Nevin Manimala Statistics

Encapsulation of phenolic acids into cyclodextrins: a global statistical analysis of the effects of pH, temperature and concentrations on binding constants measured by ACE methods

Electrophoresis. 2022 Jun 11. doi: 10.1002/elps.202200075. Online ahead of print.


Affinity capillary electrophoresis was used for the simultaneous measurement of the


values and of the binding constants relative to the encapsulation of naturally occurring phenolic acids (rosmarinic and caffeic acids) with cyclodextrins. A thorough study as a function of pH and temperature was coupled to a detailed statistical analysis of the resulting experimental data. A step-by-step curve fitting process was sufficient for obtaining individual binding constant for each experimental condition, but the influence of temperature remained unclear. A quantitative and qualitative gain was then obtained by supplementing this initial analysis with a global multi-parameters optimization. This lead to the estimation of both entropy and enthalpy of reaction, and to the full description of the binding reactions as a function of pH and temperature. The encapsulation was shown to be very sensitive to pH and temperature, with optimal complexation occurring at low pH and low temperature, gaining up to a factor 3 by cooling from 36°C to 15°C, and up to a factor 10 by lowering the pH from 7 to 2. This article is protected by copyright. All rights reserved.

PMID:35689604 | DOI:10.1002/elps.202200075

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