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Endothelin A Receptor Inhibition Increases Cutaneous Nitric Oxide-Dependent Vasodilation Independent of Superoxide in Non-Hispanic Black Young Adults

J Appl Physiol (1985). 2023 Mar 9. doi: 10.1152/japplphysiol.00739.2022. Online ahead of print.


Young non-Hispanic Black adults have reduced microvascular endothelial function compared with non-Hispanic White counterparts, but the mechanisms are not fully elucidated. The purpose of this study was to investigate the effect of endothelin-1 A receptor (ETAR) and superoxide on cutaneous microvascular function in young non-Hispanic Black (n=10) and White (n=10) adults. Participants were instrumented with four intradermal microdialysis fibers: 1) lactated Ringer’s (control), 2) 500 nM BQ-123 (ETAR antagonist), 3) 10 μM tempol (superoxide dismutase mimetic), and 4) BQ-123 + tempol. Skin blood flow was assessed via laser-Doppler flowmetry (LDF), and each site underwent rapid local heating from 33°C to 39°C. At the plateau of local heating, 20 mM L-NAME [nitric oxide (NO) synthase inhibitor] was infused to quantify NO-dependent vasodilation. Data are mean ± standard deviation. NO-dependent vasodilation was decreased in non-Hispanic Black compared with non-Hispanic White young adults (p < 0.01). NO-dependent vasodilation was increased at BQ-123 sites (73 ± 10% NO) and at BQ-123 + tempol sites (71 ± 10 %NO) in non-Hispanic Black young adults compared with control (53 ± 13 %NO, p = 0.01). Tempol alone had no effect on NO-dependent vasodilation in non-Hispanic Black young adults (63 ± 14 %NO, p = 0.18). NO-dependent vasodilation at BQ-123 sites was not statistically different between non-Hispanic Black and White (80 ± 7 %NO) young adults (p = 0.15). ETAR contribute to reduced NO-dependent vasodilation in non-Hispanic Black young adults independent of superoxide, suggesting a greater effect on NO synthesis rather than NO scavenging via superoxide.

PMID:36892887 | DOI:10.1152/japplphysiol.00739.2022

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