J AOAC Int. 2022 Jun 23:qsac069. doi: 10.1093/jaoacint/qsac069. Online ahead of print.
ABSTRACT
BACKGROUND: The 3M™ Molecular Detection Assay 2 – STEC Gene Screen (stx) method is based on gene amplification by the use of real time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Shiga toxin gene (stx1 and/or stx2) from Shiga toxin-producing Escherichia coli (STEC) in enriched products. The 3M Molecular Detection Assay 2 – STEC Gene Screen (stx) was approved as AOAC® Performance Tested MethodSM Certificate No. 071903.
OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2 – STEC Gene Screen (stx) method for detection of STECs in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size.
METHODS: Testing followed procedures outlined in 3M Molecular Detection Assay 2 – STEC Gene Screen (stx) Product Instructions and Standard Method Performance Requirements (SMPRs®) for Detection of Shiga toxin-producing Escherichia coli in Cannabis and Cannabis Products (AOAC SMPR 2020.012). The method was evaluated at low, high, and non-inoculated levels.
RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2 – STEC Gene Screen (stx) results and the SMPR 2020.012 recommended cultural confirmations.
CONCLUSION: This study provides data that demonstrate the 3M Molecular Detection Assay 2 – STEC Gene Screen (stx) is a reliable method for the rapid and specific detection of STECs in dried cannabis flower and dried hemp flower.
HIGHLIGHT: The 3M Molecular Detection Assay 2 – STEC Gene Screen (stx) method is suitable for the rapid and specific detection of STECs in dried cannabis flower and dried hemp flower.
PMID:35737394 | DOI:10.1093/jaoacint/qsac069