Tag: statistics

BMC Public Health. 2022 Aug 16;22(1):1559. doi: 10.1186/s12889-022-13959-3.

ABSTRACT

BACKGROUND: Uncontrolled diabetes is an important public health problem that endangers the quality of life of patients. Promoting self-management through well-planned training is an essential strategy to control diabetes effectively. This study aimed to examine the effects of a training program based on social cognitive theory (SCT) on self-management behavior, glycemic index, and quality of life among patients with type 2 diabetes mellitus.

METHODS: This is a quasi-experimental study with a pretest-posttest design. The statistical population included 106 adults with type 2 diabetes mellitus assigned to the intervention and control groups [n₁ = n₂ = 53], who received services from two urban health centers. A multi-method, SCT-based training program consisting of six 60-80-min sessions was run, followed by 2-3 follow-up home visits [once a month for each participant] for the intervention group. The data were collected before and three months after the intervention and were analyzed in SPSS 19.

RESULTS: Before the intervention, there was no significant difference between the two groups regarding the main variables. After the intervention, there was a significant increase in the intervention group’s mean scores of knowledge, self-efficacy, social support, outcome expectations, self-regulation, self-management behavior, glycemic index, and quality of life. There were no significant changes in these constructs in the control group after the intervention. The regression analysis results indicated that social cognitive theory and self-management could explain the variance in quality of life [adjusted R-squared = 0.476].

CONCLUSIONS: The findings support the effectiveness of the multi-method, SCT-based educational intervention in improving self-management behaviors, glycemic index, and quality of life among patients with type 2 diabetes mellitus. It is suggested that the quality of type 2 diabetes care programs should be promoted. However, further research is needed to evaluate the long-term outcomes.

PMID:35974352 | DOI:10.1186/s12889-022-13959-3

Infect Dis Poverty. 2022 Aug 17;11(1):90. doi: 10.1186/s40249-022-01013-8.

ABSTRACT

BACKGROUND: Dengue (DENV), chikungunya (CHIKV) and Zika virus (ZIKV), are mosquito-borne viruses of medical importance in most tropical and subtropical regions. Vector control, primarily through insecticides, remains the primary method to prevent their transmission. Here, we evaluated insecticide resistance profiles and identified important underlying resistance mechanisms in populations of Aedes aegypti and Ae. albopictus from six different regions in Cameroon to pesticides commonly used during military and civilian public health vector control operations.

METHODS: Aedes mosquitoes were sampled as larvae or pupae between August 2020 and July 2021 in six locations across Cameroon and reared until the next generation, G1. Ae. aegypti and Ae. albopictus adults from G1 were tested following World Health Organization (WHO) recommendations and Ae. aegypti G0 adults screened with real time melting curve qPCR analyses to genotype the F1534C, V1016I and V410L Aedes kdr mutations. Piperonyl butoxide (PBO) assays and real time qPCR were carried out from some cytochrome p450 genes known to be involved in metabolic resistance. Statistical analyses were performed using Chi-square test and generalized linear models.

RESULTS: Loss of susceptibility was observed to all insecticides tested. Mortality rates from tests with 0.25% permethrin varied from 24.27 to 85.89% in Ae. aegypti and from 17.35% to 68.08% in Ae. albopictus. Mortality rates for 0.03% deltamethrin were between 23.30% and 88.20% in Ae. aegypti and between 69.47 and 84.11% in Ae. albopictus. We found a moderate level of resistance against bendiocarb, with mortality rates ranging from 69.31% to 90.26% in Ae. aegypti and from 86.75 to 98.95% in Ae. albopictus. With PBO pre-exposure, we found partial or fully restored susceptibility to pyrethroids and bendiocarb. The genes Cyp9M6F88/87 and Cyp9J10 were overexpressed in Ae. aegypti populations from Douala sites resistant to permethrin and deltamethrin. Cyp6P12 was highly expressed in alphacypermethrin and permethrin resistant Ae. albopictus samples. F1534C and V1016I mutations were detected in A. aegypti mosquitoes and for the first time V410L was reported in Cameroon.

CONCLUSIONS: This study revealed that Ae. aegypti and Ae. albopictus are resistant to multiple insecticide classes with multiple resistance mechanisms implicated. These findings could guide insecticide use to control arbovirus vectors in Cameroon.

PMID:35974351 | DOI:10.1186/s40249-022-01013-8

BMC Public Health. 2022 Aug 16;22(1):1557. doi: 10.1186/s12889-022-13945-9.

ABSTRACT

BACKGROUND: Taxes on discretionary foods and sugar-sweetened beverages have emerged as a strategy for health promotion. Between 2018-2019, the Bermuda government introduced a phased tax on imported sugar-sweetened beverages, confectionery, products containing cocoa and pure sugar, and eliminated import duties on select healthy food items. The aim of this study was to conduct an mixed methods evaluation of perceptions of the tax among the general population and key stakeholders.

METHODS: We conducted a survey of the general population (N = 400), and semi-structured interviews with key informants (N = 14) from the government, food and beverage, and health sectors to understand awareness, acceptability, and perceived impact of the tax after implementation. Survey data was analysed using thematic analysis, summary statistics, and Chi-squared tests. Key informant interviews were analysed using the framework method.

RESULTS: General population respondents had high awareness of the sugar tax (94%) but low awareness of the healthy food subsidy (32%). Most respondents (67%) felt the tax was not an appropriate way to motivate healthier consumption due to beliefs the tax would not be effective (44%), and because of the high price of healthy food (20%). However, nearly half (48%) reported consuming fewer taxed products, primarily for health reasons but also motivated by price increases. Key informants indicated there was high awareness but limited understanding of the tax policy. Informants expressed support for taxation as a health promotion strategy, conditional on policy implementation. The lack of clear price differentiation between taxed and un-taxed products and the absence of accompanying health education were key factors believed to affect the impact of the tax. No informants were aware of use of tax revenues for health purposes and tax revenue was reportedly re-directed to other priorities after implementation.

CONCLUSIONS: There was high awareness, but limited acceptability of the Bermuda sugar tax as implemented. Clarity in the tax policy, appropriateness of the tax mechanism, and use of revenue in alignment with the tax aim are critical components for acceptance. The absence of complementary education and health promotion affected acceptance and may limit potential health impacts. The lessons learned in Bermuda can inform similar policies in other settings.

PMID:35974346 | DOI:10.1186/s12889-022-13945-9

BMC Anesthesiol. 2022 Aug 16;22(1):262. doi: 10.1186/s12871-022-01805-3.

ABSTRACT

BACKGROUND: Remimazolam is a newer benzodiazepine with properties of rapid onset, short duration of action, and fast recovery. Our study was to evaluate the effects of different doses of remimazolam combined with alfentanil in colonoscopic polypectomy.

METHODS: One hundred twenty patients were randomly divided into four groups: alfentanil and propofol (AP) group, alfentanil and remimazolam 0.1 mg/kg (AR1 group), 0.15 mg/kg (AR2 group), or 0.2 mg/kg (AR3 group). Patients in the four groups received alfentanil 10 μg/kg, followed by propofol 2 mg/kg and three dosages of remimazolam. Modified Observer’s Assessment of Alertness and Sedation (MOAA/S) scale, heart rate (HR), oxygen saturation (SpO₂), respiratory rate (RR), bispectral index (BIS) values and mean arterial pressure (MAP) were collected at intervals of 5 min and analyzed at different time points: before anesthesia (T0), 5 min (T1), 10 min (T2), 15 min after anesthesia (T3) and at the end of surgery (T4). The average MAP was calculated utilizing the average of all MAP values. The primary outcome was the success rate of sedation. Secondary outcomes included time to full alert and adverse events.

RESULTS: The success rate of sedation was 100% among the four groups. The incidence of hypotension was significantly decreased (all P < 0.05) and the average MAP was higher in AR1-AR3 groups than AP group (all P < 0.001). None of the patients developed bradycardia or hypertension during surgery in all study groups. BIS values were higher (all P < 0.001) and the time to full alert was statistically shorter in AR1-AR3 groups (all P < 0.05) compared with the AP group. The MOAA/S score in AR1 was higher than AR2 (P < 0.05) and the AR3 group (P < 0.05) at T1 and BIS values in the AR1 group were significantly higher than AR3 group (P < 0.05) at T4.

CONCLUSIONS: Remimazolam combined with alfentanil have a non-inferior sedative effect than propofol during the colonoscopic polypectomy. Moreover, this combination of two short-acting drugs might be a safer alternative.

TRIAL REGISTRATION: The clinical trial was registered on (16/05/2021, ChiCTR2100046492).

PMID:35974309 | DOI:10.1186/s12871-022-01805-3

BMC Bioinformatics. 2022 Aug 16;23(Suppl 8):340. doi: 10.1186/s12859-022-04875-9.

ABSTRACT

BACKGROUND: Cells progressing from an early state to a developed state give rise to lineages in cell differentiation. Knowledge of these lineages is central to developmental biology. Each biological lineage corresponds to a trajectory in a dynamical system. Emerging single-cell technologies such as single-cell RNA sequencing can capture molecular abundance in diverse cell types in a developing tissue. Many computational methods have been developed to infer trajectories from single-cell data. However, to our knowledge, none of the existing methods address the problem of determining the existence of a trajectory in observed data before attempting trajectory inference.

RESULTS: We introduce a method to identify the existence of a trajectory using three graph-based statistics. A permutation test is utilized to calculate the empirical distribution of the test statistic under the null hypothesis that a trajectory does not exist. Finally, a p-value is calculated to quantify the statistical significance for the presence of trajectory in the data.

CONCLUSIONS: Our work contributes new statistics to assess the level of uncertainty in trajectory inference to increase the understanding of biological system dynamics.

PMID:35974302 | DOI:10.1186/s12859-022-04875-9

Ophthalmol Ther. 2022 Aug 16. doi: 10.1007/s40123-022-00556-1. Online ahead of print.

ABSTRACT

INTRODUCTION: This study aimed to evaluate the efficacy and safety of a new-generation intense pulsed light (IPL) device in improving the symptoms and signs of meibomian gland dysfunction (MGD)-related dry eye, and compare it with a traditional IPL device.

METHODS: This multicenter randomized controlled trial enrolled 132 patients with MGD-related dry eye from two centers. Patients were randomly assigned into the new-generation IPL (Eyesis) group or traditional IPL (E-Eye) group, and then blinded to receive treatment on days 0 and 7. Ocular Surface Disease Index (OSDI), tear meniscus height (TMH), tear breakup time (TBUT), corneal fluorescein staining (CFS), Schirmer test, and meibomian gland signs were evaluated on days 0, 7, and 14. The primary outcome was defined as the effective rate of treating MGD at day 14. Any adverse events were recorded for safety assessment. Intergroup comparisons and non-inferiority analysis were performed. p values less than 0.05 were considered statistically significant.

RESULTS: Basic information showed no significant difference between treatment groups. The intergroup difference of the effective rate was – 1.7% in the left eye and 1.6% in right eye, verifying the non-inferiority of the Eyesis device (p = 0.927). Significant improvements in OSDI, TBUT, Schirmer test, TMH, CFS, and meibomian gland signs were observed in Eyesis group on days 7 and 14 (all p < 0.05). Compared to the E-Eye group, the Eyesis group achieved more significant improvements in OSDI, TBUT, Schirmer test, TMH, and meibum quality (all p < 0.05). There was no significant difference in the incidences of adverse events between groups (p = 1.000).

CONCLUSIONS: The new-generation IPL was effective and safe in relieving the symptoms and signs of MGD-related dry eye, exhibiting a non-inferior effective rate compared to the traditional IPL. Additionally, Eyesis showed more clinical benefits over E-Eye in alleviating symptoms, increasing tear film stability and improving meibomian gland function.

PMID:35974296 | DOI:10.1007/s40123-022-00556-1

Obes Surg. 2022 Aug 16. doi: 10.1007/s11695-022-06240-4. Online ahead of print.

ABSTRACT

PURPOSE: The development of Laparoscopic Linear Endostaplers (LLES) is crucial in minimally invasive approaches in bariatric surgery, but there have been very few published studies comparing 6-row LLES in Laparoscopic Sleeve Gastrectomy (LSG). The objective of this study was to compare two 6-row LLES in LSG.

METHODS: A total of 60 patients were prospectively randomized to undergo LSG with either Medtronic Endo GIA™ Tri-Staple technology (MTS) or AEON ™ Endostapler(Lexington Medical) LLES. The measured parameters included patient demographics, comorbidity indices, LLES and specimen characteristics, postoperative symptoms, hospital stay, and total adverse events (AEs). Intraoperative bleeding was evaluated using five laparoscopic and corresponding endoscopic images of staple line before clip application, compared with a 1-5 Visual Analogue Scale (VAS), assessed by an independent bariatric surgeon who was blinded to the LLES used. Images of all cases were reviewed on the same day to increase test-retest reliability.

RESULTS: Both groups were similar in patient demographics. Compared to MTS, AEON LLES group had significantly lower bleeding VAS scores in 4/5 laparoscopic images (pre-pyloric: 1.7 ± 0.7 vs. 2.36 ± 0.76, p = 0.0007, mid-sleeve: 1.46 ± 0.62 vs. 1.86 ± 0.68, p = 0.019, proximal sleeve: 1.6 ± 0.77 vs. 2.0 ± 0.83, p = 0.038, gastro-esophageal junction: 1.43 ± 0.67 vs. 1.86 ± 0.77, p = 0.014) and 3/5 endoscopic images (pre-pyloric: 1.56 ± 0.56 vs. 2.36 ± 0.76, p = 0.006, incisura: 1.66 ± 0.54 vs. 2.0 ± 0.52, p = 0.021, mid-sleeve: 1.63 ± 0.49 vs. 2.0 ± 0.45, p = 0.005). There was no statistical difference in other parameters.

CONCLUSION: Both devices were equally safe and effective in terms of LLES and specimen characteristics, patient symptoms, hospital stay, and AEs. Bleeding VAS scores were significantly lower, favoring the AEON LLES.

PMID:35974292 | DOI:10.1007/s11695-022-06240-4

Eur J Drug Metab Pharmacokinet. 2022 Aug 16. doi: 10.1007/s13318-022-00787-4. Online ahead of print.

ABSTRACT

BACKGROUND AND OBJECTIVES: Alirocumab is a cholesterol-lowering monoclonal antibody targeting proprotein convertase subtilisin kexin type 9 (PCSK9) indicated in the prevention of cardiovascular risk and exhibiting target-mediated drug disposition (TMDD). The aim of this work was to develop an integrated pharmacokinetic-pharmacodynamic model to describe the interaction of alirocumab with PCSK9 and its impact on the evolution of low-density lipoprotein cholesterol (LDL-C) levels and explore labeling specification for subpopulations.

METHODS: Using data collected from nine phase I/II/III clinical studies (n = 527, subcutaneous or intravenous administration), a TMDD model considering the quasi-steady-state approximation was developed to characterize the interaction dynamics of alirocumab and PCSK9, combined with an indirect pharmacodynamic model describing the inhibition of LDL-C by PCSK9 in a one-step approach using nonlinear-mixed effects modeling. A “full fixed effects modeling” strategy was implemented to quantify parameter-covariate relationships.

RESULTS: The model captures the interaction between alirocumab and its target PCSK9 and how this mechanism drives LDL-C depletion, with an estimation of the associated between-subject variability of model parameters and the quantification of clinically relevant parameter-covariate relationships. Co-administration of statins was found to increase the central volume of distribution of alirocumab by 1.75-fold (5.6 L versus 3.2 L) and allow for a 14% greater maximum lipid-lowering effect (88% versus 74%), highlighting the synergy of action between anti-PCSK9 therapeutic antibodies and statins toward lowering LDL-C plasma levels. Baseline levels of PCSK9 were found to be related to the amplitude of LDL-C variations by increasing the concentration of free PCSK9 necessary to reach half its capacity of inhibition of LDL-C degradation.

CONCLUSION: The maximum effect of alirocumab is achieved when free PCSK9 concentration is close to zero, as seen mostly after 150 mg every 2 weeks (Q2W) or 300 mg every 4 weeks (Q4W), indicating that there would be no additional clinical benefit of increasing the dose higher than these recommended dosing regimens.

PMID:35974290 | DOI:10.1007/s13318-022-00787-4

Environ Sci Pollut Res Int. 2022 Aug 16. doi: 10.1007/s11356-022-22528-5. Online ahead of print.

ABSTRACT

The accurate division of three zones of coal spontaneous combustion in the goaf plays a vital role for coal fire prevention. Based on the O₂ and CO volume fraction acquired from in situ test, this paper first fits the linear equation (characteristic equation) of O₂ volume fraction with the length of the goaf. Then a cloud map of the kernel density distribution of O₂ and CO volume fraction and the length of the goaf was drawn. According to the cloud map of CO, the distribution interval of CO volume fraction can be obtained, in which 0-100 ppm is the acceptable range, and greater than 100 ppm is the dangerous range, which can be used as a critical indicator for early warning of coal spontaneous combustion. According to the kernel density distribution cloud map of O₂ volume fraction, there are 3 peaks of the kernel density of O₂ volume fraction. According to the difference test, the 3 goaf lengths (characteristic lengths) corresponding to the 3 kernel density peaks are determined to be 12 m, 34 m, and 59 m, respectively. The characteristic O₂ volume fractions are obtained by substituting characteristic lengths into the characteristic equation, which are 17.9%, 13.6%, and 8.9%. Different from the traditional dividing method, the characteristic O₂ volume fractions and characteristic lengths divide the goaf into four areas: the heat dissipation zone, the first oxidation zone, the second oxidation zone, and the asphyxiation zone. The results of this study could refine the division of coal spontaneous combustion dangerous areas, reflect the dynamic change process of coal spontaneous combustion dangerous areas, and improve the efficiency of coal spontaneous combustion prevention.

PMID:35974273 | DOI:10.1007/s11356-022-22528-5

Environ Sci Pollut Res Int. 2022 Aug 16. doi: 10.1007/s11356-022-22515-w. Online ahead of print.

ABSTRACT

Geographical information system data has been used in forest fire risk zone mapping studies commonly. However, forest fires are caused by many factors, which cannot be explained only by geographical and meteorological reasons. Human-induced factors also play an important role in occurrence of forest fires, and these factors depend on various social and economic conditions. This article aims to prepare a fire risk zone map by using a data set consisting of 11 human-induced factors, a natural factor, and temperature, which is one of the risk factors that determine the conditions for the occurrence of forest fires. Moreover, k-means clustering algorithm, which is an artificial intelligence method, was employed in preparation of the fire risk zone map. Turkey was selected as the study area because there are social and economic variations among its regions. Thus, the regional forest directorates in Turkey were separated into four clusters as extreme-risk zone, high-risk zone, moderate-risk zone, and low-risk zone. Also, a map presenting these risk zones were provided. The map reveals that, in general, the western and southwestern coastal areas of Turkey are at high risk of forest fires. On the other hand, the fire risk is relatively low in the northern, central, and eastern areas.

PMID:35974271 | DOI:10.1007/s11356-022-22515-w