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X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression

J Pak Med Assoc. 2023 Mar;73(3):494-499. doi: 10.47391/JPMA.5312.


OBJECTIVE: To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation.

METHODS: The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5.

RESULTS: The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y.

CONCLUSION: There was a significant role of glycosylation in radiation therapy for lung cancer.

PMID:36932748 | DOI:10.47391/JPMA.5312

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