Trop Parasitol. 2025 Jan-Jun;15(1):21-24. doi: 10.4103/tp.tp_10_24. Epub 2025 Apr 5.
ABSTRACT
CONTEXT: Laboratory diagnosis of strongyloidiasis is most often based on the detection of antibodies against antigens in an enzyme-linked immunosorbent assay (ELISA).
AIMS: The aim of the study was to validate the efficacy of the B-cell epitope peptide antigens of fatty acid and retinal binding protein in using antibodies against Strongyloides stercoralis.
SETTINGS AND DESIGN: Patient samples were identified by an anonymous code and collected from volunteers at Suranaree University of Technology Hospital, Thailand.
SUBJECTS AND METHODS: The synthesized peptides and cell lysate of Strongyloides ratti were used as antigens in an in-house ELISA format. A total of 78 serum samples were collected from patients infected with S. stercoralis, other parasites, and healthy persons.
STATISTICAL ANALYSIS USED: The receiver operating characteristic curve was used to assess the diagnostic performance of peptide antigens and analysis of variance was utilized to examine differences in peptide reactivity between groups.
RESULTS: The peptide-1 and cell lysate of S. ratti could clearly differentiate serums among infected and noninfected groups. The peptide-1, peptide-2 of S. stercoralis, and lysate of S. ratti antigen had been measured to reveal sensitivities at about 75.00%, 50.00%, and 75.00% with 75.86%, 86.21%, and 89.66% specificities, respectively.
CONCLUSIONS: These results could be useful for routine clinical laboratory diagnostic testing and possibly apply for surveillance investigations in the future.
PMID:40433641 | PMC:PMC12105782 | DOI:10.4103/tp.tp_10_24
