J Med Virol. 2022 Mar 13. doi: 10.1002/jmv.27721. Online ahead of print.
ABSTRACT
Crimean Congo hemorrhagic fever (CCHF) is an acute viral infection that can cause death. The detection of host transcriptome is important for understanding differences in pathogenesis of the disease. Long noncoding RNAs (lncRNAs) regulate gene expression in different biological processes. They have also emerged as a key molecules for therapeutic target. We investigated the lncRNA gene expression profiles by utilizing the microarray for the first time in CCHF. LncRNAs were determined by the comparisons between case-control, fatal case-control, and fatal case-nonfatal case. Quantitative polymerase chain reaction (qPCR) was applied to validate the microarray results of some lncRNAs. In our study, 39 lncRNAs (5 downregulated, 34 upregulated) were found to be significantly regulated in the cases when compared to the controls (p<0,05; FC≥2). One hundred ten lncRNAs exhibited a statistically significant difference between fatal cases and controls. FER1L4, ECRP and LOC100133669 are important lncRNAs in both case and fatal case groups compared with controls. These lncRNAs may be considered as important therapeutic targets for the CCHF in further studies. This article is protected by copyright. All rights reserved.
PMID:35285033 | DOI:10.1002/jmv.27721
