Nevin Manimala

Zhonghua Jie He He Hu Xi Za Zhi. 2022 Sep 12;45(9):888-893. doi: 10.3760/cma.j.cn112147-20220127-00078.

ABSTRACT

Objective: To investigate the development of tuberculosis screening-related tests in general public hospitals(GPHs) of different levels in Hunan Province and the”awareness and practice of screening tuberculosis in diabetic patients”by doctors directly involved in diabetes diagnosis and treatment in the hospitals, aiming to provide reference for the formulation of the tuberculosis-diabetes joint prevention and control activity plan based on our national conditions. Methods: Stratified sampling was used to select 43 GPHs at three different levels in Hunan province: 14 tertiary GPHs, 13 secondary GPHs, and 16 primary GPHs. 284 endocrinologists working in enrolled hospitals were invited to participate in the on-site questionnaire-survey and 277 qualified. The study used SPSS 22.0 statistical software to analyze the data. The prevalence rate of tuberculosis screening test among hospitals at all levels was compared by chi-square test, and logistic regression was used to analyze the related factors affecting doctors’ screening awareness. P≤0.05 was considered statistically significant. Results: The allocation of digital X-ray cameras, tuberculin skin tests, sputum acid-fast bacillus smears, sputum cultures for Mycobacterium tuberculosis, and interferon-gamma release assays in the 43 GPHs were 90.7% (39/43), 72.1% (31/43), 55.8% (24/43), 34.9% (15/43), 27.9% (12/43) with significant differences between the different hospital levels(P<0.05). 173 endocrinologists considered it necessary to proactively initiate tuberculosis screening for patients at first diagnosis. When admitting patients, 197 endocrinologists chose tuberculosis screening only for diabetes mellitus patients with suspected tuberculosis symptoms. The most possible reasons why diabetes mellitus patients wouldn’t undergo tuberculosis screening were”patients refused(76.5%, 212/277)”,”patients didn’t complain of the symptom(46.9%, 130/277)”, and”tuberculosis screening-related tests haven’t been conducted in the hospital(35.7%, 99/277)”. Conclusions: Although endocrinologists displayed some tuberculosis-related knowledge and awareness of the need for proactive tuberculosis screening, the actual screening rate in the clinical setting was low. This may be related to multiple factors, including those of patients, doctors, and medical institutions.

PMID:36097926 | DOI:10.3760/cma.j.cn112147-20220127-00078

Vet Med Sci. 2022 Sep 12. doi: 10.1002/vms3.952. Online ahead of print.

ABSTRACT

BACKGROUND: Nowadays ingestion of indigestible foreign bodies poses serious economic loss to domestic ruminants, especially in a developing country.

OBJECTIVES: The study aims to determine the prevalence of indigestible foreign bodies in the forestomach of domestic ruminants slaughtered at Bishoftu Elfora Export Abattoir and its associated potential risk factors and identify the location and type of these indigestible foreign bodies.

STUDY ANIMALS: On 384 domestic ruminants (171 goats, 125 sheep and 88 cattle) presented for slaughter from different zones of the region.

METHODS: Cross-sectional study design was conducted from May 2021 to September 2021. Each animal was subjected to ante-mortem and post-mortem inspection by providing a unique identification number. For post-mortem examination, the forestomach was carefully removed from the abdominal cavity. For the positives, the location was properly recorded, washed, identified and photographed.

RESULTS: The overall prevalence of indigestible foreign bodies in domestic ruminants was 30.73% (118/384). Of these, 32.75% (56/171), 28% (35/125) and 30.68% (27/88) were recorded in goats, sheep and cattle, respectively, without statistically significant difference (p > 0.05). Aged domestic ruminants 39.3% (33/84) had poor body conditions 38.98% (23/59), originated from the Borena zone 42.11% (16/38) and were relatively recorded with higher prevalence. From the forestomach compartments, the indigestible foreign bodies were majorly found in rumen 86.43% (102/118) and reticulum 13.56% (16/118) with a statistical difference (p < 0.05).

CONCLUSION AND CLINICAL IMPORTANCE: Ingestion of indigestible foreign bodies mainly during the dry season due to shortage of forage, less biodegradable and negligent waste disposal like plastic bags attribute environmental and animal health problems and pose loss to the national economy.

PMID:36097138 | DOI:10.1002/vms3.952

Mol Biol Rep. 2022 Sep 12. doi: 10.1007/s11033-022-07761-y. Online ahead of print.

ABSTRACT

BACKGROUND: In cancer research, one of the most significant findings was to characterize the DNA repair deficiency as carcinogenic. Amongst the various repair mechanisms, mismatch repair (MMR) and direct reversal of DNA damage systems are designated as multilevel safeguards in the human genome. Defects in these elevate the rate of mutations and results in dire consequences like cancer. Of the several molecular signatures in human genome, tandem repeats (TRs) appear at various frequencies in the exonic, intronic, and regulatory regions of the DNA. Hypervariability among these repeats in the coding and non-coding regions of the genes is well characterized for solid tumours, but its significance in haematologic malignancies remains to be explored. The purpose of our study was to elucidate the role of nucleotide repeat instability in the coding and non-coding regions of 10 different repair genes in myeloid and lymphoid cell lines compared to the control samples.

METHODS AND RESULTS: We selected MMR deficient extensively studied microsatellite instable colorectal cancer (HCT116), and MMR proficient breast cancer (MCF-7) cells along with underemphasized haematologic cancer cell lines to decipher the hypermutability of tandem repeats. A statistically significant TR variation was observed for MSH2 and MSH6 genes in 4 and 3 of the 6 cell lines respectively. KG1 (AML) and Daudi (Burkitt’s lymphoma) were found to have compromised DNA repair competency with highly unstable nucleotide repeats.

CONCLUSION: Taken together, the results suggest that mutable TRs in intronic and non-intronic regions of repair genes in blood cancer might have a tumorigenic role. Since this is a pilot study on cell lines, high throughput research in large cohorts can be undertaken to reveal novel diagnostic markers for unexplained blood cancer patients with normal karyotypes or otherwise with karyotypic defects.

PMID:36097110 | DOI:10.1007/s11033-022-07761-y

Neurosurg Rev. 2022 Sep 12. doi: 10.1007/s10143-022-01858-5. Online ahead of print.

ABSTRACT

Postoperative seizures are a frequently occurring yet not well-understood complication in patients undergoing surgical treatment of chronic subdural haematomas (cSDHs). Therefore, we investigated surgical and non-surgical risk factors that are commonly considered causal in provoking epileptic seizures, paying special attention to the intracranial course of the subdural drain (SDD) and the configuration of the haematoma. Data of patients with a cSDH, that were treated at our neurosurgical department between 2008 and 2014 were analysed. Patients suffering from severe pre-existing conditions and those who have been treated conservatively were excluded. Epidemiologic data as well as relevant clinical data were collected. Pre- and postoperative CT scans were analysed regarding morpho- and volumetric parameters. In order to objectify the influence of the SDD, its intracranial course and localisation (entering angle as well as the angle between drain and brain surface) were measured. For statistical analysis, univariate and multiple logistic regression models as well as Fisher’s exact test were used. Two hundred eleven consecutive patients have been included. Mean age was 75.6 years, and 69% were male. Nineteen (9%) patients suffered from postsurgical seizures. Membranes within the haematoma were present in 81.5%. Pre- to postoperative haematoma reduction was significant (mean of difference – 12.76 mm/ – 9.47 mm in coronal/axial CT planes, p = 0.001/ < 0.001). In 77.9%, SDD showed cortical contact with eloquent regions and had an unfavourable course in 30 cases (14.2%). Surgical complications consisted of cortical bleeding in 2.5%, fresh subdural haematoma in 33.5% and wound infections in 1.4% of patients. Neither in univariate nor in multiple regression analyses any of the following independent variates was significantly correlated with postsurgical seizures: pre-existing epilepsy, alcohol abuse, right-sided haematomas, localization and thickness of haematoma, presence of septations, SDD-localization and to-brain angle, subdural air, and electrolyte levels. Instead, in multiple regression analyses, we found the risk of postsurgical seizures to be significantly correlated and increased with left-sided cSDH treated via craniotomy (p = 0.03) and an unfavourable course of the SDD in left-sided cSDH (p = 0.033). Burr hole trepanation should be preferred over craniotomy and care must be taken when placing a SDD to avoid irritating cortical tissue. The configuration of the haematoma does not appear to affect the postoperative seizure rate.

PMID:36097085 | DOI:10.1007/s10143-022-01858-5

Aesthetic Plast Surg. 2022 Sep 12. doi: 10.1007/s00266-022-03068-2. Online ahead of print.

ABSTRACT

BACKGROUND: The use of direct-to-implant subcutaneous breast reconstruction has increased over the last years. The goal of this systematic review is to deliver an updated review of the safety of this technique and its impact on quality of life. We also compare subcutaneous vs submuscular complications, through meta-analysis.

METHODS: Literature review through PubMed and Cochrane Library databases were performed by PRISMA criteria. Thirty-nine studies met inclusion criteria for subcutaneous review and 15 studies met inclusion criteria for meta-analysis. All included studies were evaluated for complications and answers to the BREAST-Q. Data were analysed using Microsoft Excel, IBM SPSS, and Cochrane RevMan.

RESULTS: In 2863 patients and 3988 breasts that undergone direct to implant subcutaneous breast reconstruction, 8,21% had rippling, 5,64% seroma, 1,74% hematoma, 3,40% infection, 3,01% wound dehiscence, 3,93% skin necrosis, 3,34% nipple-areolar-complex (NAC) necrosis, 3,07% capsular contracture, 0,00% animation deformity, and 3,83% an implant removal. Meta-analysis showed a statistically significant decrease in the odds ratio of animation deformity, a but statistically significant higher odds ratio of rippling. Subcutaneous and submuscular reconstructions had similar BREAST-Q scores.

CONCLUSIONS: Direct-to-implant subcutaneous breast reconstruction does not harm the patient’s quality of life, comparatively with submuscular, saving the pectoral muscle from dissection and preventing animation deformity, but increasing the risk of rippling.

LEVEL OF EVIDENCE III: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

PMID:36097081 | DOI:10.1007/s00266-022-03068-2

Sci Rep. 2022 Sep 12;12(1):15344. doi: 10.1038/s41598-022-19687-3.

ABSTRACT

This study aimed to examine the heart rate and blood lactate responses of female volleyball players during the match according to the player positions. A total of 24 senior female volleyball players (middle blocker (n = 6), setter (n = 6), spiker (n = 6), and libero (n = 6)) were monitored for heart rate and blood lactate before, during and after a volleyball match. The mean heart rate and blood lactate level of volleyball players were determined 117.2 ± 13.9 bpm, 2.7 ± 1.2 mmol/L during the match. Heart rate was statistically different between all playing positions except middle blockers vs. spikers (p < 0.05). The blood lactate levels between the groups were not statistically different. The setters have the lowest heart rate and the libero players have the highest during the match. All subjects have a heart rate 50-60% and below 50% of their heart rate maximum during more than half of the match duration. These current results can be used by coaches to determine a specific training load based on the heart rate and blood lactate differences between playing positions.

PMID:36097030 | DOI:10.1038/s41598-022-19687-3

Nat Commun. 2022 Sep 12;13(1):5350. doi: 10.1038/s41467-022-33093-3.

ABSTRACT

Age-related changes to the genome-wide DNA methylation (DNAm) pattern observed in blood are well-documented. Clonal hematopoiesis of indeterminate potential (CHIP), characterized by the age-related acquisition and expansion of leukemogenic mutations in hematopoietic stem cells (HSCs), is associated with blood cancer and coronary artery disease (CAD). Epigenetic regulators DNMT3A and TET2 are the two most frequently mutated CHIP genes. Here, we present results from an epigenome-wide association study for CHIP in 582 Cardiovascular Health Study (CHS) participants, with replication in 2655 Atherosclerosis Risk in Communities (ARIC) Study participants. We show that DNMT3A and TET2 CHIP have distinct and directionally opposing genome-wide DNAm association patterns consistent with their regulatory roles, albeit both promoting self-renewal of HSCs. Mendelian randomization analyses indicate that a subset of DNAm alterations associated with these two leading CHIP genes may promote the risk for CAD.

PMID:36097025 | DOI:10.1038/s41467-022-33093-3

Nat Commun. 2022 Sep 12;13(1):5239. doi: 10.1038/s41467-022-32848-2.

ABSTRACT

The blood and lymphatic vasculature networks are not yet fully understood even in mouse because of the inherent limitations of imaging systems and quantification methods. This study aims to evaluate the usefulness of the tissue-clearing technology for visualizing blood and lymphatic vessels in adult mouse. Clear, unobstructed brain/body imaging cocktails and computational analysis (CUBIC) enables us to capture the high-resolution 3D images of organ- or area-specific vascular structures. To evaluate these 3D structural images, signals are first classified from the original captured images by machine learning at pixel base. Then, these classified target signals are subjected to topological data analysis and non-homogeneous Poisson process model to extract geometric features. Consequently, the structural difference of vasculatures is successfully evaluated in mouse disease models. In conclusion, this study demonstrates the utility of CUBIC for analysis of vascular structures and presents its feasibility as an analysis modality in combination with 3D images and mathematical frameworks.

PMID:36097010 | DOI:10.1038/s41467-022-32848-2

Womens Health Issues. 2022 Sep 9:S1049-3867(22)00082-2. doi: 10.1016/j.whi.2022.07.004. Online ahead of print.

ABSTRACT

OBJECTIVE: Women incarcerated in local jails have pregnancy and sexual health needs, yet little information is available about what services are provided and how jail administrators prioritize this care. Our objective was to document jails’ provision of pregnancy and sexual health services in four states in the Midwest.

METHODS: We invited all jail administrators (N = 347) in Kansas, Missouri, Iowa, and Nebraska to participate in a web-based survey conducted from November 2017 to October 2018. We asked administrators which pregnancy and sexual health services they offered and to rate the importance of offering services. Results were analyzed using descriptive statistics and logistic regression.

RESULTS: The survey response rate was 55% (192/347). Jails most often provided pregnancy testing (n = 116 [60%]) and distribution of prenatal vitamins (n = 85 [44%]). Sexually transmitted infection treatment was offered at 23% of jails (n = 45). Larger, accredited jails located in urban areas and with high numbers of clinical providers on staff were more likely to provide sexual health services. Jails with privately contracted health care were more likely to provide pregnancy services compared with other entities providing medical care. The most prioritized sexual health service was sexually transmitted infection testing, with 39% of administrators believing it was important. Only 6% of administrators responded that contraception was important.

CONCLUSIONS: Local jails in the Midwest do not meet the basic reproductive and sexual health needs of women. Provision of these services is not a priority for jail administrators. Appropriate partnerships could engage administrators and increase the availability of services to meet the needs of women in jail.

PMID:36096980 | DOI:10.1016/j.whi.2022.07.004

Vaccine. 2022 Sep 9:S0264-410X(22)01077-5. doi: 10.1016/j.vaccine.2022.08.069. Online ahead of print.

ABSTRACT

BACKGROUND: Japan currently lacks a data platform that can support quantitative assessments of the causal relationships between vaccines and adverse events. This study describes the development and application of the Vaccine Effectiveness, Networking, and Universal Safety (VENUS) Study to facilitate such assessments.

METHODS: A database was created by linking public insurance enrollees’ claims data with vaccination records acquired from participating municipalities. To provide an overview of the study data, we produced descriptive statistics of sex, age, and vaccinations. We also conducted a pilot study using the database to assess influenza vaccine safety during the 2018/2019 season among older persons (≥65 years) residing in a single municipality.

RESULTS: Our database was created using data from approximately 1.12 million individuals in 7 municipalities between 2013 and 2020. The data during fiscal year 2018 included 853,016 individuals (male: 363,079, female: 489,937) with a median age of 70 years (interquartile range: 52-79). We obtained information on 17 vaccine types, including the pneumococcal vaccine and influenza vaccine. In the pilot study, we analyzed 48,723 vaccinated persons matched with 48,723 unvaccinated persons. The only adverse event that occurred in both groups was Bell’s palsy, which had an adjusted incidence rate ratio of 1.21 (95 % confidence interval: 0.48-3.07).

CONCLUSIONS: The VENUS Study is Japan’s first healthcare data platform that enables comparative assessments of vaccinated and unvaccinated persons in large samples covering all age groups. Efforts are underway to increase the number of participating municipalities and to generate evidence on vaccine effectiveness and safety.

PMID:36096969 | DOI:10.1016/j.vaccine.2022.08.069