Tag: pubmed

Breast Cancer Res Treat. 2025 Jul 18. doi: 10.1007/s10549-025-07781-9. Online ahead of print.

ABSTRACT

PURPOSE: Previous studies have demonstrated that ductal carcinoma in situ (DCIS) component often exhibits higher HER2 expression than the invasive component when assessed by immunohistochemistry, while some other studies showed concordant HER2 expression between these two components. In this study, we used our high-sensitivity HER2 (HS-HER2) quantitative immunofluorescence assay to compare HER2 expression in IDC and co-existing DCIS and correlate with clinicopathologic characteristics.

METHODS: We included 36 IDC + DCIS cases from the Yale Pathology department. DCIS was classified according to the three-tier nuclear grading system: low (grade 1), intermediate (grade 2), and high (grade 3) nuclear grade. Invasive carcinoma was graded according to the modified Bloom-Richardson histologic grading system. Cases were divided into two groups: low to intermediate-grade DCIS (G1-2) with co-existing invasive carcinoma (n = 26) and high-grade DCIS (G3) with co-existing invasive carcinoma (n = 10). Separate regions of interest for IDC and DCIS were annotated by two board-certified pathologists. Serial sections of FFPE tumor specimens were used to accurately measure the HER2 protein expression by the HS-HER2 assay in attomole/mm² unit and the acquisition by QuPath v.04 with the Qymia extension.

RESULTS: Low to intermediate-grade DCIS expressed higher HER2 levels (4295 ± 449 amol/mm²) than co-existing invasive carcinoma (2880 ± 413 amol/mm²). Similarly, high-grade DCIS expressed higher HER2 levels (4953 ± 700 amol/mm²) than co-existing invasive carcinoma (3560 ± 688 amol/mm²). Neither of these trends toward lower expression levels in the IDC were statistically significant. Additionally, no significant statistic difference was noted between low to intermediate-grade DCIS versus high-grade DCIS or between their corresponding co-existing invasive carcinomas in this cohort.

CONCLUSION: Using the HS-HER2 assay, our results demonstrated comparable HER2 expression levels in DCIS and paired invasive carcinoma regardless of histopathological grade or HER2 immunohistochemical score. These findings contributed to a more nuanced understanding of HER2 biology in early breast carcinogenesis and may inform future biomarker-driven therapeutic strategies.

PMID:40679712 | DOI:10.1007/s10549-025-07781-9

Sleep Breath. 2025 Jul 18;29(4):248. doi: 10.1007/s11325-025-03389-x.

ABSTRACT

BACKGROUND: The increasing use of e-cigarettes and the decline in sleep duration among young people are growing public health concerns. While cigarette smoking has been linked to shorter sleep duration, less is known about the effects of e-cigarettes. This study explores the association between exclusive e-cigarette use and sleep duration among US young adults aged 18-24 who only use e-cigarettes.

METHODS: We analyzed data from 26,943 young adults in the 2022 Behavioral Risk Factor Surveillance System survey. Sleep duration was categorized as short (< 7 h/24 hours), normal (7-9 h), or long (> 9 h). We excluded participants using any tobacco/nicotine products other than e-cigarettes and restricted the sample to 4,553 young adults with complete data. Logistic regression models assessed e-cigarette use status (i.e., current use, past use, and non-use) in relation to sleep duration, adjusting for demographics, current substance use, lifestyle factors, and health conditions.

RESULTS: Overall, 31.9% of young adults experienced short sleep duration, 63.2% reported normal sleep, and 4.9% had long sleep. Current e-cigarette users had a higher prevalence of short sleep duration (44.6%) than past users (33.0%) and non-users (27.4%; p-value < 0.001). Weighted analyses indicated that current e-cigarette use was significantly associated with short sleep duration (adjusted OR = 1.82; 95%CI: 1.30-2.54), but no significant associations were found with long sleep duration (all p-values > 0.05).

CONCLUSIONS: Current exclusive e-cigarette use is associated with short sleep duration among US young adults. Efforts to reduce e-cigarette use in this population may contribute to improved sleep health.

PMID:40679707 | DOI:10.1007/s11325-025-03389-x

Trop Anim Health Prod. 2025 Jul 18;57(7):307. doi: 10.1007/s11250-025-04550-0.

ABSTRACT

This study investigated the effects of Lactobacillus plantarum (LAC) and its cell-free supernatant (CFS) on vaginal health and reproductive performance in ewes. The ewes were synchronized using fluorogestone acetate (FGA) or medroxyprogesterone acetate (MPA) impregnated intravaginal sponges. A total of 196 Merino ewes were randomly assigned to four groups in a 2 × 2 factorial design. Intravaginal sponges remained for 14 days, and vaginal discharge, sponge weight change, estrus response, and pregnancy rates were evaluated. No adverse health effects were observed following intravaginal probiotic treatment. There was a tendency (p = 0.07) for higher sponge loss in the FGA group (13.2%) compared to the MPA group (5.7%). On the day of sponge removal, 90.8% of ewes exhibited vaginal discharge. Although not statistically significant, mean vaginal discharge scores were lower in LAC-treated groups compared to CFS-treated groups. While L. plantarum had no significant effect on vaginal discharge scores in the MPA group (40.7% in MPA + LAC vs. 41.7% in MPA + CFS for score 2; p > 0.05), it significantly reduced purulent/hemorrhagic discharge in the FGA group (29.7% in FGA + LAC vs. 47.6% in FGA + CFS; p < 0.05). Estrus initiation occurred significantly earlier in the FGA group (36.8 ± 1.9 h) compared to the MPA group (49.1 ± 1.2 h; p < 0.01), although neither the probiotic treatment nor its interaction with progestagen type significantly influenced estrus timing. Pregnancy rates were significantly higher in the FGA + LAC (71.7%) and FGA + CFS (75.6%) groups compared to the MPA + LAC (49.0%) and MPA + CFS (50.0%) groups (p < 0.01). Our findings suggest that FGA-based synchronization improves pregnancy rates, while treatment with L. plantarum may enhance vaginal health, providing a potential non-antibiotic approach for reproductive management.

PMID:40679696 | DOI:10.1007/s11250-025-04550-0

Eur J Pediatr. 2025 Jul 18;184(8):489. doi: 10.1007/s00431-025-06313-3.

ABSTRACT

Serial lung recruitment assessment in neonates with moderate-to-severe neonatal acute respiratory distress syndrome (NARDS) is crucial. However, current methods involve ionizing radiation or invasiveness, which limits their serial use in neonates. This study evaluated the feasibility of rib-indexed quantitative lung ultrasound (LUS) as a radiation-free alternative for monitoring lung aeration in neonates with moderate-to-severe NARDS on surfactant therapy combined with prone position. A prospective observational study enrolled 35 term neonates with moderate-to-severe NARDS. Lung recruitment was assessed via anterior-posterior approach rib-indexed quantitative LUS and posteroanterior chest X-ray (CXR) before and 6 h after combined surfactant therapy and prone position. Following the intervention, it demonstrated a significant reduction in the LUS aeration score, from a pre-intervention median of 18 points (IQR 16, 22) to a post-intervention median of 15 points (IQR 12, 20) (P < 0.001). In contrast, the decrease in the CXR score (pre-intervention median 3 (IQR 3, 4) vs. post-intervention median 2 (IQR 2, 3)) did not reach statistical significance (P = 0.059). Posterior approach rib-indexed quantitative LUS showed high concordance with posteroanterior CXR in determining the rib level of the pulmonary-diaphragmatic interface (ICC > 0.95, kappa > 0.94, P < 0.001). No adverse events occurred during the LUS assessments.Conclusion: Posterior approach rib-indexed quantitative LUS is a reliable and non-invasive modality for real-time lung recruitment assessment in neonates with NARDS. It significantly detected improved lung aeration following surfactant therapy combined with prone position, whereas CXR failed to demonstrate a statistically significant improvement. Posterior approach rib-indexed quantitative LUS can also determine the rib level of the pulmonary-diaphragmatic interface, similarly to posteroanterior CXR. The superior sensitivity and safety of rib-indexed quantitative LUS offer a clinically valuable and innovative alternative for dynamic monitoring of lung recruitment in neonatal critical care. Future multi-centre studies should integrate CT validation to confirm broader applicability.Trial registration: The trial was prospectively registered with the Chinese Clinical Trial Registry (ChiCTR2300074652) on August 11, 2023.

PMID:40679694 | DOI:10.1007/s00431-025-06313-3

Trop Anim Health Prod. 2025 Jul 18;57(7):304. doi: 10.1007/s11250-025-04553-x.

NO ABSTRACT

PMID:40679686 | DOI:10.1007/s11250-025-04553-x

J Robot Surg. 2025 Jul 18;19(1):403. doi: 10.1007/s11701-025-02557-1.

ABSTRACT

Robotic surgery is increasingly prevalent in thoracic surgery. Studies of specific kinematic data on outcomes are missing. The object of this study was to examine correlations between years in practice, robotic experience, and kinematic (motion) data on complications and readmissions. Kinematic data from the first lymph node dissection of anatomic robotic lung resections were combined with data from a prospectively maintained single institution database. Kinematic data included arm movement speed, economy of motion, and camera movement. Lobectomies and segmentectomies were matched and propensity-score weighted with inverse-probability treatment weights. Pearson’s correlations, between years in practice, robotic experience, and kinematic data; and logistic regression; between years in practice and robotic experience on complications and readmissions were done. Lobectomies, 42, and segmentectomies, 31, from 2022 to 2023 were included. After matching, lymph nodes sampled were greater with lobectomies than segmentectomies, but other data were well matched; nodes sampled with lobes 13.4 vs 7.6 with segments, p < 0.001. Years in practice and robotic experience were not correlated with complications or readmissions. Significant correlations were noted between kinematic data and robotic experience. Length of stay had a negative correlation with robotic experience, -0.16, p < 0.001. No odds ratios were significant. In this small series, decreased length of stay was correlated with increased robotic experience which was a stronger outcome determinant than practice years. The complications and readmissions were not correlated with either measure. Increasing robotic experience may help optimize patient care but additional data are needed to establish usefulness of specific kinematic data.

PMID:40679677 | DOI:10.1007/s11701-025-02557-1

Sleep Breath. 2025 Jul 18;29(4):247. doi: 10.1007/s11325-025-03378-0.

ABSTRACT

PURPOSE: The relationship between obesity hypoventilation syndrome (OHS) and traffic accidents has not been previously studied. We aimed to determine the frequency of traffic accidents and the associated risk factors among people with OHS and compare with obstructive sleep apnoea (OSA) individuals.

METHODS: We screened patients diagnosed with OSA and/or OHS in our sleep laboratory between 2015 and 2023. Patients who had a driver’s license, had been driving for at least five years, at least three days a week, and drove more than 5000 km/year and had not received positive airway pressure treatment prior to their diagnosis were included. Polysomnographic data, the Epworth Sleepiness Scale (ESS), the Berlin Sleep Questionnaire were collected from the records. The Fatigue Assessment Scale (FAS) and inattention scores before the diagnosis, driving information and accident numbers from the three years before the diagnosis to the phone call were evaluated.

RESULTS: A total of 121 patients (OHS/OSA: 43/78, F/M: 5/116, age: 46.9 ± 9.7 years, body mass index [BMI]: 32.5 ± 5.7 kg/m²) were included. ESS, FAS, apnoea-hypopnea index (AHI), oxygen desaturation index (ODI) and percentage of total sleep time with SpO₂ less than 90% were significantly greater in OHS patients (p = 0.012, p = 0.028, p = 0.003, p = 0.002 and p = 0.021, respectively). The frequency of accidents was greater in OHS patients than in OSA patients (46.5% vs. 26.9%, p = 0.029). In the multivariate analysis, only BMI was an independent factor (OR: 1.185, 95% CI: 1.005-1.396, p = 0.044).

CONCLUSION: Traffic accidents were more common in OHS patients than in OSA patients, with obesity as the primary independent risk factor.

PMID:40679659 | DOI:10.1007/s11325-025-03378-0

Food Funct. 2025 Jul 18. doi: 10.1039/d5fo00873e. Online ahead of print.

ABSTRACT

The rising prevalence of type 2 diabetes (T2D) demands effective dietary strategies. High-phenolic extra virgin olive oil (EVOO) has been proposed as a functional food with antidiabetic properties. This study evaluates the effects of a high-phenolic EVOO from native Galician olives on glycemic control (primary outcome), lipid profile, anthropometric and blood pressure parameters (secondary outcomes) in adults with T2D. A 24-week experimental, prospective, randomized, parallel, long-term controlled trial was conducted with 116 T2D subjects. Participants were randomly allocated either to a Control group advised to minimize consumption of EVOO (preferring refined olive oil blends) or an Interventional group receiving 30 mL day^-1 of Galician phenolic-rich EVOO. Glycemic biomarkers, lipid profile, anthropometric indices, and blood pressure were assessed at baseline, 12 and 24 weeks. After 24 weeks, the Interventional group demonstrated significant reductions in insulin resistance (HOMA IR). No significant changes were observed in lipid profile or blood pressure in either group, while both groups exhibited modest reductions in body weight and body mass index (BMI). Although beneficial effects were particularly pronounced among individuals with obesity (reductions in fasting glucose, estimated average glucose and glycosylated hemoglobin (HbA1c)) and insulin-resistant participants (reductions in fasting insulin and HOMA IR), these subgroup analyses lacked sufficient statistical power and must be interpreted cautiously. These findings highlight the therapeutic potential of phenolic-rich EVOOs as a complementary dietary strategy for managing T2D.

PMID:40678895 | DOI:10.1039/d5fo00873e

Int Nurs Rev. 2025 Sep;72(3):e70071. doi: 10.1111/inr.70071.

ABSTRACT

AIMS: This study explores how workplace violence influences community nurses’ job burnout, specifically identifying the chain-mediating role of perceived stress and job satisfaction in this relationship.

BACKGROUND: Existing research highlights the negative impacts of workplace violence on nurses’ physical health, mental well-being, and job attitudes. However, community nurses remain understudied, particularly regarding the sequential mediating roles of perceived stress and job satisfaction between workplace violence and burnout.

METHODS: This cross-sectional study followed STROBE guidelines and recruited 814 community nurses from 75 communities in southwest China. Data were collected via Questionnaire Star using five validated instruments to assess job burnout, job satisfaction, workplace violence exposure, and perceived stress. Descriptive statistics and Pearson’s correlations were analyzed using SPSS 26.0, while structural equation modeling tested the chain-mediating effects of perceived stress and job satisfaction in the workplace violence-burnout relationship among community nurses.

RESULTS: Workplace violence was positively correlated with job burnout. Perceived stress and job satisfaction partially mediated this relationship and functioned as sequential mediators in the violence-burnout pathway.

CONCLUSION: Workplace violence exacerbates community nurses’ job burnout through a chain-mediating pathway involving elevated perceived stress and subsequent diminished job satisfaction.

IMPLICATIONS FOR NURSING AND NURSING POLICY: Managers should prioritize workplace violence against community nurses by enhancing violence prevention training to improve staff’s risk assessment and emergency response skills. Additionally, standardized violence reporting systems, trauma assessment protocols, workplace environment improvements, and psychological support mechanisms should be established to create a comprehensive prevention-response framework.

PMID:40678877 | DOI:10.1111/inr.70071

Int Nurs Rev. 2025 Sep;72(3):e70067. doi: 10.1111/inr.70067.

ABSTRACT

AIMS: To assess home disaster preparedness levels of individuals with sickle cell disease to determine education and resource needs and inform policy. To evaluate the association between healthcare provider discussion of preparedness and level of preparedness.

BACKGROUND: Research demonstrates that extreme weather events and public health emergencies result in increased hospital visits for people with sickle cell disease, yet little is known about home disaster preparedness knowledge and actions of these individuals.

METHODS: Cross-sectional study of US adults diagnosed with sickle cell disease. The independent variable was the healthcare provider discussion of household disaster preparedness. The dependent variable was the Household Emergency Preparedness Instrument’s General Preparedness score. Participants self-reported their preparedness actions and disaster supplies through an online survey. Descriptive statistics were analyzed, along with a Welch t test, to examine the associations between the study variables. This study was reported according to the STROBE checklist.

RESULTS: The average General Preparedness score of this sample (N = 32) indicated that the majority of participants were not considered well prepared for disasters. Higher scores were found among participants who had discussions with their healthcare providers about how to prepare for disasters.

DISCUSSION: The sample’s average General Preparedness score was higher than other patient populations assessed with this instrument, possibly due to routine discussions about medical emergency preparedness that individuals with sickle cell disease receive from healthcare providers.

CONCLUSION: Home disaster preparedness levels should be improved to decrease disaster-related morbidity and mortality.

IMPLICATIONS FOR NURSING PRACTICE AND POLICY: Nurses should develop or advocate for home disaster preparedness lessons, community interventions, and public policies to meet the unique needs of community members with sickle cell disease before, during, and after disasters.

PMID:40678875 | DOI:10.1111/inr.70067