Tag: nevin manimala

J Contemp Dent Pract. 2025 Nov 1;26(11):1050-1059. doi: 10.5005/jp-journals-10024-3971.

ABSTRACT

AIM: This study aims to examine the pro-apoptotic and anti-migratory effects of Berberine in KB-1 oral cancer cells and its role in ferroptosis via glutathione peroxidase4/six-transmembrane epithelial antigen of prostate3 (GPX4/STEAP3) signaling pathway.

MATERIALS AND METHODS: KB-1 cells were treated with Berberine, and cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes were analyzed via phase-contrast microscopy, while nuclear fragmentation was examined using acridine orange/ethidium bromide (AO/EtBr) staining. Molecular docking, gene expression analyses were performed to assess ferroptosis markers. Wound healing and migration assays evaluated Berberine’s effect on cell migration.

RESULTS: Berberine reduced cell viability (IC50: 22.2 μM) and induced morphological changes, including shrinkage and membrane blebbing. AO/EtBr staining confirmed nuclear fragmentation. Gene expression analysis showed ferroportin upregulation and GPX4, STEAP3, and transferrin receptor 1 (TFR1) downregulation, validated by western blotting. Molecular docking confirmed strong Berberine binding, particularly with GPX4 and STEAP3. Wound healing and migration assays showed significant migration suppression. All experimental data were statistically evaluated using the Student’s t-test and one-way ANOVA.

CONCLUSION: Berberine induces ferroptosis and inhibits KB-1 oral cancer cell proliferation and migration by modulating the ferroptosis signaling pathway, suggesting its potential as a novel therapeutic agent.

CLINICAL SIGNIFICANCE: By promoting iron-dependent cell death and suppressing migratory capacity, Berberine may overcome resistance to conventional therapies and reduce metastatic progression. Its natural origin and prior safety profile support drug repurposing opportunities, though challenges such as poor bioavailability and systemic toxicity must be addressed. These findings provide a foundation for biomarker-driven preclinical and early clinical evaluations. How to cite this article: Alassiri S. Berberine Induces Ferroptosis and Impairs Migration via Glutathione Peroxidase4/Six-transmembrane Epithelial Antigen of Prostate3 Signaling Pathway in Oral Cancer Cells. J Contemp Dent Pract 2025;26(11):1050-1059.

PMID:41368744 | DOI:10.5005/jp-journals-10024-3971

J Contemp Dent Pract. 2025 Nov 1;26(11):1047-1049. doi: 10.5005/jp-journals-10024-3892.

ABSTRACT

AIM AND BACKGROUND: In a controlled, simulated root canal environment, this study examined and contrasted the resistance to cyclic fatigue of three heat-treated nickel-titanium (NiTi) rotary file systems. The goal of this work was to find systems with greater fatigue endurance because cyclic fatigue is a primary mode of instrument failure in current endodontics.

MATERIALS AND METHODS: A total of 30 NiTi rotary instruments-10 from Hero Gold, NeoEndo Flex, and T-Pro-were put to the test. The dimensions of all instruments were the same: Size 25, taper 0.04, and length 21 mm. A specially designed device simulated a 60° canal curvature (8 mm from the tip) was used. Time to fracture (TTF) was recorded while the instruments were run at the torque and speed recommended by the manufacturer until they failed. A conventional procedure was used to calculate cycles to fracture (NCF). Measurements of fragment lengths were made, and one-way ANOVA and post hoc analysis were used to evaluate the significance of the findings.

RESULTS: Hero Gold outperformed NeoEndo Flex and T-Pro regarding fatigue resistance, as evidenced by considerably higher TTF and NCF values (p < 0.05). There was no statistically significant difference in fragment lengths between groups, suggesting that instruments consistently failed in the same places.

CONCLUSION: Hero Gold rotary files displayed the best fatigue resistance, indicating enhanced performance and durability in root canal applications.

CLINICAL SIGNIFICANCE: Understanding the differences in fracture resistance aids clinicians in selecting the appropriate instrumentation system for curved canals. How to cite this article: Boreak N. Assessment of Fatigue Resistance in Heat-treated Nickel-titanium Rotary Instruments with Single Canal Curvatures: An In Vitro Evaluation. J Contemp Dent Pract 2025;26(11):1047-1049.

PMID:41368743 | DOI:10.5005/jp-journals-10024-3892

J Contemp Dent Pract. 2025 Nov 1;26(11):1025-1034. doi: 10.5005/jp-journals-10024-3965.

ABSTRACT

AIM: To evaluate the role of erbium lasers (Er:YAG and Er,Cr:YSGG) as adjuncts in access flap periodontal surgery and their potential contribution to personalized, minimally invasive periodontal care.

BACKGROUND: Periodontal surgery has progressively evolved toward minimally invasive techniques prioritizing tissue preservation, enhanced healing, and reduced morbidity. Erbium lasers represent a significant advancement by offering precise tissue ablation, improved decontamination, and favorable patient experiences compared to conventional approaches.

REVIEW RESULTS: Evidence shows that erbium lasers achieve clinical outcomes comparable to or better than traditional flap surgery. Benefits include reduced postoperative discomfort, minimized gingival recession (GR), and enhanced patient satisfaction. While gains in clinical attachment level are modest yet statistically significant in some studies, patient-reported outcomes (PROs) consistently favor erbium-assisted interventions.

CONCLUSION: Erbium lasers enhance access flap surgery by supporting precision, soft tissue preservation, and improved patient-centered outcomes. Current data suggest they are valuable adjuncts, though long-term trials are still needed to establish standardized protocols.

CLINICAL SIGNIFICANCE: Integration of erbium lasers into periodontal flap surgery supports personalized and minimally invasive care, aligning with modern principles of precision periodontics and improving both clinical and PROs. How to cite this article: Neophytou C, Neofytou AM, Davidopoulou S, et al. Erbium Laser-assisted Access Flap Periodontal Surgery: Advancing Personalized Periodontal Care through Minimally Invasive Technologies. J Contemp Dent Pract 2025;26(11):1025-1034.

PMID:41368740 | DOI:10.5005/jp-journals-10024-3965

Aust N Z J Psychiatry. 2025 Dec 10:48674251389790. doi: 10.1177/00048674251389790. Online ahead of print.

ABSTRACT

OBJECTIVE: To estimate the proportions and correlates of Australian young people who consulted with health professionals or used services via digital technologies for their mental health in 2020-2022.

METHODS: Data from 16- to 24-year-olds (N = 1620) in the 2020-2022 Australian National Survey of Mental Health and Wellbeing were analysed to estimate proportions, population counts and unadjusted odds ratios of past-year health professional consultations and use of services via digital technology for mental health within geographic regions. Logistic regression models explored socio-economic, psychosocial and clinically meaningful correlates of past-year consultation in the full sample, metro subgroup and regional, rural and remote subgroup.

RESULTS: In total, 24.2% of Australian young people consulted with a health professional for their mental health in the past year. Of those with a probable 12-month Diagnostic and Statistical Manual of Mental Disorders (4th ed.; DSM-IV) mental health condition, 46.3% consulted a health professional. This proportion differed by sex (male: 34.2%, female: 55.6%) and symptom severity (mild: 20.0%, moderate: 47.8%, severe: 66.0%) but did not vary by geographic region. One-in-ten (9.9%) young people used other services via digital technologies for their mental health in the past year, doubling among those with a probable 12-month mental health condition (18.8%), and increasing with severity (mild or moderate: 14.2%, severe: 33.6%). Different factors were associated with service use in different regions.

CONCLUSION: Experiences of young people accessing mental health care in Australia differ by geographic region of residence, neighbourhood disadvantage, sex and disorder class. Australia’s mental health care system must facilitate diverse pathways to care that are responsive to young people’s needs and preferences.

PMID:41368729 | DOI:10.1177/00048674251389790

J Prosthodont. 2025 Dec 10. doi: 10.1111/jopr.70066. Online ahead of print.

ABSTRACT

PURPOSE: The success of removable partial dentures (RPDs) depends on well-designed abutments or surveyed crowns. Advances in computer-aided design and computer-aided manufacturing (CAD-CAM) technology have made all-ceramic materials viable alternatives to traditional ceramo-metal surveyed crowns, but their performance, particularly regarding internal fit and fracture resistance, requires further studies, especially with different rest seat preparations simulated on the abutment dies. This study evaluated the internal fit and fracture resistance of CAD-CAM ceramic crowns with wide and narrow rest seat designs to assess their potential as surveyed crowns for RPDs.

MATERIALS AND METHODS: Sixty mandibular premolar abutment dies were modified to simulate narrow and wide occlusal relief designs and were divided into six groups based on the design (wide/narrow) and material type: force-absorbed hybrid ceramics (HC), zirconium lithium silicate (ZLS), and lithium disilicate glass-ceramic (LDC). CAD-CAM ceramic crowns were fabricated, milled, and analyzed for internal fit using Geomagic Control X software. Fracture resistance in newton (N) was tested using a universal testing machine. Scanning electron microscopy (SEM) was employed to examine fracture morphology. Statistical analyses included two-way analysis of variance (ANOVA) and post hoc Tukey’s test (α = 0.05).

RESULTS: The LDC group had the highest fracture resistance, while ZLS and HC showed no significant difference [F(2) = 10.523, p < 0.001]. Internal fit did not significantly differ between materials [F(2) = 0.381, p = 0.686], though LDC had a slightly larger internal gap. Rest seat design significantly affected both fracture resistance and internal fit [F(1) = 68.581, p < 0.001; F(1) = 11.185, p = 0.002], with wide designs showing higher fracture resistance and narrow designs having larger internal gaps. SEM revealed crack propagation originating from rest seat bases, with longer crack lines observed in wide rest designs.

CONCLUSION: Rest seat design significantly influences the fracture resistance and internal fit of CAD-CAM ceramic crowns. Wide rest seat designs provide superior fracture resistance compared to narrow designs, while narrow seats exhibit larger internal gaps, potentially compromising restoration longevity.

PMID:41368727 | DOI:10.1111/jopr.70066

J Prosthodont. 2025 Dec 10. doi: 10.1111/jopr.70074. Online ahead of print.

ABSTRACT

PURPOSE: To compare the cost-efficiency of digitally designed and manufactured removable complete dentures (RCDs) with conventionally fabricated RCDs through a systematic review and meta-analysis.

METHODS: A comprehensive electronic and manual search was performed in PubMed, Embase, Web of Science, and Scopus up to February 10, 2025. Eligible studies included clinical trials and cohort studies comparing cost-related outcomes of digital and conventional RCD workflows. Primary outcomes were laboratory, clinical, and total costs; secondary outcomes included the number of treatment sessions. Meta-analyses were conducted using random-effects models. Risk of bias was evaluated using standardized tools.

RESULTS: Four retrospective studies and one prospective study, including 184 patients, met the inclusion criteria. No statistically significant differences were observed between digital and conventional workflows in laboratory costs (mean difference [MD]: -239.77 (2025 USD); p = 0.1063), clinical costs (MD: 74.39 (2025 USD); p = 0.4514), total costs (MD: -357.76 (2025 USD); p = 0.2577), or treatment sessions (MD: -1.47; p = 0.3514). Operator experience significantly influenced clinical costs (p < 0.0001) and the number of sessions (p = 0.0001).

CONCLUSION: Within the limitations of the available evidence, digital and conventional workflows for RCD fabrication demonstrated comparable cost-efficiency. Although digital workflows may reduce the number of sessions when performed by experienced clinicians, the current evidence is insufficient to establish a clear cost-efficiency advantage.

PMID:41368723 | DOI:10.1111/jopr.70074

J Dermatol. 2025 Dec 10. doi: 10.1111/1346-8138.70099. Online ahead of print.

ABSTRACT

Systemic amyloidosis is a multisystem disorder that requires histological confirmation of amyloid deposition in at least one organ for a definitive diagnosis. While biopsies of organs such as the myocardium provide high diagnostic accuracy, they are highly invasive and technically demanding. Thus, skin biopsies are frequently performed as a less invasive alternative. However, when skin biopsies fail to detect amyloid depositions, more invasive procedures-such as gastrointestinal or myocardial biopsies-are often required. Despite the clinical importance of improving diagnostic yield, few studies have systematically evaluated optimal skin biopsy techniques. Then, we conducted a retrospective observational study of 100 patients who underwent skin biopsies for suspected systemic amyloidosis at Kobe University Hospital between April 2014 and November 2024. In this study, two skin biopsy methods were analyzed: Punch biopsies from multiple random sites (1-5 sites) using Dermapunch (“punch (multiple-punch) biopsy”), and spindle-shaped biopsy of long axis approximately 10 mm or more (“incision (spindle-shaped) biopsy”). As a result, among 69 cases diagnosed as systemic amyloidosis based on amyloid detection in any organ, including the skin, 13 of 28 punch biopsies (46.4%) were positive, and 21 of 41 incision biopsies (51.2%) were positive. The difference in sensitivity was not statistically significant (p = 0.81), but incision biopsies showed a numerically higher sensitivity. Furthermore, fatty tissue was the most common amyloid deposition site, with a mean depth of 5.1 mm. In two cases, depositions were found at a depth of approximately 12 mm. In this study, no significant difference was observed in the diagnostic yield between the two biopsy methods. However, because amyloid depositions may occur deep within subcutaneous fat and incision biopsy enables deliberate and consistent sampling of this layer, it potentially improves diagnostic accuracy. We, therefore, recommend incision biopsy as the preferred method for diagnosing systemic amyloidosis.

PMID:41368718 | DOI:10.1111/1346-8138.70099

JMIR Public Health Surveill. 2025 Dec 9;11:e75818. doi: 10.2196/75818.

ABSTRACT

BACKGROUND: Cervical cancer (CC) is a major public health issue, accounting for approximately 350,000 deaths, around 7.5% of all female cancer deaths worldwide, in 2018. Human papillomavirus (HPV) is the most common virus infecting the reproductive system. Despite the high number of diagnosed cases of CC globally, prevention is possible. Vaccination against HPV is considered to be a primary prevention strategy, while cervical screening can also play a secondary prevention role.

OBJECTIVE: This study aimed to examine the knowledge and health beliefs of parents in Kuwait towards HPV and HPV vaccination in order to prepare for the development of a national policy on CC.

METHODS: A cross-sectional survey was conducted among a representative multistage sample of 538 parents and guardians of eligible children aged 12-17 years in Kuwait, yielding a response rate of 89%. The survey was structured using the health belief model. Analysis showed statistically significant links between knowledge, health beliefs, concepts, and vaccination intention.

RESULTS: Knowledge of HPV and HPV vaccination was low in our study population: 55.6% (n=297), 24.9% (n=133), and 19.5% (n=104) for poor, fair, and good knowledge, respectively. Parents of daughters scored lower on perceived susceptibility to HPV and were more likely to have a higher perception of barriers to HPV vaccination, even though fathers were more likely to believe their daughters were at risk. HPV vaccination has the stigma of promiscuity attached, even though half of the parents are willing to accept HPV vaccination if that recommendation comes from Uhealth officials or relatives. A greater proportion of parents with female children had a low perception of the severity of HPV infection compared to those with male children (n=154, 58.6% vs n=134, 49.4%; P=.043). Around 52% (n=278) of parents perceived a high benefit of HPV vaccination. Parents with a female child had a lower perception of HPV vaccine benefits compared to parents with a male child. The findings demonstrated that parents with higher levels of education were better informed about the use of HPV vaccines in controlling the illness. Parents with female children were 1.34 times more likely to act on the recommendation for HPV vaccination compared to parents with male children after a recommendation from an official source, such as doctors or healthcare professionals.

CONCLUSIONS: Recommendations for a Kuwaiti vaccination policy for HPV must take into consideration different knowledge levels of parents for groups with different educational levels, as well as the stigma of promiscuity and other barriers, and various health beliefs regarding susceptibility for daughters and sons, respectively.

PMID:41368717 | DOI:10.2196/75818

Clin J Pain. 2025 Dec 10. doi: 10.1097/AJP.0000000000001351. Online ahead of print.

ABSTRACT

OBJECTIVE: To compare the relative efficacy of common non-pharmacological treatments for migraine and to determine the optimal dosage for physical exercise.

METHODS: We searched four databases up to January 2025 for randomized controlled trials of non-pharmacological interventions for migraine. A multilevel network meta-analysis, integrated with a dose-response analysis, was conducted to compare intervention efficacy and determine the optimal exercise dosage. Treatments were ranked by the Surface Under the Cumulative Ranking curve. Two independent reviewers extracted data and assessed the risk of bias.

RESULTS: Fifty-nine randomized controlled trials involving 10,020 participants (78.1%, female) were included. Neuromodulation techniques were most effective (Hedges’g=-0.61, 95% Credible Interval: -0.89 to -0.33), followed by physical exercise (Hedges’g=-0.42, 95% Credible Interval: -0.67 to -0.18) and mindfulness meditation (Hedges’g=-0.38, 95% Credible Interval: -0.63 to -0.12). The dose-response analysis for exercise indicated that while 100 metabolic equivalent (MET)-minutes per session was statistically effective, a minimum of 110 MET-minutes per session was required to achieve the Minimal Clinically Important Difference. Efficacy reached an optimal therapeutic plateau at 250-300 MET-minutes per session, achievable with 3-5 weekly sessions of 30-40 minutes.

DISCUSSION: Neuromodulation, physical exercise, and mindfulness meditation are promising non-pharmacological therapies for migraine. For physical exercise, a minimum dose of 110 MET-minutes per session is needed for clinically significant effects, with an optimal therapeutic window at 250-300 MET-minutes per session. Due to the low quality of primary evidence, these findings warrant cautious interpretation and require future validation.

PMID:41368711 | DOI:10.1097/AJP.0000000000001351

J Magn Reson Imaging. 2025 Dec 10. doi: 10.1002/jmri.70194. Online ahead of print.

ABSTRACT

BACKGROUND: Pathophysiological changes affect tissue cell composition and density. For example, neurodegenerative disorders and brain tumors are associated with cell loss and abnormal accumulation, respectively. In these scenarios, if monitored and tracked, tissue cellularity might be used to inform clinical diagnosis and management.

PURPOSE: To propose and evaluate a new marker of tissue cellularity, called susceptibility-Derived Cellularity Index (χDCI), that would be readily available for clinical applications with fast acquisition and at high resolution.

STUDY TYPE: Retrospective study.

POPULATION/SUBJECTS: 24 healthy subjects (7/17 M/F, 70 ± 11 years) and 21 patients with IDH-wild type glioblastoma (16/5 M/F, 65 ± 8 years).

FIELD STRENGTH/SEQUENCE: 3 T MRI sequences including 3D T1w pre- and post-contrast agent injection, 3D T2w, 3D FLAIR, 3D multi-echo gradient recalled echo, 2D diffusion weighted imaging.

ASSESSMENT: χDCI was computed based on parameters estimated with DECOMPOSE-QSM. The Neurite Density Index (NDI) was estimated with the NODDI model. T1w images were used for region of interest (ROIs) segmentations with FreeSurfer (i.e., cortical gray matter, white matter, thalamus, caudate, putamen, pallidum, hippocampus and amygdala). For the patients with glioblastoma, regions of contrast enhancement, necrosis, and edema were also included in the analysis.

STATISTICAL TESTS: Pearson’s correlation analysis between mean χDCI and NDI values in the ROIs was carried out separately for the two cohorts of participants (significance level = 0.05, after correction for multiple comparisons).

RESULTS: Significant correlations were observed between χDCI and NDI in white matter (r = 0.56) and putamen (r = 0.69) for the healthy participants. Significant positive correlations were also found in white matter (r = 0.6), pallidum (r = 0.48), putamen (r = 0.79), thalamus (r = 0.64) and edema (r = 0.69) for the patient cohort.

DATA CONCLUSION: χDCI is proposed as a marker of tissue cellularity. The significant associations between χDCI and NDI in several regions investigated in the present study support the potential of χDCI as a proxy of intracellular volume fraction.

TECHNICAL EFFICACY: Stage 1.

PMID:41368710 | DOI:10.1002/jmri.70194