Tag: pubmed

Braz Oral Res. 2021 Dec 17;35:e140. doi: 10.1590/1807-3107bor-2021.vol35.0140. eCollection 2021.

ABSTRACT

The current study evaluated the effects of taxifolin treatments on the viability of osteoblast-like cells, and on the expression of early mineralization markers, as part of the ongoing search for new endodontic materials able to induce periapical healing without causing cytotoxicity. Saos-2 osteoblast-like cells were exposed to different concentrations of taxifolin (5 and 10 µM), applied as pretreatments either for 24h and 72h, or continuously throughout the experimental protocol. Cell viability using the methylthiazole tetrazolium (MTT) assay, alkaline phosphatase activity using thymolphthalein monophosphate assays, deposition of mineralized nodules using alizarin red staining, and expression of ALP and COL-1 by qPCR were determined after 6 and 13 days of treatment. The data were analyzed statistically (p<0.05). Taxifolin was not cytotoxic in the concentrations tested. Pretreatments with taxifolin for 24h and 72h at 10 µM stimulated ALP activity, and increased mineralized nodule deposition by Saos-2 cells. Continuous treatment with taxifolin was not effective in stimulating ALP activity and mineralization. ALP and COL-1 gene expression increased with taxifolin pretreatments, since the highest mRNA levels were observed after 72h of pretreatment with taxifolin at 10 µM on day 13. In conclusion, taxifolin was cytocompatible, and induced mineralization markers when applied for short periods in osteoblast-like cell culture.

PMID:34932669 | DOI:10.1590/1807-3107bor-2021.vol35.0140

Braz J Biol. 2021 Dec 15;84:e253514. doi: 10.1590/1519-6984.253514. eCollection 2021.

ABSTRACT

The objective of the current study was to investigate the synergistic impact of α-Tocopherol and α-Linolenic acid (100 µM) on IVM and IVC of Nili Ravi buffalo oocytes. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. Buffalo cumulus oocyte complexes were placed randomly in the five experimental groups included; GROUP 1: Maturation media (MM) + 100 µM ALA (control), GROUP 2: MM + 100 µM ALA + 50μM α-Tocopherol, GROUP 3: MM + 100 µM ALA + 100μM α-Tocopherol, GROUP 4: MM + 100 µM ALA + 200 μM α-Tocopherol and GROUP 5: MM + 100 µM ALA + 300 μM α-Tocopherol under an atmosphere of 5% CO2 in air at 38.5 °C for 22-24 h. Cumulus expansion and nuclear maturation status was determined (Experiment 1). In experiment 2, oocytes were matured as in experiment 1. The matured oocytes were then fertilized in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h and cultured in synthetic oviductal fluid (SOF) medium to determine effect of α-Linolenic acid (100 µM) and α-Tocopherol in IVM medium on IVC of presumptive zygotes. To study the effect of α-Linolenic acid (100 µM) in IVM media and increasing concentration of α-tocopherol in the culture media on early embryo development (Experiment 3), the presumptive zygotes were randomly distributed into the five experimental groups with increasing concentration of α-tocopherol in culture media. Higher percentage of MII stage oocytes in experiment 1(65.2±2.0), embryos at morula stage in experiment 2 (30.4±1.5) and experiment 3 (22.2±2.0) were obtained. However, overall results for cumulus cell expansion, maturation of oocyte to MII stage and subsequent embryo development among treatments remain statistically similar (P > 0.05). Supplementation of α-tocopherol in maturation media having α-Linolenic acid and/or in embryo culture media did not further enhance in vitro maturation of oocyte or embryo production.

PMID:34932678 | DOI:10.1590/1519-6984.253514

Braz Oral Res. 2021 Dec 17;35:e137. doi: 10.1590/1807-3107bor-2021.vol35.0137. eCollection 2021.

ABSTRACT

BACKGROUND: We evaluated the efficacy of xylitol varnishes on the remineralization of newly erupted permanent and deciduous teeth in vitro and in situ. Human enamel specimens were randomly allocated to 8 groups (n = 15/group). Artificial caries lesions were produced and enamel alterations were quantified by surface/subsurface hardness and transverse microradiography. The blocks were then treated with the following varnishes: DuraphatTM; 20 wt% xylitol (146 μm) varnish; 20 wt% milled xylitol (80 μm) varnish, and placebo varnish, and removed after 6 h of immersion in artificial saliva. The blocks were subjected to pH-cycles for 8 days.

CONCLUSIONS: fifteen subjects wore palatal appliances containing four pre-demineralized and treated enamel specimens, for 5 days. Data were statistically analyzed by ANOVA/Tukey and Kruskal-Wallis/Tukey’s test (p < 0.05). The %SHR in both studies was significantly increased by xylitol and Duraphat™ varnishes when compared to placebo. Considering subsurface remineralization, only the xylitol varnishes were able to significantly reduce the enamel lesion. Xylitol varnishes can be promising alternatives to promote enamel remineralization of newly erupted permanent and deciduous teeth.

PMID:34932666 | DOI:10.1590/1807-3107bor-2021.vol35.0137

Braz Oral Res. 2021 Dec 17;35:e132. doi: 10.1590/1807-3107bor-2021.vol35.0132. eCollection 2021.

ABSTRACT

This study aimed to evaluate the endodontic instrumentation outcomes with asymmetrical files compared to reciprocating and hand files (HFs) in 3D-printed prototypes of upper primary incisors using micro-computed tomography (micro-CT). For this purpose, 50 prototypes were randomly divided (n = 10) according to the instrumentation technique as follows: HFs, a reciprocating file (WaveOne® Gold [WOG]), and three asymmetrical movement files: XP-Endo® Shaper (XPS), XP-Endo® Finisher (XPF), and XP Clean (XPC). The specimens were scanned and, after registration of the baseline and instrumented volumes, changes in the root canal volume (RCV), debris accumulation, removed root material volume (RRMV), non-instrumented areas, and the presence of cracks/perforations were quantified. Data were analyzed by analysis of variance and Student’s t-test, while the effect size was calculated for statistically significant outcomes. All groups showed an increase in RCV after instrumentation (p < 0.05), but this was higher with HFs (p < 0.05). Accumulated debris was higher for WOG and XPS (p < 0.05), but WOG exhibited more in the medium and apical third areas. HFs showed the highest RRMV (p < 0.05), especially at the apical third. The non-instrumented areas were lower for HFs and XPC than for the other systems (p < 0.05). Cracks were present in a few WOG (n = 2) and HF specimens (n = 3) and in this group, one of the cracked specimens and two others showed perforations. The asymmetric systems resulted in conservative dentin removal and fewer cracks/perforations as compared to HFs and a reciprocation file in prototyped primary upper incisors. XPC showed the best compromise between RRMV and non-instrumented areas with a low accumulation of debris.

PMID:34932661 | DOI:10.1590/1807-3107bor-2021.vol35.0132

J Sports Med Phys Fitness. 2021 Dec 21. doi: 10.23736/S0022-4707.21.13171-8. Online ahead of print.

ABSTRACT

INTRODUCTION: The high prevalence of injury among rugby players emphasizes the need for research related to injury risk factors. Physical fitness-related risk factors are likely culprits contributing to both contact and non-contact injuries. Establishing associations between preseason measured physical fitness aspects and injury risk, not only provide players’ baseline fitness parameters but could also identify injury prone players, thereby contributing to injury prevention strategies. Therefore, the objective of this review was to assess and summarize scientific literature related to the association between pre-season measured physical fitness tests and in-season injury among male rugby players.

EVIDENCE ACQUISITION: A systematic review was performed in compliance with the PRISMA 2020 guidelines. This review considered observational, prospective cohort study designs. Studies that included male rugby (rugby union, rugby league, Australian football rules and rugby sevens) players aged 18 years or above from all levels of participation, evaluating the association between physical fitness test outcome and injury, were considered for inclusion. The three-step search strategy aimed at finding both published and unpublished studies in any language. Searched databases included MEDLINE via PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Physiotherapy Evidence Database (PEDro), the Cochrane Controlled Trials Register in the Cochrane Library, ProQuest 5000 International, ProQuest Health and Medical Complete, EBSCO MegaFile Premier, SPORTDiscus with Full Text, SCOPUS and Science Direct. Keywords used were “rugby”, “injury”, “physical fitness”, and “risk factors”. Papers that met the inclusion criteria were assessed by two independent reviewers for methodological validity prior to inclusion in the review using standardized critical appraisal tool for cohort studies from the Joanna Briggs Institute (JBI SUMARI).

EVIDENCE SYNTHESIS: A total of 16 studies were eligible for inclusion in this review. The mean critical appraisal score was 82.63% (SD=17.86). Forty-meter sprint speed was associated with injury in all three (100%) studies that included the test. Inconsistencies in the statistical analysis, however, make comparison difficult. None of the studies that investigated upper (n=1) and/or lower body power (n=3) identified power as a risk factor. Conflicting results were found for the association between strength, flexibility, cardio-respiratory fitness, and injury.

CONCLUSIONS: Identifying factors associated with injury risk is an important step in the injury prevention paradigm. Once identified, players can be screened for risk factors prior to participation in sport. Interventions, based on screening results, which not only improve performance but also decrease players’ risk of sustaining injuries (i.e., physical fitness related risk factors), provide additional incentive for compliance. Overall, this review highlights the inconsistency in testing methods used to gauge specific physical fitness constructs among rugby players, limiting the extent to which comparison of results and pooling of data is possible.

PMID:34931786 | DOI:10.23736/S0022-4707.21.13171-8

Int J Comput Dent. 2021 Dec 21;24(4):385-392.

ABSTRACT

AIM: Innovations in CAD/CAM technology and materials science offer new methodologies for removable prosthodontics. As clinical data are still rare, in vitro performance of both CAD/CAM and comparable conventional materials may help to estimate the clinical outcome.

MATERIALS AND METHODS: Specimens (n = 8 per group) from teeth (CediTEC, SR VivodentCAD, Vitapan), base materials (V-Print dentbase, IvoBase CAD, Paladur), adhesives (CediTEC Primer/Adhesive, IvoBase CAD Bond), and a fully printed specimen (Try-In) were created. All specimens underwent thermal cycling and mechanical loading (TCML): 1,200,000 × 50 N; 2×3000 x 5°C/55°C; H2O. Surviving specimens were loaded to fracture. Statistical tests used were the Shapiro-Wilk test and the Kaplan-Meier survival, with the level of significance set to α = 0.05.

RESULTS: Mean loading cycles until failure varied between 100 and 621,667 cycles. Up to five specimens per group failed during TCML. With one exception, all specimens of the entirely CAD/CAM-fabricated group survived TCML. The log-rank (Mantel-Cox) test showed significantly different (P = 0.000) loading cycles between the systems (chi-square test: 28,247; degree of freedom: 8). Failure of the dentures during TCML was characterized by failure of the denture base (2x), denture tooth (13x), mixed base/tooth (3x) or adhesive between base and tooth (1x).

CONCLUSION: TCML and fracture testing showed different aspects of denture tooth restoration. The results indicated no correlation between fracture force, fracture pattern, and survival cycles. Denture teeth (milled, heat-pressed), bases (milled, printed, pressed), and primer should be matched up to optimize the performance of dentures.

PMID:34931774

Int J Comput Dent. 2021 Dec 21;24(4):393-403.

ABSTRACT

AIM: To investigate retentive forces (RFs) of CAD/CAM-milled and conventionally cast secondary crowns (SCs) after artificial aging in an in vitro study.

MATERIALS AND METHODS: Forty artificial premolars were manufactured and provided with 40 primary crowns (PCs) milled from a high noble metal alloy. SCs were fabricated from the same alloy. Ten SCs were produced with the help of a tactile scanning method (group A), 10 with the help of a photo-optical scan (spray; group B), 10 with the help of a photo-optical scan (acrylic dye; group C), and 10 using a conventional casting technique (group D). Cycles of separation were performed and RFs were measured at baseline and after 5,000 and 10,000 cycles. Surfaces were examined under a scanning electron microscope (SEM). Statistical analysis was conducted at a significance level of P ≤ 0.05.

RESULTS: Group D showed the highest median RFs with respective interquartile ranges (IQRs) – baseline: 7.0(2.5) N; 5,000 cycles: 5.5(2.0) N; 10,000 cycles: 5.4(1.5) N compared with groups A, B, and C – baseline: 5.2(8.4)/3.4(11.3)/1.3(1.5) N; 5,000 cycles: 1.8(0.8)/2.1(1.7)/1.0(1.3) N; 10,000 cycles: 1.9(1.6)/2.4(2.5)/1.0(1.4) N, respectively. In contrast to groups A, B, and C, group D did not gain RF when RF values were compared after 5,000 and 10,000 cycles. The loss of RF between baseline (7.0 N) and after 10,000 cycles (5.4 N) was significant only for group D (P = 0.007), but not for groups A, B, and C.

CONCLUSION: Both CAD/CAM-milled and conventionally cast SCs from a high noble metal alloy can provide sufficient RF after 10,000 cycles of artificial aging. However, groups A, B, and C showed constantly lower RF values compared with group D.

PMID:34931775

Int J Comput Dent. 2021 Dec 21;24(4):375-384.

ABSTRACT

AIM: To evaluate the oral health-related quality of life (OHRQoL) of patients rehabilitated with conventional or 3D-printed implant overdentures.

MATERIALS AND METHODS: A randomized clinical trial (RCT) was designed. Twenty-eight completely edentulous participants were randomly allocated into two equal groups. All participants received two implants with ball attachments. Participants in the control group were rehabilitated with conventionally manufactured polymethyl methacrylate (PMMA) maxillary complete dentures (CDs) and mandibular implant overdentures, while those in the intervention group received digital light processing (DLP)-printed photopolymerizable PMMA maxillary CDs (NextDent) and mandibular implant overdentures. Follow-up appointments were scheduled at 3, 6, and 12 months where Oral Health Impact Profile 19 (OHIPEDENT19) data were used to assess the OHRQoL of the participants. Denture retention was measured using a digital force gauge device.

RESULTS: The OHRQoL values were significantly higher (less improvement) in the conventional overdenture group at 6 months (P = 0.02) and 12 months (P = 0.04). A statistically significant difference was found between the mean retention values of the conventional and 3D-printed overdenture groups. For all the follow-up periods, the mean retention values were higher for the 3D-printed overdenture group (P = 0.001).

CONCLUSION: 3D-printed overdentures may represent an alternative to conventionally fabricated ones. This study represents a stepping stone and proof of concept that support the potential future use of 3D-printed dentures.

PMID:34931773

Int J Comput Dent. 2021 Dec 21;24(4):341-352.

ABSTRACT

An increasing number of accuracy studies on 3D digitizing systems, especially intraoral scanning devices, are being published in scientific and educational journals. The methods, measurement values, and statistical parameters of these studies vary. Certain inconsistencies exist, which lead to difficulty in terms of interpretation and sometimes even questionable conclusions being drawn. These issues make it almost impossible to compare the results of such studies. One aspect inherent in this is the mutable use of basic terms describing the quality of measurement outcomes. A clear definition of such terms and clear instructions as to their respective calculation processes is essential for communication among scientists as well as for reporting measurement results to the dental community. Therefore, the aim of the present guideline is to provide a clear definition of the accuracy, trueness, and precision as the basic terms in the context of digital dentistry. The survey for this guideline included the application of ISO Norms and their expansion to special aspects concerning 3D data acquisition and, in particular, surface meshes. Additionally, the literature was screened to collect approaches, which can be seen as useful for dealing with these terms when performing different kinds of studies.

PMID:34931770

Int J Comput Dent. 2021 Dec 21;24(4):353-362.

ABSTRACT

AIM: The objective of the present study was to assess the accuracy and reproducibility of permanent dentition and dental arch measurements of three digital scanners compared with the gold standard, a physical plaster cast.

MATERIALS AND METHODS: In this cross-sectional study, the following records of 30 patients were used: 1) orthodontic physical plaster study cast (PPSC); 2) digitally scanned physical model (DSPM), 3) direct intraoral model scanned with a Trios color scanner; and 4) direct 3D CBCT digital model. The following 3D measurements were obtained: mesiodistal tooth dimensions; total tooth materials; dental arch perimeters; total arch lengths; and intermolar, interpremolar, and intercanine widths. The measurements on the three digital models were contrasted with those on the PPSC. Differences were tested using a dependent t test for intragroup comparisons. A P value of < 0.05 was considered statistically significant. Intraclass correlation coefficient was used to assess intra- and interexaminer reliability.

RESULTS: Except for the mesiodistal dimensions of the mandibular left central incisors (P < 0.001) and the mandibular intercanine width (P = 0.041), no statistically significant differences were found between the measurements made directly on the PPSC and those on the three digital models. The mean discrepancies between the methods ranged from as low as 0.003 mm to as high as 0.67 mm for the total tooth materials, and as low as 0.01 mm to as high as 0.37 mm for the total arch length. For the transverse dimension, the mean discrepancies ranged from as low as 0.3 mm for the maxillary intercanine width to as high as 0.5 mm for the mandibular intercanine width. However, these significant differences were not considered clinically significant.

CONCLUSIONS: The DSPM, Trios color scanner digital model, and direct 3D CBCT digital model appear to be adequate, reliable, and time-saving alternatives to the PPSC when analyzed using a digital caliper.

PMID:34931771