Category: Statistics

Environ Sci Pollut Res Int. 2022 Jul 1. doi: 10.1007/s11356-022-21775-w. Online ahead of print.

NO ABSTRACT

PMID:35776313 | DOI:10.1007/s11356-022-21775-w

Aging Clin Exp Res. 2022 Jul 1. doi: 10.1007/s40520-022-02173-1. Online ahead of print.

ABSTRACT

BACKGROUND: There is a paucity of the literature on the relationship between frailty and excess mortality due to the COVID-19 pandemic.

METHODS: The entire community-dwelling adult population of Ontario, Canada, as of January 1st, 2018, was identified using the Cardiovascular Health in Ambulatory Care Research Team (CANHEART) cohort. Residents of long-term care facilities were excluded. Frailty was categorized through the Johns Hopkins Adjusted Clinical Groups (ACG® System) frailty indicator. Follow-up was until December 31st, 2020, with March 11th, 2020, indicating the beginning of the COVID-19 pandemic. Using multivariable Cox models with patient age as the timescale, we determined the relationship between frailty status and pandemic period on all-cause mortality. We evaluated the modifier effect of frailty using both stratified models as well as incorporating an interaction between frailty and the pandemic period.

RESULTS: We identified 11,481,391 persons in our cohort, of whom 3.2% were frail based on the ACG indicator. Crude mortality increased from 0.75 to 0.87% per 100 person years from the pre- to post-pandemic period, translating to ~ 13,800 excess deaths among the community-dwelling adult population of Ontario (HR 1.11 95% CI 1.09-1.11). Frailty was associated with a statistically significant increase in all-cause mortality (HR 3.02, 95% CI 2.99-3.06). However, all-cause mortality increased similarly during the pandemic in frail (aHR 1.13, 95% CI 1.09-1.16) and non-frail (aHR 1.15, 95% CI 1.13-1.17) persons.

CONCLUSION: Although frailty was associated with greater mortality, frailty did not modify the excess mortality associated with the pandemic.

PMID:35776284 | DOI:10.1007/s40520-022-02173-1

Clin Rheumatol. 2022 Jul 1. doi: 10.1007/s10067-022-06266-0. Online ahead of print.

ABSTRACT

INTRODUCTION: Elastic band exercise not only improves muscle strength and elasticity, increases efficiency, balance, and quality of life but also decreases the injury risk. This study was conducted to determine the effect of elastic band exercise on pain, kinesiophobia, functional, and psychological status after total knee arthroplasty.

METHODS: This study was conducted out between October 2019 and April 2020 as a randomized controlled experimental study with a total of 60 patients. Data collection tools in this study included a descriptive information form, visual analog scale, Tampa Kinesiophobia, WOMAC Osteoarthritis Index, and Beck Depression Inventory.

RESULTS: Before intervention, the total score of VAS, WOMAC, Tampa Kinesiophobia, and Beck Depression showed no statistically significant difference between intervention group and control group. However, 4 weeks after intervention observed a statistically significant difference between the intervention and control groups in terms of VAS (U = 10,000; p < 0.001), WOMAC (U = 0.00; p < 0.001), Tampa Kinesiophobia (U = 221.5; p = 0.001), and Beck Depression means score (U = 112.5; p < 0.001). In addition, 4 weeks after the intervention, joint range and walking test assessments were statistically significant between the intervention and control groups (p < 0.001).

CONCLUSION: The results of the research showed that elastic band exercise had a positive effect on psychological and functional capacity.

TRIAL REGISTRATION: This study is registered in the Clinical Trial Registry (registration number NCT04981106) Key points • Patients with total knee arthroplasty require rehabilitation exercise to avoid physical function weakness. • Elastic band exercise significantly reduces patients’ fear of movement and depression. • Elastic band exercise activities facilitate significant physical motion function. • Elastic band exercise inspires the will of patients to engage in home rehabilitation exercise patients after total knee arthroplasty.

PMID:35776283 | DOI:10.1007/s10067-022-06266-0

Obes Surg. 2022 Jul 1. doi: 10.1007/s11695-022-06184-9. Online ahead of print.

ABSTRACT

BACKGROUND: Providing analgesia after bariatric surgery might be challenging due to a high prevalence of obstructive sleep apnea syndrome and the increased sensitivity to respiratory depression triggered by opioid overuse after surgery. Various combination methods with paracetamol, nonsteroidal anti-inflammatory drugs (NSAIDs), and other pain medications such as ketamine or gabapentin have been suggested for reduction of the opioid usage. Regional anesthetic techniques represent a valuable option as they improve patient comfort while reducing opioid-related side effects. In this study, we have evaluated the adjuvant benefits of these various techniques in reduction of the postoperative pain in bariatric surgery.

METHODS: After the approval of the IRB Ethics Committee, the records of the patients who had laparoscopic bariatric surgery between January 2019 and December 2021 were reviewed retrospectively.

RESULTS: Records of 120 patients who underwent laparoscopic bariatric surgery between January 2019 and December 2021 were reviewed. In total, 113 patients with full documentation were included in this study. Among these, 74 patients were administered regional analgesia. The main regional analgesia techniques were transversus abdominis plane and rectus sheath block. The pain scores of those receiving regional analgesia were statistically low. The opioid consumption after transversus abdominis plane and rectus sheath block was significantly lower than that of others. External oblique intercostal block alone provides a postoperative opioid consumption similar to those of transversus abdominis plane and rectus sheath block.

CONCLUSION: The use of fascial plane blocks in bariatric surgery significantly reduces opioid consumption. Transversus abdominis plane and rectus sheath block combination and external oblique intercostal block seem to be the most effective options.

CLINICAL TRIALS NUMBER: NCT05284695.

PMID:35776242 | DOI:10.1007/s11695-022-06184-9

J Genet Eng Biotechnol. 2022 Jul 1;20(1):91. doi: 10.1186/s43141-022-00360-1.

ABSTRACT

BACKGROUND: The utilization of bioluminescent bacteria in environmental monitoring of water contaminates considers being a vital and powerful approach. This study aimed to isolate, optimize, and apply luminescent bacteria for toxicity monitoring of various toxicants in wastewater.

RESULTS: On the basis of light intensity, strain Vibrio sp. 6HFE was initially selected, physiologically/morphologically characterized, and identified using the 16SrDNA gene. The luminescence production was further optimized by employing statistical approaches (Plackett-Burman design and central composite design). The maximum bioluminescence intensity recorded 1.53 × 10⁶ CPS using optimized medium containing (g/L), yeast extract (0.2g), CaCl₂ (4.0), MgSO₄ (0.1), and K₂HPO₄ (0.1) by 2.3-fold increase within 1h. The harnessing of Vibrio sp. 6HFE as a bioluminescent reporter for toxicity of organic solvents was examined using a bioluminescence inhibition assay. According to IC₅₀ results, the toxicity order of such pollutants was chloroform > isoamyl > acetic acid > formamide > ethyl acetate > acetonitrile > DMSO > acetone > methanol. However, among eight heavy metals tested, the bioluminescence was most sensitive to Ag⁺ and Hg⁺ and least sensitive to Co²⁺ and Ni²⁺. Additionally, the bioluminescence was inhibited by benzene, catechol, phenol, and penta-chlorophenol at 443.1, 500, 535.1, and 537.4 ppm.

CONCLUSION: Vibrio sp. 6HFE succeeded in pollution detection at four different environmental and wastewater samples revealing its efficiency in ecotoxicity monitoring.

PMID:35776216 | DOI:10.1186/s43141-022-00360-1

J Math Biol. 2022 Jul 1;85(1):3. doi: 10.1007/s00285-022-01768-6.

ABSTRACT

The linear noise approximation models the random fluctuations from the mean-field model of a chemical reaction that unfolds near the thermodynamic limit. Specifically, the fluctuations obey a linear Langevin equation up to order [Formula: see text], where [Formula: see text] is the size of the chemical system (usually the volume). In the presence of disparate timescales, the linear noise approximation admits a quasi-steady-state reduction referred to as the slow scale linear noise approximation (ssLNA). Curiously, the ssLNAs reported in the literature are slightly different. The differences in the reported ssLNAs lie at the mathematical heart of the derivation. In this work, we derive the ssLNA directly from geometric singular perturbation theory and explain the origin of the different ssLNAs in the literature. Moreover, we discuss the loss of normal hyperbolicity and we extend the ssLNA derived from geometric singular perturbation theory to a non-classical singularly perturbed problem. In so doing, we disprove a commonly-accepted qualifier for the validity of stochastic quasi-steady-state approximation of the Michaelis -Menten reaction mechanism.

PMID:35776210 | DOI:10.1007/s00285-022-01768-6

Clin Oral Investig. 2022 Jul 1. doi: 10.1007/s00784-022-04604-9. Online ahead of print.

ABSTRACT

OBJECTIVES: The assessment of whether different orthodontic pliers used in bracket debonding have different effects on pain and sensitivity experience.

MATERIALS AND METHODS: Thirty-three patients (17 females, 16 males) with metal brackets were included in the study. Compressed air and freshly melted ice water were applied to each tooth (6-6) in upper and lower arch before bracket debonding (T0), just after debonding (T1), and 1 week after debonding (T2). Bracket remover plier (BRP) and Weingart plier (WP) were used to debond brackets. A numeric rating scale (NRS) was used to assess sensitivity for each tooth at T0, T1, and T2. Tooth pain was assessed for each tooth using NRS during bracket debonding.

RESULTS: Statistically higher pain scores were found in teeth U4 (upper first premolar) (p = 0.017) and L6 (lower first molar) (p = 0.026) in Weingart plier group. No statistically significant difference was found during debonding in the other teeth between groups. Statistically high sensitivity score was found at T1 time point in tooth U3 (upper canine) by applying air stimulus in Weingart plier group (p = 0.024). There was no statistically significant difference between the sensitivity scores measured at T2 time point by applying air and cold stimuli between groups.

CONCLUSIONS: Although the debonding pain scores were statistically significant in two teeth and the sensitivity score in one tooth, there was no clinical significance between the two pliers in terms of pain and sensitivity.

CLINICAL RELEVANCE: Both debonding pliers gave clinically similar results in terms of pain and sensitivity.

PMID:35776201 | DOI:10.1007/s00784-022-04604-9

Clin Oral Investig. 2022 Jul 1. doi: 10.1007/s00784-022-04603-w. Online ahead of print.

ABSTRACT

OBJECTIVES: To investigate the knowledge, beliefs, and attitudes towards pediatric dental radiography and its associated factors among Turkish parents seeking dental care for their children.

MATERIALS AND METHODS: This cross-sectional study enrolled 483 parents of children aged 6-15 years. Data were collected using the questionnaire including socio-demographic characteristics, knowledge, beliefs, and attitudes based on The Health Belief Model and planned behavior theory. Descriptive, bivariate, and multivariate statistics were performed.

RESULTS: More than half of parents had correct knowledge on their rights to access health information, the radiation risk and exposure from dental X-rays, children’s vulnerability to radiation, and using lead apron. Most parents (70.4%) perceived dental X-rays as valuable. They had limited knowledge regarding the permanent damage and negative attitudes regarding its safety and goodness. There were positive correlations between knowledge-attitudes, knowledge-perceived benefits, and attitudes-perceived benefits (all p values < 0.01). Having a previous dental X-ray (p = 0.014) and not having parents’ information needs (p = 0.007) were associated with higher attitudes. Increasing education levels, having a educational need and higher perceived benefit were found to be predictors of higher parental knowledge.

CONCLUSION: Turkish parents had some knowledge and positive attitudes towards pediatric dental X-rays but not at optimum levels. Parents’ education levels, information needs, and beliefs were the most important factors affecting parental knowledge. Attitudes towards radiation safety and goodness should be improved.

CLINICAL RELEVANCE: These findings may provide useful insight to clinicians when developing theory-driven health education interventions on pediatric dental X-rays and identifiying the parents with low knowledge.

PMID:35776204 | DOI:10.1007/s00784-022-04603-w

Clin Oral Investig. 2022 Jul 1. doi: 10.1007/s00784-022-04582-y. Online ahead of print.

ABSTRACT

AIM: To describe and compare the pontic site development for fixed-dental prostheses (FDPs) with and without soft tissue grafting up to one-year post insertion of FDPs.

MATERIALS AND METHODS: A convenience sample of 24 patients participating in an ongoing RCT was provided with three-unit tooth-borne FDPs. Six patients received a subepithelial connective tissue graft (SCTG) at the pontic site, whereas 18 patients were treated without any soft tissue graft (CONTROL). Digital impressions were taken prior to tooth preparation, after tooth preparation, after insertion of the final FDP, and at the 1 year of follow-up. The obtained stereolithography files (STL) were superimposed and profilometric as well as linear changes of the soft tissue profile were assessed at the pontic regions. Profilometric outcomes included changes of the ridge contour, the alveolar ridge width, and the crown height of the pontic. Further outcomes assessed included: the papilla index, the pink esthetic score (PES), probing depth (PD), bleeding on probing (BOP), and plaque control record (PCR). Descriptive and nonparametric statistics were applied for all outcome measures.

RESULTS: The median profilometric contour between tooth preparation and 1 year after the insertion of the final FDP decreased by – 0.25 mm [Q1, Q3: – 0.36, 0.14] in the CONTROL group and increased by 0.61 mm [Q1, Q3: – 0.18, 1.06] in the SCTG group (intergroup p = 0.038). The alveolar ridge width between prior to tooth preparation and the one-year follow-up amounted to – 0.12 mm [Q1, Q3: – 0.74, 0.70] (= loss) in the CONTROL group and to 2.23 mm [Q1, Q3: 0.62, 3.86] (= gain) in the SCTG group (intergroup p = 0.032). At one year, the median crown height of the pontic tended to decrease by – 1.24 mm [Q1, Q3: – 2.05, – 1.05] in the SCTG group (intragroup p = 0.094) and by – 0.22 mm [Q1, Q3: – 0.58, 0.66] in the CONTROL group (intragroup p = 0.831), with significant differences between the groups (intergroup p = 0.022). The papilla index between prior to tooth preparation and one year of follow-up improved significantly in both groups (p < 0.05). Between FDP delivery and one year of follow-up, the PES values decreased significantly in the CONTROL group (intragroup p = 0.007), while in the SCTG group the change was not significant (intragroup p = 0.875). Clinical parameters (PD, BOP, and PCR) remained stable over time and did not differ between the groups at any time point (intergroup p > 0.05).

CONCLUSION: Within the limitations of the present study, soft tissue grafting tends to limit contour changes at pontic sites, thus maintaining the esthetic outcomes over time. The lack of soft tissue grafting results in stable clinical outcomes; however, it may lead to a decrease in aesthetic outcomes over time.

CLINICAL RELEVANCE: Autogenous soft tissue grafting seems to be a valid therapeutic option for the development of the pontic site to restore ridge defects prior to the delivery of fixed dental prostheses and to limit dimensional changes over time.

PMID:35776200 | DOI:10.1007/s00784-022-04582-y

J Clin Invest. 2022 Jul 1;132(13):e151666. doi: 10.1172/JCI151666.

ABSTRACT

Cancers avoid immune surveillance through an array of mechanisms, including perturbation of HLA class I antigen presentation. Merkel cell carcinoma (MCC) is an aggressive, HLA-I-low, neuroendocrine carcinoma of the skin often caused by the Merkel cell polyomavirus (MCPyV). Through the characterization of 11 newly generated MCC patient-derived cell lines, we identified transcriptional suppression of several class I antigen presentation genes. To systematically identify regulators of HLA-I loss in MCC, we performed parallel, genome-scale, gain- and loss-of-function screens in a patient-derived MCPyV-positive cell line and identified MYCL and the non-canonical Polycomb repressive complex 1.1 (PRC1.1) as HLA-I repressors. We observed physical interaction of MYCL with the MCPyV small T viral antigen, supporting a mechanism of virally mediated HLA-I suppression. We further identify the PRC1.1 component USP7 as a pharmacologic target to restore HLA-I expression in MCC.

PMID:35775490 | DOI:10.1172/JCI151666