Tag: statistics

J Obstet Gynaecol Res. 2021 Aug 10. doi: 10.1111/jog.14980. Online ahead of print.

ABSTRACT

OBJECTIVE: To examine the prevalence of female sexual dysfunction (FSD) in gynecologic cancer survivors.

METHODS: A cross-sectional observation survey recruited women aged 18-65 years old who were diagnosed with gynecologic cancers and underwent surgery, radiation, chemotherapy, or combined modalities. Data were collected from September 2019 to March 2020 by the Thai version of the female sexual function index questionnaire to define FSD. The survey contained six domains: desire, arousal, lubrication, orgasm, satisfaction, and pain.

RESULTS: Of the 98 women included in the study, the mean age was 48.7 ± 10.6 years old. Two-third (67.3%) of the participants was premenopausal, had been diagnosed with ovarian cancer, and had early-stage gynecologic cancers. Fifty-eight of 98 (59.2%) participants were sexually active in the past 4 weeks before the survey. Among those, 89.6% had FSD. The median scores in the FSD group were significantly lower than those in the non-FSD group in all six domains, namely, desire, arousal, lubrication, orgasm, satisfaction, and pain (p < 0.05). There was no statistically significant difference observed between participants with and without FSD in terms of age group, cancer-related data, or sexual behavior data, except participants who had good/excellent relationships with their partners, which impacted FSD (p = 0.01).

CONCLUSIONS: This study discovered the high prevalence (89.6%) of hidden issue of “female sexual dysfunction” in Thai gynecologic cancer survivors. The good/excellent relationships with their partners affected the FSD who were sexually active in the past 4 weeks before the survey.

PMID:34376017 | DOI:10.1111/jog.14980

Nature. 2021 Aug 10. doi: 10.1038/s41586-021-03855-y. Online ahead of print.

ABSTRACT

Genome-wide association studies have uncovered thousands of common variants associated with human disease, but the contribution of rare variation to common disease remains relatively unexplored. The UK Biobank (UKB) contains detailed phenotypic data linked to medical records for approximately 500,000 participants, offering an unprecedented opportunity to evaluate the impact of rare variation on a broad collection of traits^1,2. Here, we studied the relationships between rare protein-coding variants and 17,361 binary and 1,419 quantitative phenotypes using exome sequencing data from 269,171 UKB participants of European ancestry. Gene-based collapsing analyses revealed 1,703 statistically significant gene-phenotype associations for binary traits, with a median odds ratio of 12.4. Furthermore, 83% of these associations were undetectable via single variant association tests, emphasizing the power of gene-based collapsing analysis in the setting of high allelic heterogeneity. Gene-phenotype associations were also significantly enriched for loss-of-function-mediated traits and approved drug targets. Finally, we performed ancestry-specific and pan-ancestry collapsing analyses using exome sequencing data from 11,933 UKB participants of African, East Asian, or South Asian ancestry. Together, our results highlight a significant contribution of rare variants to common disease. Summary statistics are publicly available through an interactive portal ( http://azphewas.com/ ).

PMID:34375979 | DOI:10.1038/s41586-021-03855-y

Econ Hum Biol. 2021 Jul 30;43:101050. doi: 10.1016/j.ehb.2021.101050. Online ahead of print.

ABSTRACT

Hiring discrimination towards (former) burnout patients has been extensively documented in the literature. To tackle this problem, it is important to understand the underlying mechanisms of such unequal hiring opportunities. Therefore, we conducted a vignette experiment with 425 genuine recruiters and jointly tested the potential stigma against job candidates with a history of burnout that were mentioned earlier in the literature. We found candidates revealing a history of burnout elicit perceptions of requiring work adaptations, likely having more unpleasant collaborations with others as well as diminished health, autonomy, ability to work under pressure, leadership capacity, manageability, and learning ability, when compared to candidates with a comparable gap in working history due to physical injury. Led by perceptions of a reduced ability to work under pressure, the tested perceptions jointly explained over 90 % of the effect of revealing burnout on the probability of being invited to a job interview. In addition, the negative effect on interview probability of revealing burnout was stronger when the job vacancy required higher stress tolerance. In contrast, the negative impact of revealing burnout on interview probability appeared weaker when recruiters were women and when recruiters had previously had personal encounters with burnout.

PMID:34375926 | DOI:10.1016/j.ehb.2021.101050

Early Hum Dev. 2021 Aug 2;161:105436. doi: 10.1016/j.earlhumdev.2021.105436. Online ahead of print.

ABSTRACT

OBJECTIVE: To describe the inter-observer reliability of the General Movement Assessment (GMA) among a sample of infants at high-risk of cerebral palsy (CP) among raters with various levels of experience.

METHODS: Video assessments of 150 high-risk infants at 10-15 weeks corrected age were rated by three Prechtl GMA-certified observers with varied experience using the assessment. Videos were scored based on temporal organization of fidgety movements (FMs), presence of abnormal FMs, or absence of FMs. Inter-observer agreements were analyzed with Gwet’s AC1 statistic.

RESULTS: We found fair to moderate agreement when subcategories of normal FMs (continuous and intermittent) were included (AC₁ = 0.32-0.57) and moderate to near perfect agreement when normal categories of FMs were combined (AC₁ = 0.60-0.95). Reliability was higher among observers with more experience using the GMA (AC₁ = 0.57-0.98) than the observer with less experience (AC₁ = 0.32-0.61).

CONCLUSIONS: Caution may be warranted when the GMA is used to differentiate “continuous and intermittent” FMs temporal organization. The GMA is highly reliable among experienced raters when comparing normal FMs to other FMs categorizations.

PMID:34375936 | DOI:10.1016/j.earlhumdev.2021.105436

Biomed Phys Eng Express. 2021 Aug 10. doi: 10.1088/2057-1976/ac1c51. Online ahead of print.

ABSTRACT

MR-guided radiotherapy (MRgRT) systems provide excellent soft tissue imaging immediately prior to and in real time during radiation delivery for cancer treatment. However, 2D cine MRI often has limited spatial resolution due to high temporal resolution. This work applies a super resolution machine learning framework to 3.5mm pixel edge length, low resolution (LR), sagittal 2D cine MRI images acquired on a MRgRT system to generate 0.9mm pixel edge length, super resolution (SR), images originally acquired at 4 frames per second (FPS). LR images were collected from 50 pancreatic cancer patients treated on a ViewRay MR-LINAC. SR images were evaluated using three methods. 1) The first method utilized intrinsic image quality metrics for evaluation. 2) The second used relative metrics including edge detection and structural similarity index (SSIM). 3) Finally, automatically generated tumor contours were created on both low resolution and super resolution images to evaluate target delineation and compared with DICE and SSIM. Intrinsic image quality metrics all had statistically significant improvements for SR images versus LR images, with mean (± 1 SD) BRISQUE scores of 29.65±2.98 and 42.48±0.98 for SR and LR, respectively. SR images showed good agreement with LR images in SSIM evaluation, indicating there was not significant distortion of the images. Comparison of LR and SR images with paired high resolution (HR) 3D images showed that SR images had a mean (± 1 SD) SSIM value of 0.633±0.063 and LR a value of 0.587±0.067 (p << 0.05). Contours generated on SR images were also more robust to noise addition than those generated on LR images. This study shows that super resolution with a machine learning framework can generate high spatial resolution images from 4fps low spatial resolution cine MRI acquired on the ViewRay MR-LINAC while maintaining tumor contour quality and without significant acquisition or post processing delay.

PMID:34375963 | DOI:10.1088/2057-1976/ac1c51

Food Chem. 2021 Aug 2;367:130748. doi: 10.1016/j.foodchem.2021.130748. Online ahead of print.

ABSTRACT

The development of a collaborative study as a requirement for the preparation of a laboratory reference material candidate is reported in this paper. The evaluation was performed by 13 laboratories invited to quantify the calcium, potassium, magnesium, phosphorus, copper, iron, manganese and zinc; 8 of them presented results for all the analytes under investigation. The data were statistically analyzed by applying the z-score robust technique as recommended by ISO Guide 35. For the potassium element, laboratories 4 and 13 presented questionable results. Laboratory 5 proved to be unsatisfactory for calcium and zinc. ANOVA-PCA and DD-SIMCA were also applied to evaluate stability and interlaboratory studies results, respectively. It has been demonstrated that multivariate data analysis can be successfully applied as an alternative method to the recommendations made by ISO 13528 and ISO Guide 35 with defined confidence intervals.

PMID:34375894 | DOI:10.1016/j.foodchem.2021.130748

Atherosclerosis. 2021 Aug 4;332:24-32. doi: 10.1016/j.atherosclerosis.2021.08.004. Online ahead of print.

ABSTRACT

BACKGROUND AND AIMS: Dyslipidemias are common among patients with chronic kidney disease (CKD) and are a major risk factor for cardiovascular disease. This study aimed to investigate the association between early-stage CKD and new-onset dyslipidemia for each lipid profile.

METHODS: This nationwide longitudinal study included data from the Japan Specific Health Checkups (J-SHC) Study. New-onset dyslipidemia was indicated by hypertriglyceridemia (High-TG; ≥150 mg/dL), hyper-LDL cholesterolemia (High-LDL-C; ≥140 mg/dL), or hypo-HDL chelesterolemia (Low-HDL-C; <40 mg/dL) levels according to the guideline of Japan Atherosclerosis Society, or High-TG/HDL-C ratio (≥3.5) which was a good predictor of atherosclerosis. The incidence of new-onset dyslipidemia was compared between participants with and without CKD. Survival curves were used to analyze the incidence of each dyslipidemia.

RESULTS: Of 289,462 participants with a median follow-up period of 3 years, the incidence of High-TG, High-LDL-C, Low-HDL-C, and High-TG/HDL-C ratios were 64.4/1000 person-years, 83.1/1000 person-years, 14.5/1000 person-years, and 39.6/1000 person-years, respectively. The adjusted hazard ratios (95% confidence intervals) for High-TG, High-LDL-C, Low-HDL-C, and High-TG/HDL-C ratio were 1.09 (1.05-1.13), 0.99 (0.95-1.04), 1.12 (1.05-1.18), and 1.14 (1.09-1.18), respectively, in CKD participants as compared to non-CKD participants. Decreased eGFR and presence of proteinuria were independently associated with higher risks for new-onset of High-TG, Low-HDL-C, and High-TG/HDL-C ratios.

CONCLUSIONS: CKD was associated with a higher risk of new-onset High-TG, Low-HDL-C, and High-TG/HDL-C ratios, but not High-LDL-C, in the general population. These CKD-specific lipid abnormalities may explain the residual risk for CKD-related cardiovascular disease.

PMID:34375910 | DOI:10.1016/j.atherosclerosis.2021.08.004

Tissue Cell. 2021 Jun 23;73:101585. doi: 10.1016/j.tice.2021.101585. Online ahead of print.

ABSTRACT

The involvement of endometrial IGF-1R/IGF-1/Bcl-2 pathways and the potential regulatory effects of exogenously administrated melatonin on this expression is investigated in the experimental PCOS model in the present study. Thirty-two 6-8 week old Sprague Dawley rats were divided into four groups: the Sham Control Group (1% CMC/day by oral gavage [o.g.]); the Melatonin Group (2 mg/kg/day melatonin by subcutaneous administration [s.c.]); the Experimental PCOS Group (1 mg/kg/day Letrozole by o.g.); and the Experimental PCOS + Melatonin Group (1 mg/kg/day Letrozole by o.g. and 2 mg/kg/day melatonin by s.c. administration). Vaginal smear samples were taken from the 14th day to the end of the experiment for colpocytological measurements. At the end of the 21 day experimental period, uterine tissues were taken; Hematoxylin-Eosin histochemical, IGF-1R/IGF-1/Bcl-2, PCNA immuno-histochemical stainings and western blot analyses were performed for related antibodies. All of the data was supported statistically. The epithelium of endometrium lost its single-layer structure in some parts, separation was observed between the epithelium and the basal membrane junction, intracellular edema was found in the uterine glands by the polycystic ovary-induction. Also this induction increased the expression of IGF-1R/IGF-1, Bcl-2, and PCNA proteins. Morphological degenerations returned to its normal appearance generally by the melatonin administrations and melatonin also regulated the increased expression of endometrial IGF-1R/IGF-1/Bcl-2 and PCNA pathways. It is concluded that additional studies are needed, using melatonin as a supporting agent may be appropriate in cases of PCOS.

PMID:34375824 | DOI:10.1016/j.tice.2021.101585

Chemosphere. 2021 Aug 4;286(Pt 2):131782. doi: 10.1016/j.chemosphere.2021.131782. Online ahead of print.

ABSTRACT

The current study was dedicated to finding the effect of soil amendments (biochar and compost) on plants belonging to Poaceae and Fabaceae families. Plants selected for the phytoremediation experiment included wheat (Triticum aestivum), maize (Zea mays), white clover (Trifolium repens), alfalfa (Medicago sativa), and ryegrass (Lolium multiflorum). The physiological and microbial parameters of plants and soil were affected negatively by the 4 % TPHs soil contamination. The studied physiological parameters were fresh and dried biomass, root and shoot length, and chlorophyll content. Microbial parameters included root and shoot endophytic count. Soil parameters included rhizospheric CFUs and residual TPHs. Biochar with wheat, maize, and ryegrass (Fabaceae family) and compost with white clover and alfalfa (Poaceae family) improved plant growth parameters and showed better phytoremediation of TPHs. Among different plants, the highest TPH removal (68.5 %) was demonstrated by ryegrass with compost, followed by white clover with biochar (68 %). Without any soil amendment, ryegrass and alfalfa showed 59.55 and 35.21 % degradation of TPHs, respectively. Biochar and compost alone removed 27.24 % and 6.01 % TPHs, respectively. The interactive effect of soil amendment and plant type was also noted for studied parameters and TPHs degradation.

PMID:34375825 | DOI:10.1016/j.chemosphere.2021.131782

Int J Hyg Environ Health. 2021 Aug 7;237:113821. doi: 10.1016/j.ijheh.2021.113821. Online ahead of print.

ABSTRACT

The German Environmental Survey (GerES) is a population-representative, cross-sectional study on environmental exposures of the general population of Germany. GerES has repeatedly been conducted since 1985 by the German Environment Agency (UBA) in close collaboration with the Health Interview and Examination Surveys of the Robert Koch Institute (RKI). In the German Environmental Survey for Children and adolescents 2014-2017 (GerES V) pollutants and other environmental stressors were measured in human samples as well as in the homes of 3- to 17-year-old children and adolescents. Interviews were conducted about health-related behaviors and living conditions. The GerES V basic program encompassed examinations of whole blood, blood plasma, morning urine and drinking water samples, measurements of ultrafine particles and noise levels, comprehensive standardized interviews, and self-administrated questionnaires. Additional modules on volatile organic compounds and aldehydes, particulate matter (PM_2.5) in indoor air, organic compounds in drinking water and pollutants in house dust were conducted in subsamples. Potential GerES V participants were identified and attained by the RKI from those participants who were examined and interviewed for the cross-sectional component of the second follow-up to the German Health Interview and Examination Survey for Children and Adolescents (KiGGS Wave 2). The gross sample of GerES V comprised 3031 children and adolescents of which 2294 finally took part in the survey. This equals a total response rate of 75.7 %. Response rates varied, depending on region, type of municipality, age and sex, from 66.0 % to 78.3 %. By calculating individual case weights, discrepancies due to sample design and non-response between the GerES V sample and the whole population could be considered in statistical analysis. Therefore, the representativeness of the GerES V results with regard to age, sex, community size and region was assured.

PMID:34375847 | DOI:10.1016/j.ijheh.2021.113821