Nevin Manimala Statistics

Flowcytometric Ratios of Immune Cells in the Prognosis and Staging of Acute Biliary Pancreatitis

J Coll Physicians Surg Pak. 2022 Aug;32(8):1004-1008. doi: 10.29271/jcpsp.2022.08.1004.


OBJECTIVE: To evaluate several lymphocyte subtypes with various parameters that can be applied easily and give fast results for their roles in evaluating the stage and prognosis of acute biliary pancreatitis.

STUDY DESIGN: Case-control study.

PLACE AND DURATION OF STUDY: The Emergency Department of Istanbul Training and Research Hospital, and Gaziosmanpasa Research and Training Hospital, Turkey, between June 2020 and April 2021.

METHODOLOGY: Patients, who admitted to the Emergency Department with acute pancreatitis and treated after hospitalisation, were included in the study. The patients were divided into three groups; mild, moderately severe and severe, according to the 2012 revised Atlanta classification. Hematocrit, creatinine, potassium, sodium values, and flow cytometry ratios of lymphocyte, monocyte, CD4+, CD8+, and regulatory T cells were measured and the difference between the groups were evaluated. Their results were compared with healthy volunteers.

RESULTS: A total of 53 persons including 40 with acute pancreatitis (14 mild, 14 moderately severe, and 12 in the severe pancreatitis groups) and 13 healthy volunteers, were included in the study. The average age of the studied participants was 50.9 ±13.42 years, 43.3% males and 56.7% females. Leukocyte values, lymphocyte rates, hematocrit rates, age, hospital staying duration, creatinine, potassium values, CD4+, and CD3+ lymphocyte rates were found to be different at a statistically significant level between the groups.

CONCLUSION: High leukocyte, low lymphocyte, high hematocrit, advanced age, elevated creatinine, elevated potassium, low CD4+ T lymphocyte, low CD3+ T lymphocyte, and low lymphocyte/monocyte ratio were identified as poor prognostic indicators.

KEY WORDS: Acute pancreatitis, Flow cytometry, Regulatory T cell, Atlanta.

PMID:35932123 | DOI:10.29271/jcpsp.2022.08.1004

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