Tag: nevin manimala

J Low Genit Tract Dis. 2023 Dec 18. doi: 10.1097/LGT.0000000000000793. Online ahead of print.

ABSTRACT

OBJECTIVE: This study aimed to investigate the characteristics and screening history of vaginal intraepithelial neoplasia (VaIN) or vaginal cancer and compare the sensitivity of cytology and human papillomavirus (HPV) tests on the cervix against vaginal and cervical high-grade squamous intraepithelial lesion or cancer.

METHODS: This study included patients who underwent colposcopy-directed biopsy and were diagnosed with VaIN or vaginal cancer from February 2013 to November 2022. Clinical information was obtained from the medical records of the department. Statistical analysis was performed on SPSS 26.0 (IBM Corp, Armonk, NY) using t test, chi-square, and Fisher exact tests.

RESULTS: A total of 1,166 patients were included in this study. The median age of VaIN2+ patients was 50.5 years, whereas VaIN1 reported a median age of 42.1 years old, p < .001. This study reported that VaIN was significantly and positively correlated with cervical lesions (r = 0.244). The high-risk HPV (hr-HPV) detection rate was 88.2% (858/973) in VaIN and 95.2% in VaIN2+. Human papillomavirus 16 was the most prevalent HPV type in VaIN2+, which accounted for 54.9%, followed by HPV58 (19.5%), HPV52 (15.2%), HPV51 (12.2%), and HPV18 (11.0%). The sensitivity of hr-HPV and cytology tests on the cervix for detecting VaIN2+ was 94.7% and 83.4%, respectively. Both tests were not significantly different from detecting cervical intraepithelial neoplasia 2+.

CONCLUSIONS: Human papillomavirus 16 is the dominant HPV type in vaginal precancer lesions. Cervical cancer screening has similar sensitivity for VaIN2+ as for cervical intraepithelial neoplasia 2+, with hr-HPV testing showing higher sensitivity than cytology.

PMID:38109483 | DOI:10.1097/LGT.0000000000000793

PLoS Comput Biol. 2023 Dec 18;19(12):e1011722. doi: 10.1371/journal.pcbi.1011722. Online ahead of print.

ABSTRACT

Recent experimental evidence strongly supports that three-dimensional (3D) long-range enhancer-promoter (E-P) interactions have important influences on gene-expression dynamics, but it is unclear how the interaction information is translated into gene expression over time (4D). To address this question, we developed a general theoretical framework (named as a 4D nucleome equation), which integrates E-P interactions on chromatin and biochemical reactions of gene transcription. With this equation, we first present the distribution of mRNA counts as a function of the E-P genomic distance and then reveal a power-law scaling of the expression level in this distance. Interestingly, we find that long-range E-P interactions can induce bimodal and trimodal mRNA distributions. The 4D nucleome equation also allows for model selection and parameter inference. When this equation is applied to the mouse embryonic stem cell smRNA-FISH data and the E-P genomic-distance data, the predicted E-P contact probability and mRNA distribution are in good agreement with experimental results. Further statistical inference indicates that the E-P interactions prefer to modulate the mRNA level by controlling promoter activation and transcription initiation rates. Our model and results provide quantitative insight into both spatiotemporal gene-expression determinants (i.e., long-range E-P interactions) and cellular fates during development.

PMID:38109463 | DOI:10.1371/journal.pcbi.1011722

Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2023 Dec 18;44(3):27-39. doi: 10.2478/prilozi-2023-0044. Print 2023 Dec 1.

ABSTRACT

Introduction: Studies determined that age and associated comorbidities are associated with worse outcomes for COVID-19 patients. The aim of the present study is to examine previous electronic health records of SARS-CoV-2 patients to identify which chronic conditions are associated with in-hospital mortality in a nationally representative sample. Materials and Methods: The actual study is a cross-sectional analysis of SARS-CoV-2 infected patients who were treated in repurposed hospitals. The study includes a cohort of patients treated from 06-11-2020 to 15-03-2021 for COVID-19 associated pneumonia. To examine the presence of comorbidities, electronic health records were examined and analyzed. Results: A total of 1486 in-patients were treated in the specified period, out of which 1237 met the criteria for case. The median age of the sample was 65 years. The overall in-hospital mortality in the sample was 25.5%, while the median length of stay was 11 days. From whole sample, 16.0% of the patients did not have established diagnoses in their electronic records, while the most prevalent coexisting condition was arterial hypertension (62.7%), followed by diabetes mellitus (27.3%). The factors of age, male gender, and the number of diagnoses showed a statistically significant increase in odds ratio (OR) for in-hospital mortality. The presence of chronic kidney injury was associated with the highest increase of OR (by 3.37) for in-hospital mortality in our sample. Conclusion: The study reaffirms the findings that age, male gender, and the presence of comorbidities are associated with in-hospital mortality in COVID-19 treated and unvaccinated patients. Our study suggests that chronic kidney injury showed strongest association with the outcome, when adjusted for age, gender, and coexisting comorbidities.

PMID:38109453 | DOI:10.2478/prilozi-2023-0044

Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2023 Dec 18;44(3):49-55. doi: 10.2478/prilozi-2023-0046. Print 2023 Dec 1.

ABSTRACT

The main goals of this research are to determine the developmental tendency of salmonellosis in the Republic of North Macedonia and to determine the index of dynamics and the rate of decline/increase of salmonellosis in the Republic of North Macedonia. The research covers the period from 01.01.2012 until 31.12.2022. The data used to implement this study were obtained from the Institute of Public Health of the Republic of North Macedonia. The research included 2711 subjects who were sick with salmonellosis from 2012 to 2022. The following statistical methods of work are used: linear trend, dynamic index with a constant base, dynamic index with a variable base (chain index), rate of decline, and rate of increase. The results of the study suggest that in the period 2012-2022, the highest number of patients (n=373) and the highest incidence (18/100000) of salmonellosis were registered in 2018, and the lowest in 2020 (n=119, I=5.8/100000). From the linear trend values, it can be concluded that salmonellosis in the Republic of North Macedonia shows a decreasing tendency. The biggest increase in salmonellosis in the Republic of North Macedonia was in 2015 by 71% compared to 2014. The biggest decrease in salmonellosis in the Republic of North Macedonia was in 2020 by 48% compared to 2019. From the study, it can be concluded that food operators and manufacturers are observing the procedures of the HACCP control system for food safety, leading to a possible decrease in salmonellosis.

PMID:38109452 | DOI:10.2478/prilozi-2023-0046

Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2023 Dec 18;44(3):79-84. doi: 10.2478/prilozi-2023-0050. Print 2023 Dec 1.

ABSTRACT

In psychology, alienation is a condition of being separated or marginalized from other individuals or some larger segment of society. Self-efficacy is an individual’s belief in their capacity to act in ways necessary to reach specific goals. This study was conducted to investigate the possible connectivity between psychological alienation and feelings of self-efficiency among young male and female adults. The study was conducted in April 2023, among 167 young people, aged 18-25 (M=22.28 ± 1.7SD). For this purpose, we used the Scale for psychological alienation and General self-efficacy. Results showed that there is a significant negative correlation between alienation and self-efficacy, meaning that the higher psychological alienation is, the lower self-efficacy is (t=-0.627; <0.001). There is a statistically significant difference between young male and female adults regarding alienation (t=-5.592; <0.001) e. g. males are reporting to be more alienated than females. Also, there is a statistically significant difference between young male and female adults regarding feeling of self-efficacy (t=4.692; <0.001) e.g. males feel less effective than females and in the scale for measuring meaninglessness (t=-2.966; 0.003) e. g. males feel more meaninglessness than females. In conclusion, psychological alienation and self-efficacy are correlated and there are gender differences regarding the two measured variables, in favor of the females.

PMID:38109447 | DOI:10.2478/prilozi-2023-0050

Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2023 Dec 18;44(3):17-26. doi: 10.2478/prilozi-2023-0043. Print 2023 Dec 1.

ABSTRACT

Introduction: Central nervous system (CNS) tumours represent a significant public health issue worldwide, and their incidence and distribution vary across different populations. Although studies on CNS tumours have been conducted in various countries, there is a lack of information regarding their patterns in Macedonia. Therefore, this study is aimed at investigating the distribution, histopathological types and subtypes and demographic features of CNS tumours in our country. Materials and Methods: A cross sectional study was conducted using the electronic database of the Institute of Pathology – Medical Faculty, University “Ss. Cyril and Methodius” in Skopje which contains data from 3286 received and analysed surgical specimens, mainly from the University Clinic of Neurosurgery in Skopje, and a smaller number of surgical specimens from the University Surgical Centre “St. Naum Ohridski” in Skopje between 2012 and 2022. The collected and analysed data includes patient age, sex and histopathological types and subtypes of the tumours. Results: The majority of CNS tumours were diagnosed in adults aged between 50-70, with a male to female ratio of 1.5:1. The most common location of the tumours was the cerebrum, followed by the pituitary gland and cerebellum. The most frequent histological groups were gliomas, with glioblastoma as the most common diagnosis, followed by meningiomas. Conclusion: Following a detailed and thorough review of the CNS tumours in our study, we can conclude that the R. of Macedonia follows global statistics and trends regarding brain tumours.

PMID:38109442 | DOI:10.2478/prilozi-2023-0043

PLoS Genet. 2023 Dec 18;19(12):e1011074. doi: 10.1371/journal.pgen.1011074. Online ahead of print.

ABSTRACT

Linkage disequilibrium (LD) is a fundamental concept in genetics; critical for studying genetic associations and molecular evolution. However, LD measurements are only reliable for common genetic variants, leaving low-frequency variants unanalyzed. In this work, we introduce cumulative LD (cLD), a stable statistic that captures the rare-variant LD between genetic regions, which reflects more biological interactions between variants, in addition to lack of recombination. We derived the theoretical variance of cLD using delta methods to demonstrate its higher stability than LD for rare variants. This property is also verified by bootstrapped simulations using real data. In application, we find cLD reveals an increased genetic association between genes in 3D chromatin interactions, a phenomenon recently reported negatively by calculating standard LD between common variants. Additionally, we show that cLD is higher between gene pairs reported in interaction databases, identifies unreported protein-protein interactions, and reveals interacting genes distinguishing case/control samples in association studies.

PMID:38109434 | DOI:10.1371/journal.pgen.1011074

Int J Prosthodont. 2023 Dec 18;36(6):781-786. doi: 10.11607/ijp.7984.

ABSTRACT

PURPOSE: To compare the accuracy of milled versus 3D-printed complete denture bases and teeth and to assess the position of the teeth in relation to the corresponding denture bases.

MATERIALS AND METHODS: Two different manufacturing techniques were used in this study. In Group A, 10 complete dentures were digitally designed and fabricated by milling prepolymerized blocks of polymethyl methacrylate (PMMA). In Group B, 10 complete dentures were digitally designed and fabricated using a 3D-printing technique. The accuracy of the maxillary and mandibular denture bases and teeth and the positions of the teeth in relation to the corresponding denture bases were evaluated using Geomagic Control X software (Oqton). Data were presented as mean and SD values. Statistical analysis of the resultant data was performed using Student t test. The significance level was set at P ≤ .05.

RESULTS: With regard to denture bases, lower surface deviation values were found in the maxillary and mandibular milled denture bases (Group A), with values of 0.158 ± 0.024 and 0.117 ± 0.022, respectively. However, regarding the denture teeth, lower surface deviation values were found for printed teeth (Group B), with values of 0.18 ± 0.016 for the maxillary teeth and 0.153 ± 0.02 for the mandibular teeth. For positioning of the teeth in relation to the corresponding denture bases, the values were 0.4 ± 0.08 for the maxillary teeth and 1.003 ± 0.027 for the mandibular teeth.

CONCLUSIONS: The milling technique yields complete denture bases with superior accuracy, whereas printing technology produces denture teeth with better accuracy and positioning in the corresponding denture bases.

PMID:38109400 | DOI:10.11607/ijp.7984

Int J Prosthodont. 2023 Dec 18;36(6):748-753. doi: 10.11607/ijp.6440.

ABSTRACT

PURPOSE: To examine and compare the fracture strength of implant-cemented fixed partial denture (FPD) prostheses fabricated with digital vs conventional chairside methods.

MATERIALS AND METHODS: Three groups of seven specimens each were produced: group A (3D printing); group B (milling); and group C (conventional chairside manufacturing), which served as a control. All groups were cemented to standard implant abutments placed in artificial bone blocks. Fracture strength testing was performed using a universal testing machine. Statistical analysis of the resultant maximum forces was performed using SPSS version 25 software (Mann- Whitney U test, P < .05).

RESULTS: The mean fracture load value of the group A FPDs was 260.14 N ± 28.88, for group B was 663.57 N ± 140.55, and for group C was 266.65 N ± 63.66.

CONCLUSIONS: Milled provisional FPDs showed a higher fracture resistance compared to 3D-printed and control groups. However, no such difference could be detected between the 3D-printed and control groups.

PMID:38109396 | DOI:10.11607/ijp.6440

Int J Prosthodont. 2023 Dec 18;36(6):738-747. doi: 10.11607/ijp.7771.

ABSTRACT

PURPOSE: To assess the impact of fatigue loading on the internal and marginal fit of CAD/CAM-fabricated endocrowns for restoring endodontically treated molars using different machinable blocks.

MATERIALS AND METHODS: A total of 72 mandibular first molars were prepared using a standardized method and were divided into four groups (n = 18), each restored with a different CAD/CAM material: group V = polymer-infiltrated ceramic (Vita Enamic); group K = partially stabilized tetragonal zirconia (Katana); group E = lithium disilicate ceramic (IPS e.max CAD; and group B = polyetheretherketone (BioHPP). Endocrowns were subjected to thermomechanical fatigue loading. Internal and marginal adaptation of the endocrowns were examined at 66 points using the sectioning technique and a stereomicroscope. Data were analyzed using one-way ANOVA and Tukey post hoc test (α = .05).

RESULTS: Statistical tests showed that adhesive cementation resulted in significantly increased marginal and internal gap values in all regions in all four tested groups (P < .001). After thermomechanical fatigue loading, all regions in groups B and K showed a statistically significant difference, while no significant differences were found in the regions in group V (P > .05). In group E, the marginal (F = 71.00) and pulpal (F = 28.065) regions showed statistically significant differences (P < .001).

CONCLUSION: Polymer-infiltrated ceramics showed the lowest gap even after thermomechanical fatigue loading and may therefore provide enhanced clinical survival of the restored tooth, favoring the use of this material for fabricating endocrown restorations.

PMID:38109395 | DOI:10.11607/ijp.7771