Nevin Manimala Statistics

Functional non-parametric mixed effects models for cytotoxicity assessment and clustering

Sci Rep. 2023 Mar 11;13(1):4075. doi: 10.1038/s41598-023-31011-1.


A multitude of natural and synthetic chemicals are present in our environment.Through the study of a compound’s cytotoxicity, researchers can carefully set regulations regarding how much of a certain chemical in the ambient environment is tolerable. In the past, research has focused on point measurements such as the LD50. Instead, we consider entire time-dependent cellular response curves through the application of functional mixed effects models. We identify differences in such curves corresponding to the chemical’s mode of action-i.e. how the compound attacks human cells. Through such analysis, we identify curve features to be used for cluster analysis via application of both k-means and self organizing maps. The data is analyzed by making use of functional principal components as a data driven basis and separately by considering B-splines for identifying local-time features. Our analysis can be used to drastically speed up future cytotoxicity research.

PMID:36906619 | DOI:10.1038/s41598-023-31011-1

By Nevin Manimala

Portfolio Website for Nevin Manimala