Nevin Manimala

Eur J Cancer. 2021 Feb 27;148:328-339. doi: 10.1016/j.ejca.2021.02.024. Online ahead of print.

ABSTRACT

BACKGROUND: Coronavirus disease (COVID-19) is interfering heavily with the screening, diagnosis and treatment of cancer patients. Better knowledge of the seroprevalence and immune response after Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection in this population is important to manage them safely during the pandemic.

METHODS: 922 cancer patients, 100 non-cancer patients and 94 health care workers (HCW) attending the Multidisciplinary Oncology Unit of Antwerp University Hospital from 24th of March 2020 till 31st of May 2020, and the Oncology Unit of AZ Maria Middelares Hospital, Ghent, from 13th of April 2020 till 31st of May 2020 participated in the study. The Alinity® (A; Abbott) and Liaison® (D; DiaSorin) commercially available assays were used to measure SARS-CoV-2 IgG, while total SARS-CoV-2 Ig was measured by Elecsys® (R; Roche).

RESULTS: In the overall study population IgG/total SARS-CoV-2 antibodies were found in respectively 32/998 (3.2%), 68/1020 (6.7%), 37/1010 (3.7%) and of individuals using the A, D or R test. Forty-six out of 618 (7.4%) persons had a positive SARS-CoV-2 polymerase chain reaction (RT-PCR) test. Seroprevalence in cancer patients (A:2.2%, D:6.2%, R:3.0%), did not significantly differ from that in non-cancer patients (A:1.1%, D:5.6%, R:0.0%), but was lower than the HCW (A:13%, D:12%, R:12%; respectively Fisher’s exact test p = 0.00001, p = 0.046, p = 0.0004). A positive SARS-CoV-2 RT-PCR was found in 6.8% of the cancer patients, 2.3% of the non-cancer patients and 28.1% of the HCW (Fisher’s exact test p = 0.0004). Correlation between absolute values of the different Ig tests was poor in the cancer population. Dichotomising a positive versus negative test result, the A and R test correlated well (kappa 0.82 p McNemar test = 0.344), while A and D and R and D did not (respectively kappa 0.49 and 0.57; result significantly different p McNemar test = <0.0001 for both). The rate of seroconversion (>75%) and median absolute antibody levels (A: 7.0 versus 4.7; D 74.0 versus 26.6, R: 16.34 versus 7.32; all >P Mann Whitney U test = 0.28) in cancer patients and HCW with a positive RT-PCR at least 7 days earlier did not show any differences. However, none (N = 0/4) of the patients with hematological tumours had seroconversion and absolute antibody levels remained much lower compared to patients with solid tumours (R: 0.1 versus 37.6, p 0.003; D 4.1 versus 158, p 0.008) or HCW (all p < 0.0001).

CONCLUSION: HCW were at high risk of being infected by SARS-CoV-2 during the first wave of the pandemic. Seroprevalence in cancer patients was low in the study period. Although Ig immune response in cancer patients with solid tumours does not differ from healthy volunteers, patients with hematological tumours have a very poor humoral immune response. This has to be taken into account in future vaccination programmes in this population. SARS-CoV-2 antibody tests have divergent results and seem to have little added value in the management of cancer patients.

PMID:33773276 | DOI:10.1016/j.ejca.2021.02.024

Eur J Obstet Gynecol Reprod Biol. 2021 Mar 20;260:131-136. doi: 10.1016/j.ejogrb.2021.03.025. Online ahead of print.

ABSTRACT

OBJECTIVE: To compare the performance of uterocervical angle (UCA) and cervical length (CL) measurement at 20 weeks of pregnancy for prediction of spontaneous preterm birth (sPTB) in twin pregnancies.

STUDY DESIGN: We conducted a retrospective cohort study of 424 twin pregnancies who delivered in our center from October 2014 to December 2018 and who underwent transvaginal ultrasound between 19⁺⁰-22⁺⁰ weeks to measure CL during routine second trimester scan. Recorded ultrasound images of CL were reassessed to evaluate UCA. Medical and obstetric data were also collected for statistical analysis.

RESULTS: A total of 424 women were included. The rates of sPTB rate below 28, 32 and 34 weeks of gestation were 2.8 %, 5.4 % and 10.4 %, respectively. ROC curves showed a better area under the curve (AUC) for UCA at all gestational ages compared with CL (AUC for sPTB <28 weeks 0.902 (p < 0.001) vs 0.620 (p 0.175); AUC for sPTB <32 weeks 0.740 (p 0.001) vs 0.620 (p 0.058); AUC for sPTB <34 weeks 0.676 (p 0.001) vs 0.632 (p 0.047). UCA > 120 degrees was significantly associated with sPTB <28 weeks (p < 0.001; OR 39.17; CI 4.81-319.23; NPV, 99.65 %), <32 weeks (OR 4.23; p 0.002) and <34 weeks of gestation (OR 2.66; p 0.01).

CONCLUSION: In our study, an UCA > 120 degrees allowed to identify those women with twin pregnancies at risk of sPTB and performed better than CL measurement.

PMID:33773259 | DOI:10.1016/j.ejogrb.2021.03.025

J Hazard Mater. 2021 Mar 11;415:125631. doi: 10.1016/j.jhazmat.2021.125631. Online ahead of print.

ABSTRACT

The exposure of soil to metals and to antibiotic resistant bacteria may lead to the progressive deterioration of soil quality. The persistence of antibiotic resistant bacteria or antibiotic resistance genes in soil can be influenced by the microbial community or by soil amendments with metal salts. This work assessed the effect of soil amendment with copper and zinc, as sulfate or nitrate salts, on the fate of a carbapenem-resistant (bla_VIM⁺) hospital effluent isolate of Pseudomonas aeruginosa (strain H1FC49) and on the variations of the microbial community composition. Microcosms with soil aged or not with copper and zinc salts (20 mM), and inoculated with P. aeruginosa H1FC49 were monitored at 0, 7, 14 and/or 30 days, for community composition (16S rRNA gene amplicon) and strain H1FC49 persistence. Data on culturable P. aeruginosa, quantitative PCR of the housekeeping gene ecf, and the presumably acquired genes bla_VIM⁺ and integrase (intI1), and community composition were interpreted based on descriptive statistics and multivariate analysis. P. aeruginosa and the presumably acquired genes, were quantifiable in soil for up to one month, in both metal-amended and non-amended soil. Metal amendments were associated with a significant decrease of bacterial community diversity and richness. The persistence of P. aeruginosa and acquired genes in soils, combined with the adverse effect of metals on the bacterial community, highlight the vulnerability of soil to both types of exogenous contamination.

PMID:33773246 | DOI:10.1016/j.jhazmat.2021.125631

Plant Physiol Biochem. 2021 Mar 15;162:592-602. doi: 10.1016/j.plaphy.2021.03.024. Online ahead of print.

ABSTRACT

The expression stability of six commonly used housekeeping genes (18S rRNA-18S ribosomal RNA, EF1α-elongation factor 1α, ACT1-Actin 1, GAPDH-Glyceraldehyde-3-phosphate dehydrogenase, TUB6-Tubulin/FtsZ family and UBC-Ubiquitin-conjugating enzyme) were scrutinized in leaves and roots of Cd stressed pigeonpea plants inoculated with arbuscular mycorrhizal (AM) species- Rhizoglomus intraradices (Ri), Funneliformis mosseae (Fm), Claroideoglomus etunicatum (Ce), C. claroideum (Cc). The stability profile of each gene was assessed using ΔC_t, BestKeeper, NormFinder, RefFinder and geNorm algorithmic programs, which ranked different genes as most and least stable according to the tissues analysed. All the statistical algorithms ranked TUB6 as most stable and EF1α least stable housekeeping (HK) genes in both the plant tissues. The selected HK genes were verified using metallothionein (CcMT1) i.e. a stress responsive gene, whose expression altered under conditions of metal stress and AM inoculation. The expression pattern of CcMT1 varied highly when least stable reference gene was used for normalization as compared to most stable gene, under different treatments. Thus, there is a need of selecting suitable reference gene to achieve reliable results in gene expression studies using quantitative real time PCR (qRT-PCR). The study conducted will help future gene expression analysis in pigeonpea under specific stress.

PMID:33773234 | DOI:10.1016/j.plaphy.2021.03.024

J Environ Manage. 2021 Mar 24;288:112384. doi: 10.1016/j.jenvman.2021.112384. Online ahead of print.

ABSTRACT

Groundwater resources in South Asian cities are facing immense stress due to over-extraction leading to environmental, social and economic instabilities. The perennial mega rivers of Himalayas form the lifeline for South Asia, underpinning food and water security for a large population both directly and indirectly through exchange with groundwater systems. The present study delineates the spatio-temporal variation in patterns and processes of sub-hourly to annual-scale hydrological exchanges between the Ganges and its adjoining highly exploited aquifer in a urban-peri urban reach. Multivariate statistical analyses established river water-groundwater interaction in this region with ~40% loading of first principal component, i.e river water during monsoon on the shallow aquifer. The part of the aquifer detached from the main confined aquifer show an influence of precipitation (the second principal component) with loading of ~90%. Again the part of the aquifer suffering infiltration of local surface water bodies show effect of precipitation with a second principal loading of ~80%. Fourier transformation is used in the hydrograph to remove influence of heavy urbanization on the hydrographs. This study proves that the phenomenon of infiltrating river water during monsoon plays a primary role in controlling aquifer storage although contaminating the aquifer simultaneously. However, during pre and post-monsoon the flow path reversal helps in maintaining river baseflow. Cross-correlation between the river and piezometric series show increased delay of pressure head propagation of the infiltrating river waterfront, with increasing distance. These observations are also substantiated by stable isotope signatures. The present study provides an understanding of potential groundwater vulnerability resulting from waste water and irrigational contamination through river water intrusion which would eventually lead the government to implement proper water and environmental management policies towards availability of long-term sustainable water resources for the residents.

PMID:33773213 | DOI:10.1016/j.jenvman.2021.112384

J Environ Manage. 2021 Mar 24;288:112368. doi: 10.1016/j.jenvman.2021.112368. Online ahead of print.

ABSTRACT

A better knowledge of surface ozone variations and the relevant influential factors is of great significance for controlling frequent ozone pollution events. In this study, we first examined the primary variation patterns of surface ozone in space and time across China via a clustering analysis on the basis of daily maximum 8h average surface ozone (MDA8) between 2015 and 2018. Statistical models were then established between MDA8 and a set of influential factors to pinpoint dominant factors contributing to regional MDA8 variations. The clustering results revealed four typical variation patterns of MDA8 in China given distinct pollution levels, seasonality, and long-term trends. Statistical modeling results indicated that the seasonal variability of MDA8 was closely associated with UV radiation and meteorological factors like boundary layer height, temperature and relative humidity. In contrast, the long-term trends of MDA8 were largely linked to ozone precursors and meteorological variables including temperature, relative humidity, and total cloud cover. Moreover, the phenomenal increasing trends of MDA8 in North China were found to be statistically associated with the depletion of nitrogen dioxide (NO₂) and carbon monoxide (CO). Specifically, substantial increases in volatile organic compounds (VOCs) along with depletions in NO₂ and CO significantly boosted the photochemical ozone formation chain process in a VOC-limited regime like the North China plain. Overall, the inferred linkage in this study provides evidence and clues to help control increasing ozone pollution events in North China.

PMID:33773209 | DOI:10.1016/j.jenvman.2021.112368

Int Immunopharmacol. 2021 Mar 24;95:107575. doi: 10.1016/j.intimp.2021.107575. Online ahead of print.

ABSTRACT

BACKGROUND: Metformin (MET) may exert anti-rheumatic effects and reduce cartilage degradation through its immunomodulatory and anti-inflammatory actions.

METHODS: This was a double-blind placebo-controlled study, 120 adult patients with active rheumatoid arthritis (RA) were randomized to receive MET (1000 mg) or placebo daily with methotrexate (MTX, 7.5 mg/week) for 12 weeks. American College of Rheumatology (ACR)20, ACR50, and ACR70 response rates, Disease Activity Score in 28 joints (DAS-28), and drug safety were the efficacy endpoints. Serum levels of TNF-α, IL-1β, IL-6, IL-10, IL-17A, NF-κB, TGG-β1, MDA together with gene expression of AMPK and IGF-IR were assessed before and after the therapy.

RESULTS: A total of 80.8% of the patients in the MET group, compared with 54.7% in placebo group, met the criteria of ACR20 response after 12 weeks (P = 0.001). Statistically significant enhancements in the DAS28-3 (CRP) were observed after 4 and 8 weeks for the MET group compared with placebo and were sustained after 12 weeks. MET group showed statistically significant increase in percentage of patients achieving DAS remission after 12 weeks (P = 0.015). Significant improvements in ACR50, ACR70, Health Assessment Questionnaire Disability Index (HAQ-DI), and DAS28-3 (CRP) were also reported. MET was well-tolerated, and no serious adverse effects were reported in both groups. Furthermore, the MET group was superior in improving the measured parameters compared to the placebo.

CONCLUSIONS: MET improved the anti-rheumatic effect of MTX; suggesting it to be a beneficial adjuvant in patients with RA. Trial registration ID: NCT04068246.

PMID:33773207 | DOI:10.1016/j.intimp.2021.107575

Accid Anal Prev. 2021 Mar 24;154:106082. doi: 10.1016/j.aap.2021.106082. Online ahead of print.

ABSTRACT

Pedelecs (pedal electric cycles) have become increasingly popular in recent years. This increase may trigger many positive effects, but unfortunately it also results in an increase in accidents, especially single accidents, involving these vehicles. This investigation analyses 1738 free text descriptions of pedelec single accidents written by police officers on site. The accidents occurred in 2016 and 2017 in 14 federal German states. The aim is to evaluate whether pedelec single accidents have specific causes that have not yet been recorded in the official accident statistics. Results show that most cases can be assigned to categories addressing the user (60 %) or the road design (54 %, accidents can be assigned to several categories). With this dataset it is also possible to analyse the severity of injuries using ordered probit analysis. Of the nine categories where severe injuries are expected, most address the user. In addition, serious injuries are expected when problems are encountered on downhill slopes or involve technical faults. An additional ordered probit analysis with a second dataset, based on different sources but using the same categorisation, supports these results.

PMID:33773198 | DOI:10.1016/j.aap.2021.106082

Ecotoxicol Environ Saf. 2021 Mar 24;215:112149. doi: 10.1016/j.ecoenv.2021.112149. Online ahead of print.

ABSTRACT

The aim of present work was to evaluate the effects of titanium dioxide nanoparticles (TiO₂ NPs) on rice’s growth (Oryza sativa L.) and nutrient availability under different soil textures. Greenhouse experiment was carried out with three soil textures (sandy loam, silt loam and silty clay loam) and two concentrations of TiO₂ NPs (500, 750 mg kg^-1). Control (without TiO₂ NPs) was also maintained for the comparison. Growth parameters including chlorophyll content, root/shoot length, fresh/dry biomass and nutrients’ uptake including calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), phosphorous (P), potassium (K) and zinc (Zn) were determined. The results revealed that application of 500 mg kg^-1 TiO₂ NPs in silty clay loam soil increased the chlorophyll content (3.3-folds), root length (49%), shoot length (31%), root and shoot biomass (41% & 39%, respectively) as compared to other soil textures. The maximum plant growth was observed in silty clay loam > silt loam > sandy loam. Concentration of Cu, Fe, P and Zn in shoot was increased by 8 – , 2.3 – , 0.4 – , 0.05 -folds in silty clay loam upon 500 mg kg^-1 TiO₂ NPs application as compared to the control. Backward selection method to model the parameters (nutrients in soil) for the response variables (root/shoot length and biomass) showed that Ca, Fe, P are the main nutrients responsible for the increase in plant length and biomass. Overall, the growth of rice was better in silty clay loam at 500 mg kg^-1 of TiO₂ NPs.

PMID:33773153 | DOI:10.1016/j.ecoenv.2021.112149

Ecotoxicol Environ Saf. 2021 Mar 24;215:112160. doi: 10.1016/j.ecoenv.2021.112160. Online ahead of print.

ABSTRACT

BACKGROUND: The air pollution has become an important environmental health problem due to its adverse health effect. The objective of this study was to investigate the effects of ambient temperature and pollutants on mortality of respiratory diseases (RD) in Hefei, China, a typical inland city.

METHODS: Nonlinear exposure-response dependencies and delayed effects of urban daily mean temperature (DMT) and pollutants were evaluated by distributed lag non-linear models (DLNM). To further explore this effect, different genders and ages were also examined by stratified analysis.

RESULTS: A total of 12876 deaths from RD were collected from January 1, 2014 to December 31, 2018 in Hefei, China. There was a U-shaped correlation between DMT and RD mortality, and the RD mortality rised by 11.6% (95% CI: 2.2-22.0%) when the DMT was 35.8 °C (reference temperature is 20 °C). The results show that risk of death with short-term exposure to elevated concentrations of PM₁₀ and SO₂ was not significant. The maximum hysteresis and cumulative relative risk (RR) of RD mortality were 1.012 (95% CI: 1.003 ~ 1.021, lag 0 day) and 1.072 (95% CI: 1.014 ~1.133, lag 10 days) for each 10 μg/m³ augment in NO₂; 1.005 (95% CI: 1.001-1.009, lag 0 day) and 1.027 (95% CI: 1.004-1.051, lag 10 days) for each 10 μg/m³ augment in O_3; a negative association between CO exposure and the cumulative risk of death was observed (RR = 0.964, 95% CI: 0.935-0.993, lag 07 days). Subgroup analysis showed the effect of high temperatures, NO_2, O₃ and CO exposure was still statistically significant for the elderly and male.

CONCLUSION: The present study found that short-term exposure to high temperature, NO_2, O₃ and CO were significantly associated with the risk of RD mortality and male as well as elderly are more susceptible to these factors.

PMID:33773152 | DOI:10.1016/j.ecoenv.2021.112160