Nevin Manimala Statistics

Mapping Kenyon cell inputs in Drosophila using dye electroporation

STAR Protoc. 2023 Oct 20;4(4):102478. doi: 10.1016/j.xpro.2023.102478. Online ahead of print.


Here, we describe a technique for charting the inputs of individual Kenyon cells in the Drosophila brain. In this technique, a single Kenyon cell per brain hemisphere is photo-labeled to visualize its claw-like dendritic terminals; a dye-filled electrode is used to backfill the projection neuron connected to each claw. This process can be repeated in hundreds of brains to build a connectivity matrix. Statistical analyses of such a matrix can reveal connectivity patterns such as random input and biased connectivity. For complete details on the use and execution of this protocol, please refer to Hayashi et al. (2022).1.

PMID:37864788 | DOI:10.1016/j.xpro.2023.102478

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