Nevin Manimala Statistics

Palmitate-mediated disruption of the endoplasmic reticulum decreases motility of intracellular vesicles

Biophys J. 2023 Mar 3:S0006-3495(23)00154-6. doi: 10.1016/j.bpj.2023.03.001. Online ahead of print.


Essential cellular processes such as metabolism, protein synthesis, and autophagy require the intracellular transport of membrane-bound vesicles. The importance of the cytoskeleton and associated molecular motors for transport is well-documented. Recent research has suggested that the endoplasmic reticulum (ER) may also play a role in vesicle transport through a tethering of vesicles to the ER. We use single particle tracking fluorescence microscopy and a Bayesian changepoint algorithm to characterize vesicle motility in response to the disruption of the ER, actin, and microtubules. This high-throughput changepoint algorithm allows us to efficiently analyze thousands of trajectory segments. We find that palmitate-mediated disruption of the ER leads to a significant decrease in vesicle motility. A comparison with the disruption of actin and microtubules shows that disruption of the ER has a significant impact on vesicle motility, greater than the disruption of actin. Vesicle motility was dependent on cellular region, with greater motility in the cell periphery than the perinuclear region, possibly due to regional differences in actin and the ER. Overall, these results suggest that the ER is an important factor in vesicle transport.

PMID:36869590 | DOI:10.1016/j.bpj.2023.03.001

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