, and to (2) identify independent factors contributing to complications after outpatient ALIF.

SUMMARY OF BACKGROUND: While lumbar fusion is traditionally performed inpatient, outpatient spinal surgery is becoming more commonplace as surgical techniques improve.

METHODS: The study population included all patients below 85 years of age who underwent elective ALIF (CPT-22558). Patients were then divided into those who underwent single-level fusion and multilevel fusion using the corresponding additional level fusion codes (CPT-22585). These resulting populations were then split into outpatient and inpatient cohorts by using a service location modifier. To account for selection bias, propensity score matching was performed; the inpatient cohorts were matched with respect to the outpatient cohorts based on age, sex, and Charlson Comorbidity Index. Statistical significance was set at P<0.05 and the Bonferroni correction was used for each multiple comparison (P<0.004).

RESULTS: Patients undergoing outpatient procedure had decreased rates of medical complications following both single-level and multilevel ALIF. In addition, age above 60, female sex, Charlson Comorbidity Index>3, chronic obstructive pulmonary disease, diabetes mellitus, coronary artery disease, hypertension, and tobacco use were all identified as independent risk factors for increased complications. Finally, the cost of outpatient ALIF was $12,013 while the cost of inpatient ALIF was $27,271 (P<0.001).

CONCLUSION: The findings add to the growing body of literature advocating for the utilization of ALIF in the outpatient setting for a properly selected group of patients.

LEVEL OF EVIDENCE: Level IV.

PMID:34740230 | DOI:10.1097/BSD.0000000000001271

Genetics. 2021 Nov 5;219(3):iyab124. doi: 10.1093/genetics/iyab124.

ABSTRACT

Over the last decade, multiparental populations have become a mainstay of genetics research in diploid species. Our goal was to extend this paradigm to autotetraploids by developing software for quantitative trait locus (QTL) mapping in connected F1 populations derived from a set of shared parents. For QTL discovery, phenotypes are regressed on the dosage of parental haplotypes to estimate additive effects. Statistical properties of the model were explored by simulating half-diallel diploid and tetraploid populations with different population sizes and numbers of parents. Across scenarios, the number of progeny per parental haplotype (pph) largely determined the statistical power for QTL detection and accuracy of the estimated haplotype effects. Multiallelic QTL with heritability 0.2 were detected with 90% probability at 25 pph and genome-wide significance level 0.05, and the additive haplotype effects were estimated with over 90% accuracy. Following QTL discovery, the software enables a comparison of models with multiple QTL and nonadditive effects. To illustrate, we analyzed potato tuber shape in a half-diallel population with three tetraploid parents. A well-known QTL on chromosome 10 was detected, for which the inclusion of digenic dominance lowered the Deviance Information Criterion (DIC) by 17 points compared to the additive model. The final model also contained a minor QTL on chromosome 1, but higher-order dominance and epistatic effects were excluded based on the DIC. In terms of practical impacts, the software is already being used to select offspring based on the effect and dosage of particular haplotypes in breeding programs.

PMID:34740237 | DOI:10.1093/genetics/iyab124