Nevin Manimala

PLoS One. 2024 Jan 9;19(1):e0292669. doi: 10.1371/journal.pone.0292669. eCollection 2024.

ABSTRACT

Although CO2 field-flooding was first used during cardiac surgery more than 60 years ago, its efficacy is still disputed. The invisible nature of the gas and the difficulty in determining the “safe” quantity to protect the patient are two of the main obstacles to overcome for its validation. Moreover, CO2 concentration in the chest cavity is highly sensitive to procedural aspects, such suction and hand movements. Based on our review of the existing literature, we identified four major factors that influence the intra-cavity CO2 concentration during open-heart surgery: type of delivery device (diffuser), delivery CO2 flow rate, diffuser position around the wound cavity, and its orientation inside the cavity. In this initial study, only steady state conditions were considered to establish a basic understanding on the effect of the four above-mentioned factors. Transient factors, such as suction or hand movements, will be reported separately.

PMID:38194426 | DOI:10.1371/journal.pone.0292669

Pak J Biol Sci. 2023 Oct;26(11):576-585. doi: 10.3923/pjbs.2023.576.585.

ABSTRACT

<b>Background and Objective:</b> Indonesia boasts a variety of delicious tropical fruits, including pomelo, mainly grown in Pangkep Regency, South Sulawesi Province. However, in this region, some challenges hinder such as inadequate care, aging trees and limited seed supply hinder productivity in this region. <i>In vitro</i> culture methods present a solution by rapidly producing high quality, disease-free pomelo seeds. This study aims to determine the optimal concentration of the BAP added to the culture medium to induce shoots from pomelo seeds. <b>Materials and Methods:</b> The seeds were planted on MS media with the addition of BAP hormone (0.5, 1, 1.5, 2 and 2.5 ppm) and 0 ppm as the control. The experimental units were arranged in a CRD and analyzed using SPSS 20.0 software, employing the Shapiro-Wilk normality test and Levene’s Statistic for homogeneity. If the data met the normality and homogeneity assumptions, ANOVA was applied, followed by the DMRT for a parametric test. Otherwise, a non-parametric test namely the Kruskal-Wallis was conducted and differences were further analyzed using the Mann-Whitney test at a 5% significance level. <b>Results:</b> The application of the BAP accelerated shoot emergence, with the most rapid development occurring on the 10th day after planting (DAP), at a BAP concentration of 2.5 ppm for red pomelo. For white pomelo and sweet pomelo, shoots appeared on the 19th and 20th days, respectively at a 2 ppm BAP. Interestingly, root development was fastest between the 4th and 6th DAP in 0 ppm BAP (control). <b>Conclusion:</b> The addition of the BAP at a concentration of 1.5 ppm in the culture medium promotes faster shoot emergence and has a significant impact on the number of shoots in red pomelo.

PMID:38193372 | DOI:10.3923/pjbs.2023.576.585

Pak J Biol Sci. 2023 Oct;26(11):549-556. doi: 10.3923/pjbs.2023.549.556.

ABSTRACT

<b>Background and Objective:</b> Fixed oils used in traditional therapies also called volatile oils are generally aromatic oils obtained by the steam or hydrodistillation of plants. Different parts of plants have been used to obtain fixed oils. This study estimates the chemical constituents and <i>in vitro</i> antibacterial activity of fixed oils extracted by petroleum ether from the leaves, roots, stems and fruit part of <i>Bridelia stipularis</i> (L.). <b>Materials and Methods:</b> The natural fatty acids were extracted from different parts of <i>B. stipularis</i> by using petroleum ether. The fixed oils were studied by gas chromatography-mass spectrophotometry. The antibacterial test was carried out by the agar disc diffusion method. A Student’s t-test was computed for the statistical significance of the results. <b>Results:</b> It showed 10 compounds from the leaf and 5 compounds from the stem. In both cases, the major components were methyl decanoate 93.56 and 74.98%, respectively. From the root parts, 6 compounds were identified in which the major compound was methyl linolelaidate (36.86%). Two compounds were identified from the fruit part and the major portion was methyl pentadecanoate (98.20%). The <i>in vitro</i> antibacterial potentials of the oils were tested against four pathogenic bacteria. Among the four fixed oils, the stem, leaf and root showed the strongest activity against <i>E. coli</i> (30, 21 and 15 mm). On the other hand, fruit fixed oil showed the highest zone of inhibition against <i>Bacillus cereus</i> (25 mm). <b>Conclusion:</b> The fixed oils of <i>B. stipularis</i> plant have the potential to be applied as an antibacterial agent, which can be selected for further analysis and can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals that address unmet therapeutic needs.

PMID:38193369 | DOI:10.3923/pjbs.2023.549.556

Magn Reson Med. 2024 Jan 9. doi: 10.1002/mrm.29994. Online ahead of print.

ABSTRACT

PURPOSE: The determination of blood-brain barrier (BBB) integrity and partial pressure of oxygen (pO₂ ) in the brain is of substantial interest in several neurological applications. This study aimed to assess the feasibility of using trityl OX071-based pulse electron paramagnetic resonance imaging (pEPRI) to provide a quantitative estimate of BBB integrity and pO₂ maps in mouse brains as a function of neuroinflammatory disease progression.

METHODS: Five Connexin-32 (Cx32)-knockout (KO) mice were injected with lipopolysaccharide to induce neuroinflammation for imaging. Three wild-type mice were also used to optimize the imaging procedure and as control animals. An additional seven Cx32-KO mice were used to establish the BBB leakage of trityl using the colorimetric assay. All pEPRI experiments were performed using a preclinical instrument, JIVA-25 (25 mT/720 MHz), at times t = 0, 4, and 6 h following lipopolysaccharide injection. Two pEPRI imaging techniques were used: (a) single-point imaging for obtaining spatial maps to outline the brain and calculate BBB leakage using the signal amplitude, and (b) inversion-recovery electron spin echo for obtaining pO₂ maps.

RESULTS: A statistically significant change in BBB leakage was found using pEPRI with the progression of inflammation in Cx32 KO animals. However, the change in pO₂ values with the progression of inflammation for these animals was not statistically significant.

CONCLUSIONS: For the first time, we show the ability of pEPRI to provide pO₂ maps in mouse brains noninvasively, along with a quantitative assessment of BBB leakage. We expect this study to open new queries from the field to explore the pathology of many neurological diseases and provide a path to new treatments.

PMID:38193348 | DOI:10.1002/mrm.29994

J Am Heart Assoc. 2024 Jan 9:e029827. doi: 10.1161/JAHA.123.029827. Online ahead of print.

ABSTRACT

BACKGROUND: Soluble suppression of tumorigenicity-2 (sST2) predicts mortality in patients with heart failure. The predictive value of sST2 in patients with a left ventricular assist device remains unknown. Therefore, we studied the relationship between sST2 and outcome after left ventricular assist device implantation.

METHODS AND RESULTS: sST2 levels of patients with a left ventricular assist device implanted between January 2015 and December 2022 were included in this observational study. The median follow-up was 25 months, during which 1573 postoperative sST2 levels were measured in 199 patients, with a median of 29 ng/mL. Survival of patients with normal and elevated preoperative levels was compared using Kaplan-Meier analysis, which did not differ significantly (P=0.22) between both groups. The relationship between postoperative sST2, survival, and right heart failure was evaluated using a joint model, which showed a significant relationship between the absolute sST2 level and mortality, with a hazard ratio (HR) of 1.20 (95% CI, 1.10-1.130; P<0.01) and an HR of 1.22 (95% CI, 1.07-1.39; P=0.01) for right heart failure, both per 10-unit sST2 increase. The sST2 instantaneous change was not predictive for survival or right heart failure (P=0.99 and P=0.94, respectively). Multivariate joint model analysis showed a significant relationship between sST2 with mortality adjusted for NT-proBNP (N-terminal pro-B-type natriuretic peptide), with an HR of 1.19 (95% CI, 1.00-1.42; P=0.05), whereas the HR of right heart failure was not significant (1.22 [95% CI, 0.94-1.59]; P=0.14), both per 10-unit sST2 increase.

CONCLUSIONS: Time-dependent postoperative sST2 predicts all-cause mortality after left ventricular assist device implantation after adjustment for NT-proBNP. Future research is warranted into possible target interventions and the optimal monitoring frequency.

PMID:38193339 | DOI:10.1161/JAHA.123.029827

J Korean Med Sci. 2024 Jan 8;39(1):e25. doi: 10.3346/jkms.2024.39.e25.

ABSTRACT

BACKGROUND: Shift work, including night shift work, during pregnancy has been associated with adverse birth outcomes such as small for gestational age (SGA) infants and preterm births. This study, conducted in South Korea using the Korean CHildren’s ENvironmental health Study (Ko-CHENS) cohort, aimed to investigate the association between shift work and night shift status during pregnancy and adverse birth outcomes.

METHODS: The Korean Ko-CHENS is a nationwide prospective birth cohort study of children’s environmental diseases, conducted by the Ministry of Environment and the National Institute of Environmental Research. This study included pregnant women recruited from 2015 to 2020 for Ko-CHENS Core Cohorts, and 4,944 out of a total of 5,213 pregnant women were selected as final subjects. A logistic regression model was used to identify the risk factors affecting SGA births, preterm births, and low-birth-weight infants, and the odds ratio (OR) was adjusted. This was confirmed by calculating ORs. Maternal age, infant sex, maternal educational status, body mass index, smoking status, alcohol consumption status, parity, gestational diabetes mellitus, preeclampsia, and abortion history were used as adjusted variables.

RESULTS: No statistically significant differences were observed in the birth outcomes or maternal working patterns. There were no significant differences in the adjusted odds ratios (aORs) of SGA and preterm births between the non-worker, day worker, and shift worker. However, there was a significant difference in the aORs of SGA between non-workers and night shift workers. (aORs [95% confidence interval], 2.643 [1.193-5.859]).

CONCLUSION: Working during pregnancy did not increase the risk of SGA or preterm birth, and night shift work did not increase the risk of preterm birth. However, night-shift work increases the risk of SGA.

PMID:38193332 | DOI:10.3346/jkms.2024.39.e25

J Korean Med Sci. 2024 Jan 8;39(1):e9. doi: 10.3346/jkms.2024.39.e9.

ABSTRACT

BACKGROUND: Turner syndrome (TS) is a common chromosomal abnormality, which is caused by loss of all or part of one X chromosome. Hormone replacement therapy in TS is important in terms of puberty, growth and prevention of osteoporosis however, such a study has never been conducted in Korea. Therefore, the purpose of our study was to determine relationship between the starting age, duration of estrogen replacement therapy (ERT) in TS and develop a hormone replacement protocol suitable for the situation in Korea.

METHODS: This is retrospective study analyzed the medical records in TS patients treated at the Severance hospital, Yonsei University College of Medicine, Seoul, Korea from 1997 to 2019. Total of 188 subjects who had received a bone density test at least once were included in the study. Korean National Health and Nutrition Examination Survey (KNHANES) was used for achieving bone mineral density (BMD) of normal control group. Student’s t-test, Mann-Whitney U test, ANOVA and correlation analysis were performed using SPSS 18.0.

RESULTS: Each BMD measurement was significantly lower in women with TS than in healthy Korean women. Early start and longer duration of ERT is associated with higher lumbar spine BMD but not femur neck BMD. Femur neck BMD, but not lumbar spine BMD was significantly higher in women with mosaicism than 45XO group.

CONCLUSION: Early onset and appropriate duration of hormone replacement therapy is important for increasing bone mineral density in patients with Turner syndrome. Also, ERT affects differently to TS patients according to mosaicism.

PMID:38193328 | DOI:10.3346/jkms.2024.39.e9

Int J Gynaecol Obstet. 2024 Jan 9. doi: 10.1002/ijgo.15350. Online ahead of print.

ABSTRACT

OBJECTIVE: To consider the classical use of “pH < 7.0 and/or a base deficiency ≥12 mmol/L” as markers of the risk of neonatal hypoxic-ischemic encephalopathy (HIE), recalling various criticisms of the use of these markers in favor of that of neonatal eucapnic pH, which appears to be a better marker of this risk.

METHODS: Fifty-five cases of acidemia with pH < 7.00 were collected from a cohort from the Nice University Hospital with eight cases of HIE. We compared the receiver operating characteristics curves established from the positive likelihood ratio (+LR) for each case of: umbilical cord artery pH (pHa), neonatal eucapnic pH (pH euc-n) in isolation (not matched to pHa), and matched pHa to its own pH euc-n.

RESULTS: The areas under the curve (AUC) are identical for pHa and pH euc-n, but AUC for the matched pair pHa-pH euc-n appears superior but non-significant because of the small number in our cohort. However, using the bootstrap method, the partial AUC for a sensitivity greater than 75% indicates the significant superiority (P < 0.01) of the matched pair pHa-pH euc-n approach.

CONCLUSION: The originality of this study lies in the use of two methodologic approaches: (1) standardized partial analysis of the AUCs of the pHa curve and that of pHa matched to its own pH euc-n, and (2) bootstrap statistical technique, that allowed us to conclude (P < 0.01) that the combined use of pH measured at the cord coupled with its eucapnic correction is better for diagnosing metabolic acidosis and best predicting the risk of HIE.

PMID:38193307 | DOI:10.1002/ijgo.15350

J Dent Educ. 2024 Jan 9. doi: 10.1002/jdd.13447. Online ahead of print.

ABSTRACT

OBJECTIVES: This study examined results of a summative objective structured clinical examination (OSCE) at the end of preclinical periodontal education to identify deficient areas for dental students in patient care and to explore factors affecting the probability of passing the OSCE.

METHODS: The summative OSCE was administered to two consecutive cohorts, Classes A (class of 2024; n₁ = 134) and B (class of 2025; n₂ = 129). The questions for each station in the OSCEs were available to both classes 1 week before the OSCEs. Descriptive statistics were used to identify deficient areas. The multiple logistic regression model was built to predict the probability of passing the OSCE based on the cohort, gender, and the practical and written examination scores.

RESULTS: Fifty-one (38%) students in Class A and 66 (51%) students in Class B completed the OSCE by passing all stations. Students undergoing remediation showed deficiencies in demonstrating how to detect tooth mobility, performing periodontal probing, drawing the healthy positive bony architecture and the mucogingival junction, and using a universal and a Gracey 13/14 curette. The probability of passing the OSCE was significantly correlated with Class B (p = 0.035) and the practical examination score (p = 0.03) while not associated with gender (p = 0.53) and the written examination score (p = 0.11).

CONCLUSION: Students showed deficiencies in assessment skills at the end of preclinical education. The study findings suggest that the implementation of the OSCE at the conclusion of preclinical education would be beneficial since the written examination score might not accurately reflect student readiness for clinical patient care.

PMID:38193239 | DOI:10.1002/jdd.13447

Arch Ital Urol Androl. 2023 Dec 29;95(4):11691. doi: 10.4081/aiua.2023.11691.

ABSTRACT

BACKGROUND: Renal transplantation stands as the sole remedy for individuals afflicted with end-stage renal diseases, and safeguarding them from transplant rejection represents a vital, life-preserving endeavor posttransplantation. In this context, the impact of cytokines, notably IL-27, assumes a critical role in managing immune responses aimed at countering rejection. Consequently, this investigation endeavors to explore the precise function of IL-27 and its associated cytokines in the context of kidney transplant rejection.

METHODS: The study involved the acquisition of blood samples from a cohort of participants, consisting of 61 individuals who had undergone kidney transplantation (comprising 32 nonrejected patients and 29 rejected patients), and 33 healthy controls. The expression levels of specific genes were examined using SYBR Green Real-time PCR. Additionally, the evaluation encompassed the estimation of the ROC curve, the assessment of the relationship between certain blood factors, and the construction of protein-protein interaction networks for the genes under investigation.

RESULTS: Significant statistical differences in gene expression levels were observed between the rejected group and healthy controls, encompassing all the genes examined, except for TLR3 and TLR4 genes. Moreover, the analysis of the Area Under the Curve (AUC) revealed that IL-27, IL-27R, TNF-α, and TLR4 exhibited greater significance in discriminating between the two patient groups. These findings highlight the potential importance of IL-27, IL-27R, TNF-α, and TLR4 as key factors for distinguishing between individuals in the rejected group and those in the healthy control group.

CONCLUSIONS: In the context of kidney rejections occurring within the specific timeframe of 2 weeks to 2 months post-transplantation, it is crucial to emphasize the significance of cytokines mRNA level, including IL-27, IL-27R, TNF-α, and TLR4, in elucidating and discerning the diverse immune system responses. The comprehensive examination of these cytokines’ mRNA level assumes considerable importance in understanding the intricate mechanisms underlying kidney rejection processes during this critical period.

PMID:38193229 | DOI:10.4081/aiua.2023.11691