Nevin Manimala

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2026 Feb;42(2):129-139.

ABSTRACT

Objective To investigate the effect of N-acetylglucosamine transferase 4 (GalNAc-T4) on the mitochondrial autophagy in H9c2 cell injury model induced by hypoxia/reoxygenation (H/R) in high glucose (HG) environment, and its regulatory mechanism on extracellular signal regulated kinase 1/2 (ERK1/2) signaling pathway. Methods The cell experiments were divided into five groups. Control group (Control): Cells were transfected with pc-NC cells. HG group: 50 mmol/L glucose was added to the culture medium, and cells were transfected with pc-NC. HG+H/R group: In addition to the HG treatment, cells were subjected to a low oxygen environment for 6 hours, followed by transfection with the pc-NC vector. Overexpression group (pc): Cells were transfected with the pc while undergoing HG+H/R treatment. ERK1/2 agonist epidermal growth factor (EGF) group: Cells were treated with HG+H/R and transfected with the pc, supplemented with 20 ng/mL EGF in the culture medium. The proliferation activity, apoptosis rate, mitochondrial membrane potential, the levels of reactive oxygen species (ROS), iron ions, and mitochondrial autophagy in each group of cells were detected. The expressions of GalNAc-T4, ERK1/2, phosphorylated ERK1/2 (p-ERK1/2), translocase of outer mitochondrial membrane 20 (TOM20), translocase of inner mitochondrial membrane 23 (TIM23), B cell lymphoma 2 (Bcl2), and Bcl2 associated X protein (BAX) were detected in cells. Results Overexpression of GalNAc-T4 significantly increased the proliferation activity, the mitochondrial membrane potential, and the expression of TOM20, TIM23, and Bcl2 in H9c2 cells in HG+H/R group, and decreased the cell apoptosis rate, the levels of ROS and iron ion. Meanwhile, it inhibited the mitochondrial autophagy and downregulated the expression of p-ERK1/2/ERK1/2 and BAX in cells. EGF partially reversed the protective effect of the overexpression of GalNAc-T4 on cardiomyocytes, and the differences were statistically significant. Conclusion Overexpression of GalNAc-T4 could significantly improve the mitochondrial dysfunction in H9c2 cells undergoing hypoxia/reoxygenation under high glucose conditions, inhibit oxidative stress and excessive mitochondrial autophagy, and reduce the apoptosis rate of cardiomyocytes. This may be related to its ability to block the activation of ERK1/2 signaling pathway.

PMID:41620966

Alcohol Clin Exp Res (Hoboken). 2026 Feb;50(2):e70225. doi: 10.1111/acer.70225.

ABSTRACT

BACKGROUND: Despite potentially harmful consequences, people routinely encounter alcohol adverts designed to increase consumption of alcohol in preference to safer alternatives. However, individuals differ in the degree to which such adverts elicit preferential alcohol consumption. This study builds upon and extends prior research by testing hypotheses concerning the impact of biased processing during advert viewing on subsequent alcohol craving and consumption.

METHOD: Sixty-eight undergraduate students viewed beer and soft drink adverts. In some viewing blocks, beer and soft drink adverts played simultaneously to assess participants’ attentional bias to beer adverts. In others, participants were asked to choose which type of advert to view, which assessed bias in volitional viewing choice. Participants subsequently rated their craving for beer before completing a taste test designed to yield a behavioral measure of preferential beer consumption.

RESULTS: Attentional bias to alcohol adverts predicted beer craving and preferential beer consumption. The association between attentional bias and preferential beer consumption was mediated by beer craving. An equivalent pattern of prediction was observed when using the viewing choice bias measure as the predictor. Importantly, when variation in either bias measure was statistically controlled for, the other continued to predict preferential beer consumption in a manner that was mediated by beer craving.

CONCLUSIONS: Two types of processing bias during advert viewing-viewing preference and attentional bias-were independently associated with subsequent preferential alcohol consumption. In both cases, these associations were accounted for by alcohol craving. The theoretical and applied implications of this are discussed.

PMID:41620957 | DOI:10.1111/acer.70225

JMIR Med Educ. 2026 Jan 30. doi: 10.2196/83779. Online ahead of print.

ABSTRACT

BACKGROUND: Structured educational programs for physicians in healthy longevity medicine (HLM) remain scarce. No published data yet document the impact of longevity-focused medical education on physicians. This study assesses the ramification of the HLM curriculum, certified by the American Council for Continuing Medical Education (ACCME), on physicians’ confidence in knowledge of HLM and clinical practice.

OBJECTIVE: This study aimed to evaluate the impact of accredited HLM education on physicians’ confidence in knowledge, and practice patterns, examining self-reported integration of HLM principles, professional attitudes, and career trajectories to determine the translational value of structured curricula in the emerging medical discipline.

METHODS: A cross-sectional online survey was conducted between March and April 2024 among physicians who had completed accredited HLM courses between January 2023 and February 2024. Invitations were sent globally to 590 eligible physicians; trainees and students were excluded. A total of 113 respondents completed the survey and were included in the analysis. The survey assessed self-reported changes in clinical implementation, confidence in HLM-related knowledge, and professional attitudes following course completion. Descriptive statistics and logistic regression analyses were performed (p < .05, 95% CIs).

RESULTS: Respondents represented 42 nationalities and were primarily trained in family medicine (27.4%) and internal medicine (15.93%). Overall, 96.5% reported increased confidence in HLM-related knowledge, with 47.8% indicating substantial improvement. More than half (55.8%) reported integrating HLM principles into routine patient assessments, and 80.5% reported more frequent discussions related to healthspan-focused care. Additionally, 23% initiated aging biomarker testing, 48.7% increased testing frequency, 52.2% reported a shift in their perspective on aging, and 73.5% anticipated full integration of HLM into mainstream medicine. Physicians practicing in specialized care demonstrated higher odds of reporting increased confidence in HLM knowledge compared with those in primary and preventive care (OR 4.46, 95% CI 1.55-12.79, p = .005).

CONCLUSIONS: Accredited education in HLM is associated with enhanced confidence in HLM knowledge, increased clinical engagement with HLM practices, and a shift in aging-related care paradigms. These findings underscore the critical role of structured HLM curricula in bridging the translational gap between geroscience and everyday medical practice. Nevertheless, systemic healthcare barriers impede widespread implementation, warranting policy-level strategies to support healthspan-oriented education and care models.

PMID:41620952 | DOI:10.2196/83779

Pak J Pharm Sci. 2026 Mar;39(3):878-886. doi: 10.36721/PJPS.2026.39.3.REG.12881.1.

ABSTRACT

BACKGROUND: Septic shock is a life-threatening complication of sepsis, often accompanied by cardiopulmonary dysfunction, which significantly increases the mortality of patients. Norepinephrine (NE) is a commonly used vasopressor in the treatment of septic shock, but single-drug therapy may not fully achieve cardiopulmonary protection. Antidiuretic hormone (ADH) has potential regulatory effects on hemodynamics and inflammation, but its combined efficacy with NE in cardiopulmonary protection for septic patients remains to be further verified.

OBJECTIVES: This study aims to investigate the effectiveness of ADH combined with NE in providing cardiopulmonary protection for septic patients.

METHODS: A total of 100 patients with septic shock admitted to our hospital from March 2022 to March 2023 were enrolled in this study. They were randomly divided into an observation group and a control group, with 50 patients in each group. The observation group was treated with ADH combined with NE, while the control group received NE monotherapy. Relevant indicators of the two groups were dynamically monitored and compared.

RESULTS: Statistical analysis showed that tumor necrosis factor-α (TNF-α), cardiac troponin I (cTnI), and brain natriuretic peptide (BNP) had significant differences in group effect, time effect, and interaction effect (P<0.05). Interleukin-1 (IL-1) and interleukin-6 (IL-6) only showed significant time effect (P<0.05), but no significant group effect or interaction effect (P>0.05). Arterial partial pressure of oxygen (PaO₂) and PaO2/fraction of inspired oxygen (P/F ratio) exhibited significant time effect and group effect (P<0.05), but no significant interaction effect (P>0.05). After treatment (T3), compared with the control group, the observation group had significantly lower levels of TNF-α, IL-1, IL-6, cTnI, BNP, and creatine kinase isoenzyme MB (CK-MB) (P<0.05), and significantly higher PaO₂ and P/F ratio (P<0.05).

CONCLUSION: ADH combined with NE can effectively improve the inflammatory response, cardiac and pulmonary function indexes of septic patients, and exert significant cardiopulmonary protection effects. It is a viable and effective therapeutic strategy for septic patients.

PMID:41620916 | DOI:10.36721/PJPS.2026.39.3.REG.12881.1

Pak J Pharm Sci. 2026 Mar;39(3):597-603. doi: 10.36721/PJPS.2026.39.3.REG.14269.1.

ABSTRACT

BACKGROUND: Diabetic macular edema (DME) is a predominant cause of visual impairment among patients. The concurrent administration of anti-vascular endothelial growth factor (VEGF) agents and corticosteroids may yield synergistic therapeutic benefits.

OBJECTIVE: In this study, we evaluated the efficacy and safety of aflibercept (AFL) in combination with dexamethasone (DEX) intravitreal implant for the treatment of DME.

METHODS: A total of 80 patients with DME admitted to a hospital from June 2022 to June 2024 were enrolled. 42 patients in the AFL group received monotherapy, and 38 patients in the AFL+DEX group received combination therapy. The best corrected visual acuity (BCVA), central macular thickness (CMT), intraocular pressure (IOP) and aqueous humor inflammatory factors (IL-1β, MCP-1, IL-6, VEGF) were evaluated monthly. Efficacy was graded according to criteria at 4 months after treatment, and adverse events were recorded.

RESULTS: The findings revealed no statistically significant difference in the overall clinical efficacy rate between the AFL+DEX group and the AFL group (P>0.05). However, the AFL+DEX group demonstrated superior best-corrected visual acuity (BCVA) at 1, 3 and 6 months post-treatment, alongwith a significant reduction in central macular thickness (CMT) compared to the AFL group (P<0.05). Furthermore, aqueous humor analysis indicated markedly lower levels of inflammatory cytokines in the AFL+DEX group following treatment (P<0.05). In terms of safety profiles, the AFL+DEX group required fewer intravitreal injections (P<0.05).

CONCLUSION: These findings underscore the potential of AFL combined with DEX intravitreal implant to enhance visual outcomes and modulate intraocular inflammation in DME patients, highlighting its substantial clinical utility.

PMID:41620889 | DOI:10.36721/PJPS.2026.39.3.REG.14269.1

Wiad Lek. 2025;78(12):2830-2836. doi: 10.36740/WLek/214048.

ABSTRACT

OBJECTIVE: Aim: To identify and analyze the factors that mandate the technologization of iatrogenic crime investigation process and to propose actionable strategies for integrating modern technologies and algorithms into investigative practices for this class of criminal proceedings.

PATIENTS AND METHODS: Materials and Methods: This research is based on an analysis of official WHO documents, 31 relevant judgments from the European Court of Human Rights (ECHR) concerning adverse medical outcomes, 19 verdicts and decisions from Ukrainian courts on medical malpractice, and statistical data from the Prosecutor General’s Office of Ukraine for the period 2016-2021. The primary analysis is supplemented by a review of recent scientific articles from leading academic databases.

CONCLUSION: Conclusions: It has been proven that although patient harm resulting from the provision of low-quality medical care is very widespread worldwide and often becomes the subject of criminal investigation, Ukraine demonstrates a very low level of investigation and detection of iatrogenic crimes. It is proposed that these shortcomings be corrected through technologization, which includes the algorithmization of investigative actions and the application of the latest technologies.

PMID:41620885 | DOI:10.36740/WLek/214048

Wiad Lek. 2025;78(12):2742-2750. doi: 10.36740/WLek/214419.

ABSTRACT

OBJECTIVE: Aim: Identifying of key criteria that influence the process of dispensing medicines by pharmacists by electronic prescriptions, and establish its’ relationship with the frequency of problems while paying off prescriptions.

PATIENTS AND METHODS: Materials and Methods: In our work we used the survey method involving questionnaires. To screen the obtained results, we used the method of intelligent data analysis depending on building the associative rules. The survey subjects were pharmaceutical clerks employed by pharmacies in the Western, Central, Eastern, Northern and Southern regions of Ukraine, who directly sold MPs to the population. Respondents included individuals of different sexes, ages, and professional experience. The questionnaire contained several blocks that were dedicated to the analysis and identification of the main difficulties that arise when dispensing drugs by e-prescriptions and the criteria that influence the choice of a drug for dispensing by trade name when dispensing by e-prescriptions. A total of 2233 specialists were surveyed.

RESULTS: Results: We analyzed the criteria that had influenced a choice of trade name of prescription medicines filled for patients by a pharmacist under e-prescription in the form of an international non-proprietary name.

CONCLUSION: Conclusions: We have determined the criteria for selecting medicinal products by pharmaceutical clerks while filling e-prescriptions, which can be used in the practical activities of pharmaceutical workers as an integral part of the process of optimizing pharmacy inventories. The most important criteria have been found the amount of co-payment for medicine, the country of manufacture/medicines manufacturer, and the history of medicine administration by the patient. The criteria of the presence or absence of evidence-based data on the efficacy of a particular medicine and the medicine`s belonging to generics or original medicines have been found the least important. The inability of a pharmaceutical clerk/pharmacy worker to select a medicine based on one or more of the above selection criteria is considered one of the reasons for failure to dispense up to 20% of medicinal products under e-prescriptions.

PMID:41620874 | DOI:10.36740/WLek/214419

Wiad Lek. 2025;78(12):2707-2716. doi: 10.36740/WLek/213629.

ABSTRACT

OBJECTIVE: Aim: The relationship between diabetes mellitus (DM) and lifestyle quality become important in diabetes research in last year. The present study aims to study the influence of metformin response in sleep in diabetes mellitus patients type 2.

PATIENTS AND METHODS: Materials and Methods: A cross sectional study was designed to achieve study goal, glycemic parameters included fasting blood glucose (FBG), glycated protein (HbA1c%), insulin (IN), insulin resistance (HOMA-IR) and insulin sensitivity (IS). PCR sequencing was used to detect SLC47A2 intronic variants and its related with glycemic control and sleep status.

RESULTS: Results: Among the study population, about 26.3% achieved well glycemic control, 30% were moderately controlled, and 43.8% were poorly controlled. Sleep quality assessment showed that the majority of participants in all glycemic groups experienced intermediate sleep. The prevalence of insomnia increased with worsening glycemic control, from 4.8% in the well-controlled group to 17.1% in poorly controlled participants, in non- statistically significant (p = 0.722). Biochemical parameters confirmed significant differences in fasting blood glucose and HbA1c across the three glycemic categories (p < 0.001), insulin, HOMA-IR, and insulin sensitivity did not differ significantly. Multiple regression analyses indicated that none of the biochemical predictors significantly explained sleep in any group (p > 0.05), in poorly controlled patients, non-significant opposing trends were observed for insulin and insulin resistance, sociodemographic factors included supplement use, education level, and employment were associated with better sleep among poorly controlled patients. Genetic analysis of two intronic variants in the SLC47A2 gene (g.19716681G>C and rs1597652185) revealed no significant associations with glycemic control or sleep, though both showed similar distribution patterns across groups. Statistical analysis didn’t find significant association between either variant and glycemic or sleep status (p > 0.05).

CONCLUSION: Conclusions: Poor glycemic control was common and associated with higher insomnia prevalence. While demographic and clinical factors showed no clear links with glycemic control or sleep, supplement use emerged as a protective factor. FBG and HbA1c strongly differentiated control groups, but other biomarkers and SLC47A2 variants were not predictive. Findings suggest that combining metabolic management with supportive measures like supplementation may improve sleep and outcomes in type 2 diabetes..

PMID:41620870 | DOI:10.36740/WLek/213629

Wiad Lek. 2025;78(12):2677-2684. doi: 10.36740/WLek/215808.

ABSTRACT

OBJECTIVE: Aim: To assess hormonal status and vitamin D metabolic features in women of reproductive age presenting with abnormal uterine bleeding and associated extragenital conditions.

PATIENTS AND METHODS: Materials and Methods: The study was conducted at the clinical base of the Department of Obstetrics, Gynecology, Pediatric Gynecology, and Medical Genetics of Kharkiv National Medical University (KhNMU). A total of 110 women of reproductive age were examined and divided into two clinical groups. The first group (main group, MG) included 80 patients diagnosed with abnormal uterine bleeding (AUB) in combination with extragenital cconditions. The second group (control group, CG) consisted of 30 somatically healthy women who presented for routine preventive examinations. Hormonal status was assessed by measuring serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone (PRG), estradiol (E2), and vitamin D using enzyme-linked immunosorbent assay (ELISA) methods. Statistical analysis was performed using the general-purpose software package Statistica 6.0.

RESULTS: Results: The average age of the participants was 31.5 ± 13.5 years. Among women in the main group, the most frequently observed extragenital conditions were diseases of the endocrine, circulatory, and digestive systems. Analysis of serum hormone levels revealed a decrease in FSH (1.84-fold), LH (1.64-fold), and PRG (2.6-fold) (KWT, p < 0.01). Conversely, E2 levels were elevated by 3.52-fold in the MG (KWT, p < 0.01). Serum vitamin D levels in the MG were reduced by 2.25-fold compared to the CG (KWT, p < 0.01). A statistically significant (p < 0.05) positive correlation was found between serum E2 and vitamin D levels (r = 0.61).

CONCLUSION: Conclusions: The study revealed distinct features of the hormonal profile and vitamin D metabolism in women of reproductive age with abnormal uterine bleeding and concomitant extragenital conditions. Specifically, this patient population demonstrated decreased serum levels of LH, FSH, PRG, and vitamin D, accompanied by hyperestrogenemia. A statistically significant correlation was observed between serum E2 and vitamin D levels.

PMID:41620867 | DOI:10.36740/WLek/215808