Nat Biotechnol. 2026 Apr 22. doi: 10.1038/s41587-026-03084-6. Online ahead of print.
ABSTRACT
Single-cell RNA sequencing (scRNA-seq) analyses typically focus on alignment and differential gene expression, being blind to other transcriptome variation. Here we present sc-SPLASH for statistics-first, reference-free discovery on barcoded scRNA-seq and spatial transcriptomics; its independent BKC submodule optimizes barcoded data preprocessing and is approximately 50-fold faster than UMI-tools. sc-SPLASH discovers secreted repeat proteins in immune-like cells of sponge (Spongilla; missing from the reference) and tunicate (Ciona).
PMID:42020848 | DOI:10.1038/s41587-026-03084-6
