Tag: statistics

Cell Mol Biol (Noisy-le-grand). 2022 Aug 31;68(8):202-209. doi: 10.14715/cmb/2022.68.8.34.

ABSTRACT

Myeloproliferative neoplasms have a high prevalence and genetic mutations play a role in their occurrence. Determination of these mutations can be valuable in the screening, diagnosis, and treatment of patients. Therefore, this study was conducted to investigate the mutation of JAK2, CALR, and MPL genes as diagnostic and prognostic biomarkers in patients with myeloproliferative neoplasms in the Kurdistan region of Iraq. This case-control study was conducted in 2021 on 223 patients with myeloproliferative neoplasm referred to Hiwa Sulaymaniyah Cancer Hospital. The data were collected from three groups of Polycythemia Vera (PV) patients (70 people), Essential Thrombocythemia (ET) (50 people), and Primary Myelofibrosis (PMF) (103 people) by sampling for JAK2, CALR, and MPL gene mutation tests and demographic and clinical information have been collected through examination. The data were analyzed by SPSS v. 23 software and descriptive and chi-square statistical tests. The study included 223myeloproliferative neoplasms (MPN) patients. JAK2 V617F mutation was detected mostly in PV patients and CALR and MPL mutations in ET and PMF patients and this mutation difference was significant in prognosis and disease diagnosis. An association between JAK 2 mutation and splenomegaly was also demonstrated. Considering the lack of a definitive diagnostic method in myeloproliferative disease, the results of this study showed that molecular studies, including JAK2 V617F, CALR, and MPL mutations and other hematological tests can be useful and effective in the diagnosis of MPN. In addition, it is necessary to pay attention to new diagnostic methods.

PMID:36800830 | DOI:10.14715/cmb/2022.68.8.34

Cell Mol Biol (Noisy-le-grand). 2022 Aug 31;68(8):92-95. doi: 10.14715/cmb/2022.68.8.16.

ABSTRACT

In order to analysis of the mechanisms regulating EBNA1 killing of EBV associated B cell tumors, preparations were first made for EBV-associated B cells, and the cells were subsequently transformed. The killing effect of ebna1-28 T cells on EBV-positive B cell lymphoid tumor cells was detected using the FACS method. SF rats were also selected to analyze the inhibitory effect of ebna1-28t on transplanted tumors in nude mice with EBV-positive B cell lymphoma. Results showed that compared with the untransfected group, the expression of EBNA1 was higher in the empty plasmid SFG group, the recombinant plasmid rv-ebna1/car group compared with the empty plasmid SFG group, and the expression of EBNA1 was higher in the untransfected group compared with the empty plasmid SFG group, which was statistically significant (P < 0.05); As shown in Figure 1, in vitro studies found that, compared to the untransfected group, the empty plasmid SFG group, the recombinant plasmid rv-ebna1/car group showed better killing efficacy on Raji cells, and the recombinant plasmid rv-ebna1/car group showed better killing efficacy on Raji cells compared to the empty plasmid SFG group; The tumor volumes of the rats in group C were larger compared with those in groups A and B, and the tumor volumes of the rats in group A were smaller compared with those in group B. The tumor volumes of the rats in group C were larger compared with those of the rats in the three groups (P<0.05). In group C, the cells were more severely invaded, and the nuclei were damaged. In group B, cell invasion in tissues was mild in the nucleus. The infection of cells in the tissues of rats in group A was better compared to groups B and C. In vitro experiments found that inhibition of EBNA1 was able to kill EBV-positive B cell lymphoid tumor cells effectively. Animal experiments found that ebna1-28t was able to shrink the volume as well as tumor weight of transplanted tumors in nude mice with EBV-positive B cell lymphoma and played a better inhibitory role.

PMID:36800829 | DOI:10.14715/cmb/2022.68.8.16

Cell Mol Biol (Noisy-le-grand). 2022 Aug 31;68(8):102-104. doi: 10.14715/cmb/2022.68.8.18.

ABSTRACT

Heart failure is one of the common cardiovascular diseases, and digoxin is required in the list of drug treatments. Considering the positive effect of this drug on heart failure, unfortunately, its therapeutic and toxic serum levels are different and very close to each other in different people. This study aimed to investigate the digoxin serum level in heart failure patients. For this purpose, we examined 32 patients with heart failure and digoxin users in this cross-sectional descriptive study. Some important factors involved in determining digoxin toxicity, such as age, gender, creatinine, creatinine clearance, cardiac output, urea, potassium, calcium, and digoxin levels, were measured. Statistical analysis showed that digoxin serum level increases with age (p<0.01). The increase in digoxin serum level was related to urea, creatinine, and potassium serum levels (p<0.01). In general, it seems that to prevent the increase of digoxin serum level and poisoning with it, it is necessary to continuously control the serum level of this drug in the form of serum measurement or according to its clearance.

PMID:36800827 | DOI:10.14715/cmb/2022.68.8.18

Cell Mol Biol (Noisy-le-grand). 2022 Aug 31;68(8):145-150. doi: 10.14715/cmb/2022.68.8.26.

ABSTRACT

Endometriosis is one of the women’s aggressive but benign diseases, formed by the presence of endometrial glands and stroma outside the uterine cavity. Various genes, including the GATA2 gene, are involved in the pathogenesis of endometriosis. Since this disease affects patients’ quality of life, this study was conducted to investigate the effect of nurses’ supportive and educational care on the quality of life of endometriosis patients and its role in GATA2 gene expression. For this purpose, 45 patients with endometriosis participated in this research, a semi-experimental before-and-after study. The instrument used was demographic information and quality of life questionnaires affiliated with Beckman Institute, which were completed in two stages before and after implementing patient training and support sessions. The real-time PCR technique was also used to evaluate the expression level of the GATA2 gene after obtaining endometrial tissue from patients before and after the intervention. Finally, the received information was analyzed using SPSS software and statistical tests. Based on the obtained results, the average quality of life score before the intervention was 51.73±13.91, and after the intervention was 60.46±13.80 (P<0.001). Also, in all four dimensions of quality of life, patients’ average scores increased after the intervention compared to before the intervention. Still, this difference was significant only in the two dimensions of physical and mental health (P<0.001). GATA2 gene expression before intervention was 0.35 ± 0.13 among endometriosis patients. After the intervention, its amount reached about three times, i.e., 0.96 ± 0.32, which showed a significant difference between the two groups at the 5% probability level. In general, the results of this research confirmed the positive effect of educational and support programs in improving the quality of life of breast cancer patients. Therefore, it is suggested to design and implement such programs in a broader manner and based on patients’ educational and support needs.

PMID:36800818 | DOI:10.14715/cmb/2022.68.8.26

Minerva Anestesiol. 2023 Feb 21. doi: 10.23736/S0375-9393.23.16956-2. Online ahead of print.

ABSTRACT

BACKGROUND: Pain following open reduction and internal fixation of distal radius fracture (DRF) can be significant. This study compared the intensity of pain up to 48 hours after volar plating for DRF, associated to either an ultrasound guided distal nerve block (DNB) or surgical site infiltration (SSI).

METHODS: In this prospective single blind randomized study, 72 patients scheduled for DRF surgery under 1.5% lidocaine axillary block were allocated to receive, at the end of surgery, either an ultrasound-guided median and radial nerves block with ropivacaine 0.375% (DNB) performed by the anesthesiologist or a SSI with the same drug regimen, performed by the surgeon. Primary outcome was the duration between analgesic technique (H0) and pain reappearance (Numerical Rating Scale (NRS 0-10)>3). Secondary outcomes were the quality of analgesia, the quality of sleep, the magnitude of motor blockade, and the patient satisfaction. The study was built on a statistical hypothesis of equivalence.

RESULTS: Fifty-nine patients were included in the final per-protocol analysis (DNB=30, SSI=29). Time to reach NRS>3 was (in median [95%CI]) 267 min [155;727] and 164 min [120;181] respectively after DNB and SSI (difference=103 min [-22;594] – rejection of equivalence hypothesis). Pain intensity throughout the 48 hours, quality of sleep, opiate consumption, motor blockade and patient satisfaction was not significantly different between groups.

CONCLUSIONS: Although DNB provides a longer analgesia than SSI, both techniques gave comparable level of pain control during the first 48 hours after surgery, without any difference in the incidence of side effects or patient satisfaction.

PMID:36800809 | DOI:10.23736/S0375-9393.23.16956-2

Minerva Anestesiol. 2023 Feb 21. doi: 10.23736/S0375-9393.22.16913-0. Online ahead of print.

ABSTRACT

BACKGROUND: The prokinetic effect of metoclopramide promotes gastric emptying and decreases stomach capacity. The aim of the present study was to assess the efficacy of metoclopramide in reducing gastric contents and volume using gastric point-of-care ultrasonography (PoCUS) in parturients females prepared for elective Cesarean section under general anesthesia.

METHODS: A total of 111 parturient females were randomly allocated to one of two groups. The intervention group (Group M; N.=56) received 10 mg metoclopramide diluted in 10 mL 0.9% normal saline. The control group (Group C; N.=55): received 10 mL 0.9% normal saline. The cross-sectional area and volume of stomach contents were measured using ultrasound before and one hour after the administration of metoclopramide or saline.

RESULTS: Statistically significant differences in mean antral cross-sectional area and gastric volume were observed between the two groups (P<0.001). Group M had significantly lower rates of nausea and vomiting compared to the control group.

CONCLUSIONS: Metoclopramide decreases gastric volume, reduces postoperative nausea and vomiting, and may lower the risk of aspiration when used as premedication before obstetric surgery. Preoperative gastric PoCUS has utility in objectively assessing stomach volume and contents.

PMID:36800808 | DOI:10.23736/S0375-9393.22.16913-0

Ital J Dermatol Venerol. 2023 Feb 21. doi: 10.23736/S2784-8671.23.07364-4. Online ahead of print.

ABSTRACT

BACKGROUND: Xerosis is an extremely common condition, especially in the elderly population. It is the most common cause of pruritus in the older adult. Since xerosis is generally caused by a lack of epidermal lipids, the use of leave-on skin care products is the mainstay treatment. The aim of this open prospective analytical observational study was to investigate the clinical and self-reported hydrating efficacy of a moisturizer formulation containing a synergy between amino-inositol and urea (INOSIT-U 20) in patients with psoriasis and xerosis.

METHODS: Twenty-two patients with psoriasis successfully treated with biologic therapy, and who presented xerosis, were recruited. Each patient was instructed to apply the topical with a frequency of two applications per die on the identified skin area. Corneometry values and a VAS itch questionnaire were measured at baseline (T0) and after 28 days (T4). To evaluate the cosmetic efficacy, the volunteers also completed a self-assessment questionnaire.

RESULTS: Comparing Corneometry values at T0 and T4, a statistically significant increase value was observed in the area subjected to topical treatment (P<0.0001). A significant decrease in itch (P=0.001) was also observed. Moreover, the patients’ ratings of the cosmetic properties of the moisturizer showed significant confirmation rates.

CONCLUSIONS: This study provides preliminary evidence that INOSIT-U20 provides a good hydrating effect on xerosis, further reducing self-reported itch.

PMID:36800804 | DOI:10.23736/S2784-8671.23.07364-4

Stomatologiia (Mosk). 2023;102(1):94-97. doi: 10.17116/stomat202310201194.

ABSTRACT

OBJECTIVE: The aim of the study is determination of the effectiveness of technologies for predicting the progressive course of dental caries in pregnant women.

MATERIAL AND METODS: We examined 511 pregnant women aged 18-40 years with dental caries (304 were included in a main group, 207 were enrolled in controls), who were assessed the DMFT index sequentially in the 1st, 2nd and 3rd trimesters of pregnancy. The prognosis of recurrence of dental caries was determined by the method of two-stage clinical and laboratory prognosis.

RESULTS: The prevalence of dental caries in the main group was 89.1% (271 out of 304 patients), in the control group – 87.9% (182 out of 207 patients). In the third trimester of pregnancy 36.2% of women in the main group had a recurrence of caries (vs 43.0% in the control group). Early examination of patients in the 1st trimester of pregnancy, followed by monitoring of the state of the organs and tissues of the mouth, made it possible to achieve timely treatment of dental caries and prevent its recurrence. In the third trimester of pregnancy, the DMFT-index in the dispensary group, in contrast to the control group, was statistically significantly (p<0.05) lower by 12.3%, which indicated the effective use of the proposed monitoring.

CONCLUSION: The use of a system for providing dental treatment and preventive care in the form of screening, dynamic forecasting and assessment of the risk of recurrence of caries in pregnant women with dental caries and a high risk of its progression makes it possible to stop the development of this process and ensure the preservation of dental health.

PMID:36800794 | DOI:10.17116/stomat202310201194

Stomatologiia (Mosk). 2023;102(1):86-93. doi: 10.17116/stomat202310201186.

ABSTRACT

PURPOSE OF THE STUDY: For the first time distinctions of molecular composition of the dental biofilm at the stages of exo- and endogeneous caries prevention were studied for persons with different cariogenic conditions involving synchrotron molecular spectroscopy techniques.

MATERIAL AND METHODS: The samples of the dental biofilm collected from participants of the research were studied at the different stages of experiment. The studies of molecular composition of the biofilms were employed involving the equipment set in the Infrared Microspectroscopy (IRM) laboratory of Australian synchrotron.

RESULTS: Basing on the data obtained by synchrotron infrared spectroscopy with Fourier transform as well as using the calculations of the ratios between organic and mineral components and also statistical analysis of the data we could estimate the changes proceeding in the molecular composition of dental biofilm in a dependence of homeostasis conditions in the oral cavity at the stages of exo- and endogeneous caries prevention.

CONCLUSION: Observed changes in the values of phosphate/protein/lipid, phosphate/mineral and phospholipid/lipid ratios as well as the presence of statistically significant intra- and intergroup in these coefficients mean that mechanisms of adsorption for the ions, compounds and molecular complexes incoming from the oral fluid into the dental biofilm at the stage of exo-/endogeneous caries prevention are different for the patients in normal condition and for those ones with the developing caries.

PMID:36800793 | DOI:10.17116/stomat202310201186

Stomatologiia (Mosk). 2023;102(1):60-65. doi: 10.17116/stomat202310201160.

ABSTRACT

OBJECTIVE: The aim of the study is to determine the X-ray morphometric parameters of the neck of the mandible for the rational choice of fixing elements during osteosynthesis.

MATERIAL AND METHODS: Based on 145 computed tomography scans of the mandible, the parameters of the upper and lower borders, the area and thickness of the neck of the mandible were studied. The anatomical boundaries of the neck were determined using the classification of A. Neff (2014). The parameters of the neck of the mandible were studied depending on the shape of the mandible ramus, gender, age and preservation of dentition.

RESULTS: The sizes of morphometric parameters of the neck of the mandible prevail in men. Statistically significant differences were found between the sizes of the neck of the mandible in men and women in the width of the lower border, area, and thickness of bone tissue. It was revealed that there are statically significant differences between hypsiramimandibular, orthoramimandibular and platyramimandibular forms in the following parameters: the width of the lower and upper borders, the middle of the neck, the area of bone tissue. When comparing the morphometric parameters of the neck of the articular process, there were no statistically significant differences between the age groups (p>0.05), and no differences were found between the groups identified by the degree of preservation of the dentition (p>0.05).

CONCLUSION: Morphometric parameters of the neck of the mandible have individual variability, statically significant differences depending on the sex and shape of the mandibular ramus. The obtained results on the width, thickness, and area of the bone tissue of the neck of the mandible will help in clinical practice to rationally choose the length of screws; the size, number, and shape of titanium mini-plates to achieve the requirements of stable functional osteosynthesis.

PMID:36800787 | DOI:10.17116/stomat202310201160