Category: Statistics

J Contemp Dent Pract. 2022 May 1;23(5):497-502.

ABSTRACT

AIM: The purpose of the study was to evaluate the expression of stathmin in different histological grades and tumor, node, metastasis (TNM) staging of Oral carcinoma and various grades of oral dysplasia. The study also aims at observing the stathmin expression with respect to lymph node metastasis.

MATERIALS AND METHODS: A total of 90 histopathologically confirmed tissue sections were acquired, of which 30 sections of oral dysplasia, 30 oral squamous cell carcinoma (OSCC) and 30 normal tissue sections were stained immunohistochemically with stathmin. The tissue sections, were categorized into different grades of oral dysplasia and OSCC based on histopathological examination. For estimation of stathmin expression, manual examination of 300 cells was done in a minimum of five different areas of tissue section and a mean proportion of positive-stained cells were determined. The statistical analysis of the results was done using ANOVA test.

RESULTS: A statistically significant increase in mean staining scores of stathmin in OSCC group compared to dysplasia and control groups. A statistically significant difference was observed in different grades of dysplasia and OSCC groups. Stage III and stage IV OSCC tissue sections showed high intensity staining scores of stathmin expression.

CONCLUSION: An increased expression of stathmin was detected in various grades of OSCC and also with respect to staging of oral cancer. Half the cases of OSCC with lymph node metastasis showed high intensity scores of stathmin. Based on the above facts, stathmin expression was indicated as a potential tool for predicting the outcome of oral cancer patients with lymph node metastasis and its expression was increased in the group with poor prognosis.

CLINICAL SIGNIFICANCE: Any damage/mutation to stathmin can result in defects in cell division resulting in aneuploidy and in turn cancers. In this study, the results showed that there is a differential expression of stathmin in the early and the advanced grades and different TNM stages of OSCC. A high expression of stathmin was observed in all the cases with lymph node metastasis. These observations prove that stathmin has an important role in the progression, tumorigenicity, and prognosis of the oral cancer.

PMID:35986456

J Contemp Dent Pract. 2022 May 1;23(5):492-496.

ABSTRACT

AIM: The aim of the study was to compare the bond strength of three cantilever resin-bonded bridge (RBB) designs cemented with resin cements.

MATERIALS AND METHODS: Twenty-four extracted human mandibular canines with eight teeth per group were used in this study to evaluate the bond strength of cantilever resin-retained bridge designs of the mesh, perforated, and combination of mesh and perforated luted to the prepared lingual surface of canine teeth using resin cement. Debonding was done using Instron universal testing machine by applying load on the mid-buccal region of the pontic and the obtained values were evaluated. The data was analyzed statistically.

RESULTS: The mean shear bond strengths of mesh, perforated, and combination of mesh and perforated are 0.88 ± 0.31 MPa, 0.81 ± 0.31 MPa, and 0.93 ± 0.32 MPa. However, there is no significant differences in the statistical analysis that were performed using a one-factor analysis of variant (ANOVA) test (p = 0.744).

CONCLUSION: Within the limitation of this study for cantilever resin-retained bridge designs, the combination of mesh and perforated design showed greater mean shear bond values when compared with the mesh design and perforated designs. This shows equal in vitro performance to the gold standard designs (group A is mesh design and group B is perforated design).

CLINICAL SIGNIFICANCE: The new design which is the combination of mesh and perforated shows equal in vitro performance to the gold standard designs. Thus, their use in clinical situation can bring better result in concern to cantilever resin-retained prosthesis.

PMID:35986455

J Basic Clin Physiol Pharmacol. 2022 Aug 22. doi: 10.1515/jbcpp-2022-0179. Online ahead of print.

ABSTRACT

OBJECTIVES: The aim of this study was to investigate the role of preoperative Monocyte-to-Lymphocyte ratio (MLR) as a potential predictor of bladder cancer (BC).

METHODS: Clinical data of patients who underwent TURBT at our institution between 2017 and 2021 were collected and retrospectively analysed. MLR was obtained from preoperative blood analyses performed within 1 month from hospital admission. The association of MLR with different clinic-pathological features obtained from histological reports was further analysed. Statistical analysis was performed using the Kruskal Wallis test for non-parametric variables, assuming p<0.05 as statistically significant.

RESULTS: 510 patients were included in the study (81% males, 19% females), with a mean age of 71.66 ± 11.64 years. Mean MLR was higher in patients with any-type bladder cancer, reporting an MLR of 0.41 ± 0.11 compared to 0.38 ± 0.43 in patients without bladder cancer (p=0.043). In the subsequent comparison among low-grade and high-grade bladder cancer, MLR did not report statistically significant differences, with 0.29 ± 0.12 for low-grade BC and 0.51 ± 0.81 for high-grade BC (p=0.085).

CONCLUSIONS: Our findings reported elevated preoperative MLR should be considered a potential biomarker predicting malignancy for bladder tumours. Furthermore, research are necessary to assess its role in discerning low-grade from high-grade patients.

PMID:35985034 | DOI:10.1515/jbcpp-2022-0179

Breastfeed Med. 2022 Aug 18. doi: 10.1089/bfm.2022.0056. Online ahead of print.

ABSTRACT

Objective: Breast milk is the best nutrient for newborns due to its rich dietary content, immunological factors, ease of accessibility, and affordability. Given the fact that adolescent birth rate in Turkey is high, this study examines the effects of adolescent motherhood with regard to macronutrient contents in breast milk. Study Design: We collected colostrum samples from mothers staying at our hospital. Gestational ages were ≥37 weeks. Demographic characteristics of the patients were registered. The MIRIS (Mid-Infrared Human Milk Analyzer) device was operated to analyze breast milk, adopting a spectroscopy method for measurements. Protein, fat, carbohydrate, and energy levels of the hindmilk samples were measured; thus, study groups were compared. Results: The study included 224 mothers, of whom 49 were adolescents and 125 were adults. Maternal weight and maternal body mass index, weight gain during pregnancy, mode of delivery and gestational age, as well as birth weight and gender of the infants were similar. As breast milk contents were compared, we found out that fat content levels in the colostrum of adolescent mothers were significantly higher, with respect to the other group. Protein and carbohydrate levels were lower in adolescents, even if they were not statistically significant. There was no difference between the groups in terms of energy levels. Conclusion: Adolescent motherhood may have negative influences on infant welfare. However, in terms of breast milk content quality, adult mothers are not superior to adolescents.

PMID:35985000 | DOI:10.1089/bfm.2022.0056

Health Rep. 2022 Aug 18;33(8):19-30. doi: 10.25318/82-003-x202200800002-eng.

ABSTRACT

BACKGROUND: Mental health among Canadians has worsened since the start of the COVID-19 pandemic. The purpose of this study was to identify profiles of mental health difficulties and to quantify the relationships between mental health profiles, negative impacts related to the pandemic and suicidal ideation.

DATA AND METHODS: Participants were 22,721 adults (18 years and older) from the 2020 and 2021 Survey on COVID-19 and Mental Health. Latent profile analysis was used to identify patterns of anxiety, depression and psychological distress. The relationships between mental health profiles, negative impacts and suicidal ideation were examined using logistic regression models.

RESULTS: Three mental health profiles were identified. Individuals were classified as having no mental health difficulties (Profile 1, 65.70%), low-to-moderate mental health difficulties (Profile 2, 25.52%) and severe mental health difficulties (Profile 3, 8.78%). Individuals in Profiles 2 and 3 were at greater odds than individuals in Profile 1 of experiencing emotional distress; the death of a family member, friend or colleague; difficulty in meeting financial obligations or essential needs; the loss of a job or income; feelings of loneliness or isolation; physical health problems; challenges in personal relationships with household members; and other impacts. Individuals in Profile 2 (4.27%, odds ratio (OR) = 24.30) and Profile 3 (19.09%, odds ratio (OR) = 115.75) were considerably more likely to have contemplated suicide since the onset of the pandemic compared with those in Profile 1 (0.16%).

INTERPRETATION: Individuals who experienced high levels of anxiety, depression and psychological distress were most vulnerable to negative impacts related to the pandemic and suicidal ideation.

PMID:35984951 | DOI:10.25318/82-003-x202200800002-eng

Health Rep. 2022 Aug 18;33(8):31-38. doi: 10.25318/82-003-x202200800003-eng.

ABSTRACT

BACKGROUND: Globally, the suicide rate is two times higher for males than for females. Previous studies in Newfoundland and Labrador did not examine age-specific rates by sex. The objectives of this study were to determine suicide rates by sex and age group and to compare the demographic and clinical characteristics of males and females who died by suicide.

DATA AND METHODS: This observational study analyzed a routinely collected dataset based on all medical examiner-determined suicide deaths among people aged 10 years and older in Newfoundland and Labrador, Canada, between 1997 and 2016. Age-standardized and age-specific suicide rates and rate ratios were calculated based on the number of deaths during the period, and descriptive statistics were used to compare demographic and clinical characteristics between males and females.

RESULTS: The age-standardized suicide rate was 4.6 times higher among males than females and was higher for males in most age groups. Rates were highest in the young adult age groups for males (20 to 24 years) and females (35 to 39 years). Males who died by suicide were more likely to be from a rural community and to have died by firearm; females were more likely to die by self-poisoning and to have had a mental illness or substance use history.

INTERPRETATION: The results are broadly consistent with previous research, though this is the first study to report age-specific suicide rates among females across the life course in Newfoundland and Labrador. The results underscore the need to design public health and clinical interventions that account for sex differences in suicide risks.

PMID:35984952 | DOI:10.25318/82-003-x202200800003-eng

Health Rep. 2022 Aug 18;33(8):3-18. doi: 10.25318/82-003-x202200800001-eng.

ABSTRACT

BACKGROUND: Recently, the Canadian 24-Hour Movement Guidelines for Adults were released, and included a revised physical activity (PA) recommendation. The recommendation of 150 minutes per week of moderate-to-vigorous intensity PA (MVPA) was revised, from requiring that MVPA be accrued in bouts of 10 minutes or more (bouted) to having no bout requirement (non-bouted). The objective of this study was to assess whether there were differences in sociodemographic, health and fitness characteristics of Canadians who met the bouted and non-bouted PA recommendations.

DATA AND METHODS: Using adult (aged 18 to 79 years) accelerometer data from three combined cycles of the nationally representative Canadian Health Measures Survey (N = 7,102), this study compared adherence to the bouted and non-bouted recommendations. Differences in sociodemographic, health and fitness measures were assessed using independent t-tests and chi-squares. Multivariate linear and logistic regressions controlling for age, sex, household education and smoking examined associations with health and fitness measures.

RESULTS: More adults met the PA recommendation using the non-bouted versus bouted (45.3% vs. 18.5%) requirement. Characteristics of those who met the bouted and only the non-bouted recommendations were similar. Exceptions among those who met only the non-bouted recommendation compared with meeting the bouted recommendation included fewer adults aged 65 years and older; lower MVPA, recreation PA and transport PA; and higher sedentary time, light PA and grip strength.

INTERPRETATION: Although the removal of the 10-minute bout requirement increased the proportion of Canadian adults who met the PA recommendation, there were no substantial differences in the sociodemographic and health characteristics of the populations captured by the bouted and non-bouted definitions. Results help to inform the transition in reporting for PA surveillance.

PMID:35984950 | DOI:10.25318/82-003-x202200800001-eng

J Aerosol Med Pulm Drug Deliv. 2022 Aug 18. doi: 10.1089/jamp.2022.0006. Online ahead of print.

ABSTRACT

Background: Suboptimal use of pressurized metered dose inhaler (pMDI) remains a major barrier to inhaled therapy success. Verbal inhaler technique training (VT) fails to maintain patients’ good pMDI use, thus training tools might help. Trainhaler^® (THR device) and Flo-Tone^® CR (FTCR device), two novel pMDI technique training tools, were evaluated and compared in terms of relative lung and systemic bioavailability and oropharyngeal deposition of salbutamol inhaled from Ventolin^® Evohaler^® (GlaxoSmithKline) either alone following THR or connected to FTCR. Methods: Sixteen healthy adults inhaled 2 × 100 μg salbutamol puffs (1 minute apart) from Ventolin using the THR device or FTCR device in a two-period, randomized crossover study. A 7-day washout separated THR and FTCR approaches. Immediately after each puff inhalation, each subject gargled with 20 mL water for oropharyngeal deposition determination. Urine samples were collected 0.5 hour (pre-inhalation) and 0.5, 1.0, and 2.0 hours post-inhalation. Urine was then pooled till 24-hour post-inhalation. The relative lung bioavailability (0- to 0.5-hour urinary salbutamol excretion-USAL0.5) and relative systemic bioavailability (0- to 24-hour urinary excretion of salbutamol and its metabolite-USALMET24) were determined. Results: The mean (standard deviation [SD]) USAL0.5 of the THR and FTCR groups was 5.70 (6.43) and 11.39 (9.67) μg, respectively. The mean (SD) oropharyngeal deposition was 11.11 (4.37) and 6.09 (1.89) μg, respectively. The THR and FTCR devices were statistically significantly different in USAL0.5 and oropharyngeal deposition (p < 0.001), whereas there was no statistically significant difference in USALMET24. Conclusion: The THR device and the FTCR device showed positive impact on inhaled pMDI delivery. Indeed, the FTCR device doubled the relative lung bioavailability and minimized the unwanted oropharyngeal deposition of inhaled salbutamol. In practice, these pMDI trainers would complement and maintain VT. Study Registration: The study was registered on the ISRCTN registry (Reference: ISRCTN88332465-06/12/2017 [Prospectively Registered]).

PMID:35984934 | DOI:10.1089/jamp.2022.0006

Expert Rev Respir Med. 2022 Aug 19. doi: 10.1080/17476348.2022.2115361. Online ahead of print.

ABSTRACT

BACKGROUND: The results of associations between single nucleotide polymorphisms (SNPs) of genes in DNA repairing pathway and lung cancer (LC) risk are inconsistent.

METHODS: We applied allele, dominant and recessive models to explore the risk of researched variants to LC in total LC and subgroups by ethnicity or LC subtypes with a cutoff point of p <0.05.

RESULTS: 76935 cases and 88649 controls from 192 articles were included. Among the analyzed 40 variants from 20 genes, we found 9 statistically significant variants in overall populations by allele model, including five SNPs (rs1760944, rs9344, rs13181, rs1001581 and rs915927) increasing LC risk (odd ratios [ORs]=1.10-1.71) and four SNPs (rs1042522, rs3213245, rs11615 and rs238406) decreasing the risk (ORs=0.75-0.94). We identified rs1042522 and rs13181 as significant variants for LC in three models. Additionally, we identified differential significant SNPs in ethnic and subtype’s analysis with comparison to total population.

CONCLUSIONS: There are five SNPs in DNA repairing pathway associated with increased LC risk and four others decreased LC risk. Besides, the risky SNPs in different ethnicities and various LC subtypes were partly different, and the contribution of different genotypes to risk alleles were various as well.

PMID:35984915 | DOI:10.1080/17476348.2022.2115361

ACS Appl Mater Interfaces. 2022 Aug 19. doi: 10.1021/acsami.2c09910. Online ahead of print.

ABSTRACT

Well-defined block copolymers have been widely used as emulsifiers, stabilizers, and dispersants in the chemical industry for at least 50 years. In contrast, nature employs amphiphilic proteins as polymeric surfactants whereby the spatial distribution of hydrophilic and hydrophobic amino acids within the polypeptide chains is optimized for surface activity. Herein, we report that polydisperse statistical copolymers prepared by conventional free-radical copolymerization can provide superior foaming performance compared to the analogous diblock copolymers. A series of predominantly (meth)acrylic comonomers are screened to identify optimal surface activity for foam stabilization of aqueous ethanol solutions. In particular, all-acrylic statistical copolymers comprising trimethylhexyl acrylate and poly(ethylene glycol) acrylate, P(TMHA-stat-PEGA), confer strong foamability and also lower the surface tension of a range of ethanol-water mixtures to a greater extent than the analogous block copolymers. For ethanol-rich hand sanitizer formulations, foam stabilization is normally achieved using environmentally persistent silicone-based copolymers or fluorinated surfactants. Herein, the best-performing fully hydrocarbon-based copolymer surfactants effectively stabilize ethanol-rich foams by a mechanism that resembles that of naturally-occurring proteins. This ability to reduce the surface tension of low-surface-energy liquids suggests a wide range of potential commercial applications.

PMID:35984897 | DOI:10.1021/acsami.2c09910