J AOAC Int. 2022 Jun 23:qsac068. doi: 10.1093/jaoacint/qsac068. Online ahead of print.
ABSTRACT
BACKGROUND: The 3M™ Molecular Detection Assay 2-Salmonella method is based on real time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2 – Salmonella was approved as AOAC® Performance TestedSM Certificate No. 091501 and as AOAC Official Method of AnalysisSM 2016.01.
OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2 – Salmonella for detection of Salmonella in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size.
METHODS: Matrix studies in dried cannabis and hemp flowers followed procedures outlined in 3M Molecular Detection Assay 2 – Salmonella Product Instructions and Standard Method Performance Requirements (SMPRs®) for Detection of Salmonella species in Cannabis and Cannabis Products (AOAC SMPR 2020.002). The method was evaluated at low, high, and non-contaminated levels.
RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2 – Salmonella results and the SMPR 2020.002 recommended cultural confirmations.
CONCLUSION: This study demonstrates that the 3M Molecular Detection Assay 2 – Salmonella is a reliable method for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower.
HIGHLIGHT: The 3M Molecular Detection Assay 2 – Salmonella method is suitable for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower.
PMID:35737412 | DOI:10.1093/jaoacint/qsac068
