Tag: statistics

Oral Radiol. 2023 Mar 27. doi: 10.1007/s11282-023-00682-x. Online ahead of print.

ABSTRACT

OBJECTIVES: To investigate possible associations between diffusion-weighted imaging (DWI) parameters derived from a non-Gaussian model fitting and Ki-67 status in patients with oral squamous cell carcinoma (OSCC).

METHODS: Twenty-four patients with newly diagnosed OSCC were prospectively recruited. DWI was performed using six b-values (0-2500). The diffusion-related parameters of kurtosis value (K), kurtosis-corrected diffusion coefficient (D_K), diffusion heterogeneity (α), distributed diffusion coefficient (DDC), slow diffusion coefficient (D_slow), and apparent diffusion coefficient (ADC) were calculated from four diffusion fitting models. Ki-67 status was categorized as low (Ki-67 percentage score < 20%), middle (20-50%), or high (> 50%). Kruskal-Wallis tests were performed between each non-Gaussian diffusion model parameters and Ki-67 grade.

RESULTS: The Kruskal-Wallis tests revealed that multiple parameters (K, ADC, D_k, DDC and D_slow) showed statistically significant differences between the three levels of Ki-67 status (K: p = 0.020, ADC: p = 0.012, D_k: p = 0.027, DDC: p = 0.007 and D_slow: p = 0.026).

CONCLUSIONS: Several non-Gaussian diffusion model parameters and ADC values were significantly associated with Ki-67 status and have potential as promising prognostic biomarkers in patients with OSCC.

PMID:36971988 | DOI:10.1007/s11282-023-00682-x

Ann Surg Oncol. 2023 Mar 27. doi: 10.1245/s10434-023-13375-w. Online ahead of print.

NO ABSTRACT

PMID:36971986 | DOI:10.1245/s10434-023-13375-w

Spine Deform. 2023 Mar 27. doi: 10.1007/s43390-023-00682-1. Online ahead of print.

ABSTRACT

PURPOSE: Intra-operative traction (IOT) has emerged as new modality for improving correction rates in scoliosis since it has the potential to reduce operative time and blood loss in neuromuscular scoliosis (NMS). The aim of this study is to describe the effects of IOT in deformity correction in NMS.

METHODS: The search was conducted in online electronic databases following the PRISMA guidelines. This review included studies on NMS which have described usage of IOT in deformity correction.

RESULTS: Eight studies were included in analysis and review. There was low-to-moderate heterogeneity across the studies (I² – 42.4 to 93.9%). All the studies used cranio-femoral traction for IOT. The final Cobb’s angle in coronal plane was significantly lower in the traction group as compared to the non-traction group (SMD – 0.36 95% CI – 0.71 to 0). There was a trend towards better outcomes in final obliquity (SMD – 0.78 95% CI – 1.64 to 0.09), operative time (SMD – 1.09 95% CI – 2.25 to 0.08) and blood loss (SMD – 0.86 95% CI significantly lower in the traction group as 2.15 to 0.44) but did not reach statistical significance.

CONCLUSION: IOT helped to achieve significant scoliotic curve correction in NMS compared to non-traction group. Despite the overall tendency of improved pelvic obliquity correction, reduced operative time and reduced blood loss as compared to a surgery without the use of IOT, it did not achieve statistical significance. Further studies which are prospective with a larger sample size and focussing on a particular etiology may be conducted which would validate the results.

LEVEL OF EVIDENCE: IV.

PMID:36971963 | DOI:10.1007/s43390-023-00682-1

Environ Sci Pollut Res Int. 2023 Mar 27. doi: 10.1007/s11356-023-26539-8. Online ahead of print.

ABSTRACT

Methylparaben, chloro-methylparaben, and dichloro-methylparaben were evaluated in Allium cepa at 5, 10, 50, and 100 μg/L and in Eisenia fetida at 10 and 100 μg/L. In A. cepa roots, 100 μg/L methylparaben and 50 and 100 μg/L chlorinated methylparabens reduced cell proliferation, caused cellular changes, and reduced cell viability in meristems, which caused a reduction in root growth. Furthermore, they caused drastic inhibition of catalase, ascorbate peroxidase, and superoxide dismutase; activated guaiacol peroxidase and promoted lipid peroxidation in meristematic root cells. In earthworms, after 14 days exposure to the three compounds, there were no deaths, and catalase, ascorbate peroxidase, and superoxide dismutase were not inhibited. However, guaiacol peroxidase activity and lipid peroxidation were observed in animals exposed to dichloro-methylparaben. Soils with dichloro-methylparaben also caused the escape of earthworms. It is inferred that the recurrent contamination of soils with these methylparabens, with emphasis on chlorinated derivatives, can negatively impact different species that depend directly or indirectly on soil to survive.

PMID:36971943 | DOI:10.1007/s11356-023-26539-8

Environ Sci Pollut Res Int. 2023 Mar 27. doi: 10.1007/s11356-023-26564-7. Online ahead of print.

ABSTRACT

There is worldwide concern about how climate change -which involves rising temperatures- may increase the risk of contracting and developing diseases, reducing the quality of life. This study provides new research that takes into account parameters such as land surface temperature (LST), surface urban heat island (SUHI), urban hotspot (UHS), air pollution (SO₂, NO₂, CO, O₃ and aerosols), the normalized difference vegetation index (NDVI), the normalized difference building index (NDBI) and the proportion of vegetation (PV) that allows evaluating environmental quality and establishes mitigation measures in future urban developments that could improve the quality of life of a given population. With the help of Sentinel 3 and 5P satellite images, we studied these variables in the context of Granada (Spain) during the year 2021 to assess how they may affect the risk of developing diseases (stomach, colorectal, lung, prostate and bladder cancer, dementia, cerebrovascular disease, liver disease and suicide). The results, corroborated by the statistical analysis using the Data Panel technique, indicate that the variables LST, SUHI and daytime UHS, NO₂, SO₂ and NDBI have important positive correlations above 99% (p value: 0.000) with an excess risk of developing these diseases. Hence, the importance of this study for the formulation of healthy policies in cities and future research that minimizes the excess risk of diseases.

PMID:36971934 | DOI:10.1007/s11356-023-26564-7

Int Ophthalmol. 2023 Mar 27. doi: 10.1007/s10792-023-02660-y. Online ahead of print.

ABSTRACT

BACKGROUND: To assess and compare the accuracy of 10 intraocular lens (IOL) power calculation formulas after cataract surgery in eyes with an axial length (AL) shorter than or equal to 22.00 mm.

METHODS: A retrospective case series included 100 eyes with an AL ≤ 22.00 mm that underwent uneventful cataract surgery. The refractive prediction error (PE) was calculated using 10 different IOL power calculation formulas: Barrett Universal II, EVO 2.0, Haigis, Hill RBF 2.0, Hoffer Q, Holladay 1 and 2, Kane, SRK/T and SuperLadas. The median absolute prediction error (MedAE ± SD) and mean absolute prediction error (MAE ± SD) were calculated after adjusting the mean prediction error (ME) to 0.

RESULTS: Hoffer Q obtained the lowest MedAE (0.292 D) after adjusting the ME to 0, followed very closely by EVO 2.0 (0.298 D) and Kane (0.300 D). EVO 2.0 and Kane obtained both the lowest MAE after adjusting the ME to 0 (0.386). Differences in MAE among the different formulas were not statistically significant (p > 0.05).

CONCLUSIONS: Our study reflects a tendency of the EVO 2.0 formula and the Kane formula along with the older Hoffer Q formula, to predict more accurately the refractive outcomes in short eyes that undergo cataract phacoemulsification surgery compared to the other formulas, despite this difference could not be statistically proved.

PMID:36971929 | DOI:10.1007/s10792-023-02660-y

Int Ophthalmol. 2023 Mar 27. doi: 10.1007/s10792-023-02685-3. Online ahead of print.

ABSTRACT

PURPOSE: This study aimed to compare the efficacy of topical bevacizumab and motesanib in an experimental corneal neovascularization model, and find the most effective motesanib dose.

MATERIALS AND METHODS: In experiments, 42 Wistar Albino rats were randomly divided into six groups (n = 7). Corneal cauterization was applied to all groups except the group 1. Group 1 did not receive any treatment. Topical dimethylsulfoxide was applied to sham group three times a day(tid). Topical bevacizumab drops (5 mg/ml) were applied to Group 3 tid. Topical motesanib drops with a dose of 2.5, 5, and 7.5 mg/ml were respectively applied in Groups 4, 5, and 6 tid. On the 8th day, corneal photographs of all rats were taken under general anesthesia, and the percentage of corneal neovascular area was calculated. VEGF-A mRNA, VEGFR-2 mRNA, miRNA-21, miRNA-27a, miRNA-31, miRNA-126, miRNA-184, and miRNA-204 were evaluated by the qRT-PCR method in corneas taken after decapitation.

RESULTS: The percentage of corneal neovascularization areas and VEGF-A mRNA expression levels were decreased in all treatment groups compared to group 2 (p < 0.05). VEGFR-2 mRNA levels were found to be statistically significantly decreased in groups 4 and 6 compared to group 2 (p < 0.05). Statistically significant changes were detected in the expression levels of only miRNA-126 among all miRNAs.

CONCLUSION: Motesanib with a dose of 7.5 mg/ml statistically significantly suppressed the VEGFR-2 mRNA level compared with other treatment doses and may be more effective than bevacizumab. Further, miRNA-126 can be used as a proangiogenic marker.

PMID:36971928 | DOI:10.1007/s10792-023-02685-3

Int J Colorectal Dis. 2023 Mar 27;38(1):82. doi: 10.1007/s00384-023-04378-w.

ABSTRACT

PURPOSE: There is not enough information to position medications for the treatment of Crohn’s disease (CD). Therefore, using a network meta-analysis and systematic review, we evaluated the efficacy and safety of combination therapy and infliximab (IFX) monotherapy in CD patients.

METHODS: We identified randomized controlled trials (RCTs) in CD patients who were given IFX-containing combination therapy versus IFX monotherapy. Induction and maintenance of clinical remission were the efficacy outcomes, while adverse events were the safety outcomes. The surface under cumulative ranking (SUCRA) probabilities was used to assess ranking in the network meta-analysis.

RESULTS: In total, 15 RCTs with 1586 CD patients were included in this study. There was no statistical difference between different combination therapies in induction and maintenance of remission. In terms of inducing clinical remission, IFX + EN (SUCRA: 0.91) ranked highest; in terms of maintaining clinical remission, IFX + AZA (SUCRA: 0.85) ranked highest. There was no treatment that was significantly safer than the others. In terms of any adverse events, serious adverse events, serious infections, and infusion/injection-site reactions, IFX + AZA (SUCRA: 0.36, 0.12, 0.19, and 0.24) was ranked lowest for all risks; while IFX + MTX (SUCRA: 0.34, 0.06, 0.13, 0.08, 0.34, and 0.08) was rated lowest for risk of abdominal pain, arthralgia, headache, nausea, pyrexia, and upper respiratory tract infection.

CONCLUSION: Indirect comparisons suggested that efficacy and safety of different combination treatments are comparable in CD patients. For maintenance therapies, IFX + AZA was ranked highest for clinical remission and lowest for adverse events. Further head-to-head trials are required.

PMID:36971914 | DOI:10.1007/s00384-023-04378-w

Med Oncol. 2023 Mar 27;40(5):131. doi: 10.1007/s12032-023-01999-7.

ABSTRACT

Rutin is one of the flavonoids found in fruits and vegetables. The PI3K/AKT/mTOR signaling pathway is critical for the life cycle at the cellular level. In current study, we purposed to demonstrate the antitumoral effect of rutin at different doses through the mTOR-signaling pathway and argyrophilic nucleolar regulatory region. EAC cells were injected subcutaneously into the experimental groups. 25 and 50 mg/kg Rutin were injected intraperitoneally to the animals with solid tumors for 14 days. Immunohistochemical, Real-time PCR and AgNOR analyzes were actualized on the taken tumors. When the rutin given groups and the tumor group were compared, the tumor size increase was detected to be statistically significant (p < 0.05). In immunohistochemical analysis, a significant decrease was encountered in the AKT, mTOR, PI3K and F8 expressions especially in the groups administered 25 mg Rutin, in comparison with the control group (p < 0.05). AgNOR area/nuclear area (TAA/NA) and average AgNOR number were determineted, and statistically important differences were detected between the groups in terms of TAA/NA ratio (p < 0.05). There were significant statistical differences between the mRNA quantity of the PI3K, AKT1 and mTOR genes (p < 0.05). In the in vitro study, cell apoptosis was evaluated with different doses of annexin V and it was determined that a dose of 10 µg/mL Rutin induced apoptosis (p < 0.05). In our study, it was demonstrated in vivo and in vitro that Rutin has an anti-tumor effect on the development of solid tumors formed by both EAC cells.

PMID:36971893 | DOI:10.1007/s12032-023-01999-7

J Dermatol. 2023 Mar 27. doi: 10.1111/1346-8138.16781. Online ahead of print.

ABSTRACT

Infantile hemangiomas (IH) are benign vascular tumors that are common in infancy. They vary in growth, size, location, and depth, and although most lesions are relatively small, approximately one fifth of patients have multiple lesions. Risk factors for IH include female sex, low birth weight, multiple gestation, preterm birth, progesterone therapy, and family history, but the mechanism that causes multiple lesions is unclear. We hypothesized that blood cytokines are involved as a cause of multiple IHs, and tried to prove this using sera and membrane arrays from patients with single and multiple IHs. Serum samples were obtained from five patients with multiple lesions and four patients with a single lesion, none of which had received any treatment. Serum levels of 20 cytokines were measured using human angiogenesis antibody membrane array. Four of the 20 cytokines (bFGF, IFN-γ, IGF-I, and TGF-β1) were higher in the patients with multiple lesions than in those with single lesion, with statistically significant difference (p < 0.05). Notably, signal for IFN-γ was evident in all cases with multiple IHs, but was absent in cases with single IH. Although not significant, there was mild correlation between IFN-γ and IGF-I (r = 0.64, p = 0.065), and between IGF-I and TGF-β1 (r = 0.63, p = 0.066). bFGF levels were strongly and significantly correlated with the number of lesions (r = 0.88, p = 0.0020). In conclusion, blood cytokines could act as a cause of multiple IHs. This is a pilot study with a small cohort, so further large-scale studies are necessary.

PMID:36971143 | DOI:10.1111/1346-8138.16781