Tag: pubmed

J Am Soc Cytopathol. 2021 Feb 22:S2213-2945(21)00026-0. doi: 10.1016/j.jasc.2021.02.003. Online ahead of print.

ABSTRACT

OBJECTIVE: The objective of this study was to investigate the feasibility of implementing videos captured by static telecytological applications for remote cytological diagnosis of fine needle aspiration (FNA) specimens from salivary gland lesions.

METHODS: The current study was performed on 102 specimens from patients referred to the Alpha Prolipsis Cytopathology Department for preoperative evaluation of salivary gland lesions. In all cases, surgical excision followed the initial cytological diagnosis. (benign lesions, 11; benign neoplasms, 68; malignant neoplasms, 23). Videos were transferred via file transfer protocol to password-protected accounts for remote review by three independent cytopathologists. In addition to diagnosis, reviewers commented on overall digital video quality. Contributor’s and reviewer’s diagnoses were collected, recorded and statistically evaluated.

RESULTS: Statistical evaluation of cytological diagnoses detected no significant difference in diagnostic accuracy between the diagnoses proffered on the basis of precaptured videos and conventional slides. The overall interobserver agreement was ranging from substantial to almost perfect with κ values of 0.71-0.89.

CONCLUSIONS: Videos production by static telecytology applications can be used as an alternative method for telecytological diagnosis of salivary glands FNAs. Videos of salivary glands FNAs can be used for accurate diagnosis, educational and second opinion purposes,. They can also be used for archiving, teleconsultation and educational purposes, improving the performance of the already existing static telecytology stations and small cytology departments’ quality indices.

PMID:33707150 | DOI:10.1016/j.jasc.2021.02.003

Sleep Med. 2021 Feb 9:S1389-9457(21)00052-6. doi: 10.1016/j.sleep.2021.01.035. Online ahead of print.

ABSTRACT

OBJECTIVE/BACKGROUND: The aim of this study was to examine the relationship between overnight consolidation of implicit statistical learning with spindle frequency EEG activity and slow frequency delta power during non-rapid eye movement (NREM) sleep in obstructive sleep apnea (OSA).

PATIENTS/METHODS: Forty-seven OSA participants completed the experiment. Prior to sleep, participants performed a reaction time cover task containing hidden patterns of pictures, about which participants were not informed. After the familiarisation phase, participants underwent overnight polysomnography. 24 h after the familiarisation phase, participants performed a test phase to assess their learning of the hidden patterns, expressed as a percentage of the number of correctly identified patterns. Spindle frequency activity (SFA) and delta power (0.5-4.5 Hz), were quantified from NREM electroencephalography. Associations between statistical learning and sleep EEG, and OSA severity measures were examined.

RESULTS: SFA in NREM sleep in frontal and central brain regions was positively correlated with statistical learning scores (r = 0.41 to 0.31, p = 0.006 to 0.044). In multiple regression, greater SFA and longer sleep onset latency were significant predictors of better statistical learning performance. Delta power and OSA severity were not significantly correlated with statistical learning.

CONCLUSIONS: These findings suggest spindle activity may serve as a marker of statistical learning capability in OSA. This work provides novel insight into how altered sleep physiology relates to consolidation of implicitly learnt information in patients with moderate to severe OSA.

PMID:33707093 | DOI:10.1016/j.sleep.2021.01.035

Arch Pediatr. 2021 Mar 8:S0929-693X(21)00024-5. doi: 10.1016/j.arcped.2020.12.013. Online ahead of print.

ABSTRACT

It is well established today that an optimal vitamin D intake plays a crucial role in the constitution of optimal osseous mass during childhood, and hence in the prevention of the osteoporosis in adults. The prevalence of the vitamin D deficiency is increasing globally and Algeria is no exception in this regard. Our study is the first to be carried out with healthy children of preschool age in North Africa.

AIMS: The study aimed to evaluate the vitamin D status of a pediatric population, during all four seasons of the year, living in the north of Algeria, as well as to estimate the prevalence of vitamin D deficiency, identifying the potential risk factors.

MATERIAL AND METHODS: This was an analytic and cross-sectional study carried out between March 2014 and March 2016 with healthy preschool infants from an urban environment in the town of Hussein Dey. A total of 1016 infants aged 9-72 months were included during this period. The consensual threshold value was 20ng/mL.

RESULTS: The sex ratio was of 1.47 (535 boys/481 girls) and the average age of the children was 36.5±1.79 months. The daily average calcium ratio was 395±23 mg/with food contributions in vitamin D at an estimated average of 164 UI/day (4.1μg/day). The average concentration of the total 25-OHD for all four seasons of the year was 18.6±10.4ng/mL with an average rate of parathyroid hormone (PTH) of 30.9±14.6pg/mL. There was a highly significant inverse correlation between the serum level of vitamin D and PTH (r=-0.57; P=0.0001), the point of inflection was situated at 34.1ng/mL. The prevalence of vitamin D deficiency follows a seasonal variation that is statistically significant (P=0.0001), and the prevalence is higher during the autumn-winter period. The risk factors identified by multiple logistic regression were autumn-winter season (OR: 7; 95% CI: 3-11; P=0.001), age less than 24 months (OR: 3.8; 95% CI: 3.4-4.4; P=0.0001), high body mass index (OR: 2; 95% CI: 1.2-3.2; P=0.3), darker skin pigmentation (OR: 2.8; 95% CI: 2.2-5.2; P=0.001), duration of sunlight exposure less than 15min (OR: 6.1; 95% CI: 3.6-10.2; P=0.0001), low socioeconomic status (OR: 3.9; 95% CI: 1.5-4.3; P=0.01), calcium intake lower than 500 mg/day (OR: 2.5; 95% CI: 1.8-6; P=0.001), and a weekly dietary intake of vitamin D lower than 200 UI (OR: 2.6; 95% CI: 1.6-4.2; P=0.02).

DISCUSSION: No studies have been conducted in north Africa or Algeria concerning healthy preschool children; however, this population has a rapid growth rate and deserves special attention. The prevalence of vitamin D deficiency in the children of this study was higher than that reported in studies of children of the same age living in Europe or America, despite the fact that Algeria is closer to the equator (36° latitude north).

CONCLUSION: The changes experienced by Algerian society and the shorter exposure of the population to the sun call for more efforts regarding the detection and treatment of vitamin D deficiency, as well as an update of the vitamin D supplementation schedule.

PMID:33707102 | DOI:10.1016/j.arcped.2020.12.013

J Med Imaging Radiat Sci. 2021 Mar 8:S1939-8654(21)00036-9. doi: 10.1016/j.jmir.2021.02.004. Online ahead of print.

ABSTRACT

PURPOSE: The purpose of this project was to assess factors that may influence variability in the pre-treatment kilovoltage cone beam computed tomography (kV CBCT) image matching process for lung stereotactic body radiation therapy (SBRT).

METHODS AND MATERIALS: Pre-treatment CBCT and planning CT data sets of previously-treated lung SBRT patients were gathered and anonymized from four radiotherapy centers in Alberta. Eight radiation therapists (RTTs) and four radiation oncologists (ROs) were recruited from the same four cancer centers for image matching. Identical data sets were provided to each user, but the order of image sets was randomized independently for each user to remove any learning bias. Inter-user variabilities were then investigated as functions of various factors, including image origin (source institution/machine), user’s institution (local matching protocol), profession (RTT vs. RO), years of experience and image quality (presence/absence of added noise).

RESULTS: Very little variation in image matching between different users was observed. The mean differences from the consensus means for different image sets were less than 1 mm in all directions, and cases that exceeded 3 mm (i.e. clinically significant differences) were extremely rare. Image origin, user’s institution, and profession (RTT vs. RO) didn’t lead to any meaningful clinical differences, while image quality didn’t introduce any statistically significant differences. In addition, no discernible trend was seen between user’s experience and deviation from the user mean. Overall, no meaningful differences in inter-user variabilities for the different factors investigated were found in this study.

CONCLUSIONS: There appears to be an adequate standardization across the province of Alberta in terms of CBCT image matching process. No clinically significant differences were observed as functions of various factors investigated in this study. Consistency in matching between RTTs and ROs in this study suggests that RTTs do not need systematic RO approval of their lung CBCT match. It should be noted that RTTs at the centers in this study receive comprehensive training in CBCT-based image matching.

PMID:33707110 | DOI:10.1016/j.jmir.2021.02.004

Eur Urol. 2021 Mar 8:S0302-2838(21)00145-7. doi: 10.1016/j.eururo.2021.02.025. Online ahead of print.

ABSTRACT

BACKGROUND: Risk stratification for localized renal cell carcinoma (RCC) relies heavily on retrospective models, limiting their generalizability to contemporary cohorts.

OBJECTIVE: To introduce a contemporary RCC prognostic model, developed using prospective, highly annotated data from a phase III adjuvant trial.

DESIGN, SETTING, AND PARTICIPANTS: The model utilizes outcome data from the ECOG-ACRIN 2805 (ASSURE) RCC trial.

OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: The primary outcome for the model is disease-free survival (DFS), with overall survival (OS) and early disease progression (EDP) as secondary outcomes. Model performance was assessed using discrimination and calibration tests.

RESULTS AND LIMITATIONS: A total of 1735 patients were included in the analysis, with 887 DFS events occurring over a median follow-up of 9.6 yr. Five common tumor variables (histology, size, grade, tumor necrosis, and nodal involvement) were included in each model. Tumor histology was the single most powerful predictor for each model outcome. The C-statistics at 1 yr were 78.4% and 81.9% for DFS and OS, respectively. Degradation of the DFS, DFS validation set, and OS model’s discriminatory ability was seen over time, with a global c-index of 68.0% (95% confidence interval or CI [65.5, 70.4]), 68.6% [65.1%, 72.2%], and 69.4% (95% CI [66.9%, 71.9%], respectively. The EDP model had a c-index of 75.1% (95% CI [71.3, 79.0]).

CONCLUSIONS: We introduce a contemporary RCC recurrence model built and internally validated using prospective and highly annotated data from a clinical trial. Performance characteristics of the current model exceed available prognostic models with the added benefit of being histology inclusive and TNM agnostic.

PATIENT SUMMARY: Important decisions, including treatment protocols, clinical trial eligibility, and life planning, rest on our ability to predict cancer outcomes accurately. Here, we introduce a contemporary renal cell carcinoma prognostic model leveraging high-quality data from a clinical trial. The current model predicts three outcome measures commonly utilized in clinical practice and exceeds the predictive ability of available prognostic models.

PMID:33707112 | DOI:10.1016/j.eururo.2021.02.025

Burns. 2021 Feb 27:S0305-4179(21)00056-5. doi: 10.1016/j.burns.2021.02.020. Online ahead of print.

ABSTRACT

PURPOSE: This study examines the differences in parent-perceived and patient-reported quality of life (QoL) among young adult burn patients three years after injury and the factors affecting these differences.

METHOD: The sample comprised 35 burn patients from the Formosa Fun Coast Water Park dust explosion and their parents. The study was conducted from June 2016 to August 2018. We used self-report questionnaires to collect socio-demographic data, the adapted Chinese version of the Burn Specific Health Scale-Brief, and the Impact of Events Scale for Burn.

RESULTS: The analysis indicated that simple abilities recovered the fastest, while body image recovered the slowest. The variation trends of these factors were similar but parents’ scores were lower than patients’ scores. Parents’ post-traumatic stress disorder (PTSD) scores were higher than that of patients, but were not statistically significant. Parents’ gender and PTSD levels and patients’ burn area affected differences in parent-perceived QoL among patients. PTSD levels were significantly higher among mothers.

CONCLUSIONS: For parents, PTSD is a common response to their children experiencing burn injuries. Parents’ observations of warning signs enable early medical intervention. Establishing a family-centered care plan, providing psychological support for both parents and patients, and forming a continuous care system with efficient communication can support patients’ return to society.

PMID:33707088 | DOI:10.1016/j.burns.2021.02.020

Ultrasound Med Biol. 2021 Mar 8:S0301-5629(21)00058-2. doi: 10.1016/j.ultrasmedbio.2021.02.001. Online ahead of print.

ABSTRACT

In this study we compared three different microbubble-based approaches to the delivery of a widely used chemotherapy drug, gemcitabine: (i) co-administration of gemcitabine and microbubbles (Gem+MB); (ii) conjugates of microbubbles and gemcitabine-loaded liposomes (GemlipoMB); and (iii) microbubbles with gemcitabine directly bound to their surfaces (GembioMB). Both in vitro and in vivo investigations were carried out, respectively, in the RT112 bladder cancer cell line and in a murine orthotopic muscle-invasive bladder cancer model. The in vitro (in vivo) ultrasound exposure conditions were a 1 (1.1) MHz centre frequency, 0.07 (1.0) MPa peak negative pressure, 3000 (20,000) cycles and 100 (0.5) Hz pulse repetition frequency. Ultrasound exposure produced no significant increase in drug uptake either in vitro or in vivo compared with the drug-only control for co-administered gemcitabine and microbubbles. In vivo, GemlipoMB prolonged the plasma circulation time of gemcitabine, but only GembioMB produced a statistically significant increase in cleaved caspase 3 expression in the tumor, indicative of gemcitabine-induced apoptosis.

PMID:33707089 | DOI:10.1016/j.ultrasmedbio.2021.02.001

Ultrasound Med Biol. 2021 Mar 8:S0301-5629(21)00056-9. doi: 10.1016/j.ultrasmedbio.2021.01.031. Online ahead of print.

ABSTRACT

The incidence of muscle spasticity is high in patients with diseases of the central nervous system. This condition leads to significant limitations in movement and impaired functional capacities. Muscle spasticity manifests as changes in the mechanical properties of the muscles. This muscle disorder is generally assessed using qualitative methods, whose validity, reliability and sensitivity are questionable. In recent years, ultrasound elastography (USE) has been used as a non-invasive technique for characterizing the stress response and mechanical properties of individual muscles in the evaluation of spasticity. This article presents a systematic review of the USE techniques, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) declaration guidelines, used for the evaluation of muscle stiffness caused by spasticity. The articles selected in this study were evaluated using the Quality Appraisal of Diagnostic Reliability (QAREL) tool. Mainly, studies on the assessment of spasticity involving the evaluation of muscle stiffness with USE techniques (i.e., shear-wave elastography [SWE] or compression elastography [CE]) were selected. Then, common topics related to the assessment of spastic muscles and the statistical results of these studies were classified. Of 21 articles, nine used only CE, 10 used only SWE and two used both techniques. In the studies, statistically significant differences were observed in the measurements of muscle stiffness between the paretic and non-paretic sides as well as between patients and healthy patients. The clinical measurements obtained, devices used and assessment and probe positions for both techniques were discussed. The most frequently studied muscles were the biceps (n = 7) and gastrocnemius (n = 11). On applying the QAREL tool, we found that only two studies showed compliance at 80%-90%, seven studies at 50%-70% and the remaining 12 at 10%-40%. The results showed that USE techniques have limitations in spasticity assessment, such as subjectivity because of the lack of standardized protocols. A deficit of studies on intra-operator and inter-operator measurements indicates that this technique is not yet mature for spasticity diagnosis, although it is a promising diagnostic tool for designing treatment plans and monitoring the effectiveness of therapeutic modalities.

PMID:33707090 | DOI:10.1016/j.ultrasmedbio.2021.01.031

Cognition. 2021 Mar 8:104646. doi: 10.1016/j.cognition.2021.104646. Online ahead of print.

ABSTRACT

Infants’ capacity to extract statistical regularities from sequential information is impressive and well documented. However, statistical learning’s underlying mechanism remains mostly unknown, and its role in language acquisition is still under debate. To shed light on these issues, here we address the question of which information human subjects extract and encode after familiarisation with a continuous sequence of stimuli and its dependence on the type of segmentation cues and on the stimuli modality. Specifically, we investigate whether adults and 5-month-old infants learn the syllables’ co-occurrence in the stream or generate a representation of the Words that include syllables’ ordinal position. We test if subtle pauses signalling word boundaries change the encoding and, in adults, if it varies across modalities. In six behavioural experiments, we show that: (i) Adults and infants learn the streams’ statistical structure. (ii) Ordinal encoding emerges in the auditory modality, and pauses enhanced it. However, (iii) ordinal encoding seems to depend on the learning stage and not on pauses marking Words’ edges. Interestingly, (iv) for visual presentation of orthographic syllables, we do not find evidence of ordinal encoding in adults. Our results support the emergence, in the auditory modality, of a Word representation where its constituents are associated with an ordinal position at play already early in life, bringing new insights into speech processing and language acquisition. Additionally, we successfully use for the first time pupillometry in an infant segmentation task.

PMID:33707004 | DOI:10.1016/j.cognition.2021.104646

Methods Cell Biol. 2021;162:353-388. doi: 10.1016/bs.mcb.2020.10.001. Epub 2020 Nov 18.

ABSTRACT

Correlative microscopy experiments require the co-registration of the image data acquired by different micro-analytical techniques. Major challenges are the potentially very different fields-of-view and resolutions as well as the multi-modality of the data. To provide microscopists with an easy-to-use software for two-dimensional image co-registration we have developed Correlia, an open source software based on ImageJ^a/Fiji,^b which is fully tailored for the registration of multi-modal microscopy data. It can handle data-sets of in principle arbitrary extent and uses classical approaches, i.e., rigid registration tools or B-spline based deformation models for the correction of both, global and local misalignments, such that a fast registration output is provided. Here we describe some of the basics of Correlia focusing on its application: firstly, registration workflows are outlined on artificial data. In the second part these recipes are applied to register correlative data acquired on an algal biofilm and a soil sample.

PMID:33707019 | DOI:10.1016/bs.mcb.2020.10.001