Tag: pubmed

BMC Bioinformatics. 2023 Jan 3;24(1):2. doi: 10.1186/s12859-022-05114-x.

ABSTRACT

BACKGROUND: Gene-based association tests provide a useful alternative and complement to the usual single marker association tests, especially in genome-wide association studies (GWAS). The way of weighting for variants in a gene plays an important role in boosting the power of a gene-based association test. Appropriate weights can boost statistical power, especially when detecting genetic variants with weak effects on a trait. One major limitation of existing gene-based association tests lies in using weights that are predetermined biologically or empirically. This limitation often attenuates the power of a test. On another hand, effect sizes or directions of causal genetic variants in real data are usually unknown, driving a need for a flexible yet robust methodology of gene based association tests. Furthermore, access to individual-level data is often limited, while thousands of GWAS summary data are publicly and freely available.

RESULTS: To resolve these limitations, we propose a combination test named as OWC which is based on summary statistics from GWAS data. Several traditional methods including burden test, weighted sum of squared score test [SSU], weighted sum statistic [WSS], SNP-set Kernel Association Test [SKAT], and the score test are special cases of OWC. To evaluate the performance of OWC, we perform extensive simulation studies. Results of simulation studies demonstrate that OWC outperforms several existing popular methods. We further show that OWC outperforms comparison methods in real-world data analyses using schizophrenia GWAS summary data and a fasting glucose GWAS meta-analysis data. The proposed method is implemented in an R package available at https://github.com/Xuexia-Wang/OWC-R-package CONCLUSIONS: We propose a novel gene-based association test that incorporates four different weighting schemes (two constant weights and two weights proportional to normal statistic Z) and includes several popular methods as its special cases. Results of the simulation studies and real data analyses illustrate that the proposed test, OWC, outperforms comparable methods in most scenarios. These results demonstrate that OWC is a useful tool that adapts to the underlying biological model for a disease by weighting appropriately genetic variants and combination of well-known gene-based tests.

PMID:36597047 | DOI:10.1186/s12859-022-05114-x

J Speech Lang Hear Res. 2023 Jan 3:1-9. doi: 10.1044/2022_JSLHR-21-00688. Online ahead of print.

ABSTRACT

PURPOSE: A critical review was completed to evaluate replication of aphasia treatments that have been vetted and accepted on the American Speech-Language-Hearing Association Practice Portal.

METHOD: The electronic databases Academic Search Premier, ProQuest Central, CINAHL Complete, and ERIC were searched for relevant articles using treatment names as keywords. Coders compared stimuli, material, design, and statistical analysis to pilot treatment approaches. Each study was coded as direct, conceptual, failed, or no replication.

RESULTS: Eighteen treatment approaches were selected for this review. A total of 188 articles met the inclusion criteria. Results revealed that 14 out of 18 treatment approaches were somehow replicated. Direct replications as the most valuable replication type for affirming previously found results were represented in only 1.5% of all studies (3/188). Failed direct replication were at 2% overall (4/188). Conceptual replications were more common across treatment approaches, but also represented at a low level with 22.8% (43/188). The majority of studies were coded as no replication attempt with 73.4%.

DISCUSSION: A critical factor in developing an evidence-based practice is the existence of replicated results for treatment. Replication evaluates the reproducibility of an author’s or authors’ research methodology and resulting outcomes and helps to ensure that observed treatment effects are reproducible. For an evidence-based treatment to be implemented or used in any clinical setting, it must be one that can be replicated. Direct and conceptual replications of aphasia treatment approaches were found to be alarmingly low considering the importance of replication in our field. It is recommended that replication should become more valued and mainstreamed in aphasia research. A replication database that compiles and maintains treatment manuals for replication purposes can increase the accessibility and acceptability of replications for researchers.

PMID:36596266 | DOI:10.1044/2022_JSLHR-21-00688

JMIR Form Res. 2022 Dec 27. doi: 10.2196/43639. Online ahead of print.

ABSTRACT

BACKGROUND: RamaCovid is a mobile health (mHealth) education system that provides the Thai population with information about COVID-19 and self-risk assessment. RamaCovid has a chatbot system that provides automatic conversations (available 24 hours per day) and a live chat function that allows users to directly communicate with health professionals (available 4 hours per day in the evening). The system consists of (1) COVID-19 vaccine information, (2) self-care after vaccination, (3) frequently asked questions, (4) self-risk assessment, (5) hospital finding, (6) contact number finding, and (7) live chat with a health professional.

OBJECTIVE: This study investigates the use of and satisfaction with the RamaCovid system.

METHODS: Overall, 400 people were recruited via RamaCovid by broadcasting an infographic about the study. Questionnaires collected demographic data, users’ experiences of RamaCovid, and the use of and satisfaction with the system. The questions were answered using a 5-point Likert scale. Descriptive statistics were used to describe the participant characteristics and their use of and satisfaction with the RamaCovid system. The Mann-Whitney U test was performed to examine the difference in use and satisfaction between the adult and older adult groups.

RESULTS: The participants showed high use of and satisfaction with the RamaCovid system. They used the information to take care of themselves and their family, and they gained information about their COVID-19 risk. The users were satisfied with the system because the information was easy to understand, trustworthy, and up to date. However, the older adult group had lower use of and satisfaction with the system compared to the adult group.

CONCLUSIONS: RamaCovid is an example of the successful implementation of mHealth education. It was an alternative way to work with the call center during the COVID-19 pandemic and increased access to health information and health care services. Providing ongoing updated information, improving the attractiveness of the media information, and the age group difference are important issues for further system development.

PMID:36596210 | DOI:10.2196/43639

J Obstet Gynaecol. 2022 Oct;42(7):2680-2683. doi: 10.1080/01443615.2022.2081799. Epub 2022 Jun 4.

ABSTRACT

The primary aim of this study was to investigate the potential impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on maternal and foetal Doppler findings. Doppler ultrasound findings were compared in 40 pregnant women diagnosed with COVID-19 disease who required hospitalisation (group 1) and 30 healthy pregnant women (group 2). Maternal characteristics and birth histories were recorded. Body mass index, gestational week at birth, type of delivery, oligihydroamnios, pre-term birth (<37 weeks), low birth weight (<10 percentile), perinatal death and f1st and 5th minute Apgar scores were recorded. Birth weights and foetal biophysical profile (BPP) scores in group 1 were significantly lower than those in group 2. There was a statistically significant between-group difference in the umbilical artery pulsatility index (PI), umbilical artery resistive index (RI), middle cerebral artery (MCA) PI, MCA RI, mean uterine artery (UtA) PI, mean UtA RI and cerebroplacental ratio (CPR), the parameters used to evaluate foetal-maternal blood flow. In the pregnant group diagnosed with COVID-19 and hospitalised, all foetal-maternal Doppler indicators of foetal-maternal blood flow were impaired, and birth weights and BPP scores in these patients were statistically significantly lower than those in the healthy controls.Impact statementWhat is already known on this subject? Foetal and maternal vascular malperfusion characterised by decidual arteriopathy have been reported in pathologies of placentas from pregnant women with SARS-CoV-2 infection.What the results of this study add? It was determined that COVID-19 disrupted foetal and maternal blood flow.What the implications are of these findings for clinical practice and/or further research? Foetal biometric measurements and foetal Doppler may be useful in the follow-up of perinatal outcomes in pregnant women with COVID-19.

PMID:36596209 | DOI:10.1080/01443615.2022.2081799

Food Res Int. 2023 Jan;163:112228. doi: 10.1016/j.foodres.2022.112228. Epub 2022 Dec 5.

ABSTRACT

Actinidia arguta, an edible berry plant with high nutritional values, has been widely used in Asian countries as a food and traditional medicinal herb. The well-recognized health-promoting properties of A. arguta were associated with its bioactive components in its different botanical parts. To rapidly screen and identify chemical components and simultaneously determine the potential metabolites from different parts of A. arguta, UPLC-Q-TOF-MS^E coupled with UNIFI platform and multivariate statistical analysis approach was established in this study. As a result, a total of 107 components were identified from the four different parts of A. arguta, in which 31 characteristic chemical markers were discovered among them, including 12, 8, 6, and 5 compounds from the fruits, leaves, roots, and stems, respectively. These results suggested that the combination of UPLC-Q-TOF-MS^E and metabolomic analysis is a powerful method to rapidly screen characteristic markers for the quality control of A. arguta.

PMID:36596158 | DOI:10.1016/j.foodres.2022.112228

Food Res Int. 2023 Jan;163:112225. doi: 10.1016/j.foodres.2022.112225. Epub 2022 Nov 23.

ABSTRACT

The commercial quality of foxtail millet grain (Setaria italica L.) includes appearance quality, functional quality, and cooking and eating quality, which directly determine whether consumers will purchase the product. We studied the relationship between ecological factors and commercial quality attributes of foxtail millet “Jingu 21” from twelve production areas. The results showed that altitude, latitude, and diurnal temperature range were negatively correlated with b*, total flavones content (TFC), setback (SB), consistence (CS) and pasting temperature (PTM), but positively correlated with L/B and breakdown (BD). In contrast, average temperature, average precipitation, average humidity, available nitrogen, phosphorus, and potassium had positive effects on 1,000-grain weight (KGW), b*, TFC, CS, and PTM and had a negative impact on L/B and BD. Climate factors had a greater effect on the commercial quality of foxtail millet than soil factors, and the influence of climatic factors was particularly obvious in the early and middle growth periods. The multivariate equation between ecological factors and the comprehensive score of foxtail millet commercial quality is Y = 1,159.745-4.496X₁ (altitude) + 19.529X₅ (≥10℃ effective accumulated temperature) – 166.327X₁₀ (organic matters). In conclusion, high temperature and precipitation are conducive to high quality appearance and the accumulation of functional substances, while a high diurnal temperature range and high soil nutrients are conducive to the formation of cooking and eating quality. The impact of ecological factors on foxtail millet quality is complicated and it is essential to select a cultivation site that is matched to the intended use of the foxtail millet being produced.

PMID:36596154 | DOI:10.1016/j.foodres.2022.112225

Medicine (Baltimore). 2022 Dec 30;101(52):e32588. doi: 10.1097/MD.0000000000032588.

ABSTRACT

Traumatic dental injury is one of the common injuries seen in the emergency department. To determine the distribution features of emergency traumatic dental injuries in Xi’an, China, data from the medical records of 623 patients (aged 6-78 years) were evaluated and analyzed according to age, gender, etiology, factors predisposing teeth to injury, and types of teeth traumatized. The patient records of 397 males (63.7%) and 226 females (36.3%), with a 1.8:1 male-to-female ratio, were included. The total number of traumatized teeth was 1475. The highest frequency of dental trauma was found in the 19- to 30-year age group (30.7%). The greatest frequency of dental trauma injuries was in May (13%). Over one-third of patients with traumatic injuries to permanent teeth (n = 218, 35%) injured 2 permanent teeth. The most commonly affected teeth were the maxillary central incisors (59.3%). The most common type of trauma was concussion (20.4%). There was statistically significant difference among the type composition ratio of age groups (P < .05). The most common causes of dental trauma were falls (50.2%). There was statistically significant difference among the etiological composition ratio of age groups (P < .05). Combined injuries were present in 120 teeth in 103 patients (16.5%). The epidemiological characteristics of dental trauma in Xi’an shows that the occurrence of dental trauma is closely related to the age, sex, time, type of injury, and cause of injury. There exists cross-correlation among the epidemiological factors. The most common type of trauma is concussion and the most common cause of dental trauma is fall. High incidence of combined injuries needs extra attention.

PMID:36596078 | DOI:10.1097/MD.0000000000032588

Medicine (Baltimore). 2022 Dec 30;101(52):e32597. doi: 10.1097/MD.0000000000032597.

ABSTRACT

Lower extremity revascularization (LER) for peripheral artery disease in elderly patients is associated with a high risk of perioperative morbidity and mortality. This study aimed to a conduct retrospective review and propensity score matching analysis to determine whether the use of regional anesthesia (RA) instead of general anesthesia (GA) in geriatric patients undergoing LER for peripheral artery disease results in improved short-term mortality and health outcomes. We reviewed medical records of 1271 patients aged >65 years who underwent LER at our center between May 1998 and February 2016. According to the anesthesia method, patients were grouped in the GA and RA groups. The primary outcome was short-term mortality (7-day and 30-day). The secondary outcomes were 5-year survival rate, intraoperative events, postoperative morbidity, and postoperative length of stay. A propensity score-matched cohort design was used to control for potentially confounding factors including patient demographics, comorbidities, American Society of Anesthesiologists physical status, and preoperative medications. After propensity score matching, 722 patients that received LER under GA (n = 269) or RA (n = 453) were identified. Patients from the GA group showed significantly higher 7-day mortality than those from the RA group (5.6% vs 2.7% P = .048); however, there was no significant difference in 30-day mortality between the groups (GA vs RA: 6.3% vs 3.6%, P = .083). The 5-year survival rate and incidence of arterial and central venous catheter placement or intraoperative dopamine and epinephrine use were significantly higher in the GA group than in the RA group (P < .05). In addition, the frequency of immediate postoperative oxygen therapy or mechanical ventilation support was higher in the GA group (P < .05). However, there was no difference in the postoperative cardiopulmonary and cerebral complications between the 2 groups. These results suggest that RA can reduce intraoperative hemodynamic support and provide immediate postoperative respiratory intensive care. In addition, the use of RA may be associated with better short-term and 5-year survival rates in geriatric patients undergoing LER.

PMID:36596067 | DOI:10.1097/MD.0000000000032597

Medicine (Baltimore). 2022 Dec 30;101(52):e32366. doi: 10.1097/MD.0000000000032366.

ABSTRACT

BACKGROUND: Approximately half of people with heart failure have chronic kidney disease (CKD). Tolvaptan is reported to be effective in treating heart failure. However, the safety and efficacy of its use in patients with CKD is uncertain. In this study, we conducted a protocol for systematic review and meta-analysis to investigate the efficacy and safety of tolvaptan on patients with heart failure and CKD.

METHODS: This study protocol has been registered in the PROSPERO and the registration number is CRD42022368148. The consent of this protocol report is based on the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) 2015 statement guidelines. We will include randomized controlled trials related to tolvaptan in patients with heart failure and CKD. Two research members will electronically and independently search 4 English databases (EMBASE, PubMed, National Guideline Clearinghouse, and Cochrane Central Register of Controlled Trials) and 4 Chinese databases (Chinese Biomedical Literature Database, Chinese National Knowledge Infrastructure, Wanfang Database, and VIP Database) from their inception to November 2022. The risk of bias in each included study will be assessed utilizing the Cochrane Collaboration’s risk of bias tool. All statistical analyses will be conducted using the software program Review Manager version 5.3.

RESULTS: The results of this systematic review will be published in a peer-reviewed journal.

CONCLUSION: This review can provide convincing evidence to help clinicians make decisions when dealing with heart failure and CKD.

PMID:36596041 | DOI:10.1097/MD.0000000000032366

Medicine (Baltimore). 2022 Dec 30;101(52):e32429. doi: 10.1097/MD.0000000000032429.

ABSTRACT

No significant progress has been made in the study of orthopedic surgical site infection (SSI) after different orthopedic surgery, and the analysis and prevention of risk factors for orthopedic SSI urgently need to be solved. A total of 154 patients underwent orthopedic surgery from April 2018 to December 2020. General information such as gender, age, marriage, diagnosis, surgical site, and anesthesia method was recorded. Statistical methods included Pearson chi-square test, univariate and multivariate logistic regression analyses, and receiver operating characteristic (ROC) curves. Based on Pearson’s chi-square test, sex (P = .005), age (P = .027), marriage (P = .000), diagnosis (P = .034), and surgical site (P = .000) were significantly associated with SSI after orthopedic surgery. However, in the multiple linear regression analysis, only the surgical site (P = .035) was significantly associated with SSI after orthopedic surgery. In terms of multivariate logistic regression level, surgical site (odds ratio [OR] = 1.568, P = .039) was significantly associated with SSI. ROC curves were constructed to determine the effect of the surgical site on SSI after different orthopedic surgery (area under the curve [AUC] = 0.577, 95% CI = 0.487-0.0.666). In summary, the surgical site is an independent risk factor for SSI after orthopedic surgery, and “trauma” is more likely to develop SSI than spine, arthrosis, and others.

PMID:36596026 | DOI:10.1097/MD.0000000000032429